Silybum marianum L. Gaertner'in Antioksidan Kapasitesi, Toplam Fenolik ve Flavonoid İçerikleri

Öz

Terapötik özelliklerinden dolayı Silybum marianum'un toprak üztünde kalan kısımları geleneksel olarak halk hekimliğinde kullanılmaktır. Bu çalışmanın amacı, Silybum marianum toprak üstü kısımlarının metanol ekstraktlarının antioksidan kapasitesini ve toplam fenol ve flavonoid içeriklerini belirlemektir. Silybum marianum'un antioksidan kapasitesini belirlemek için DPPH (2, 2-difenil-1-pikrilhidrazil) serbest radikal süpürme ve indirgeme kapasitesi deneyleri kullanıldı. Test edilen tüm ekstraktlar, önemli ölçüde toplam fenolik ve toplam flavonoid içeriğinin yanı sıra güçlü antioksidan kapasite göstermiştir. Metanolik ekstraktın, bir miktar fenol (kurutulmuş materyalin gramı başına 56.95±0.78 µmg gallik asit eşdeğeri) ve flavonoidler (ekstrenin gramı başına 86.09±0.35 µmg kersetin eşdeğeri) içerdiği bulundu. Ayrıca aynı ekstraktın önemli ölçüde daha yüksek DPPH indirgeme gücüne ve süpürme kapasitesine (İnhibisyon % =  91.77±0.12) sahip olduğu gözlenmiştir. Bu bulgular, Silybum marianum toprak üstünde kalan kısımlarının gıda, ilaç ve kozmetik endüstrilerinde doğal bir antioksidan kaynağı olarak kullanılabileceğini göstermektedir.

Anahtar Kelimeler

• Antioksidan kapasitesi
• Silybum marianum
• total flavonoid
• total fenol

Total phenol and flavonoid contents, and antioxidant capacity of Silybum marianum L. Gaertner grown in Turkey

Abstract

Because of their therapeutic properties, the aerial parts of Silybum marianum have traditionally been used in folk medicine. The purpose of this study was to determine the antioxidant capacity and total phenol and flavonoid contents of methanol extracts of Silybum marianum aerial parts. DPPH (2, 2-diphenyl-1-picrylhydrazil) free radical scavenging and reducing power assays were used to assess the antioxidant capacity of Silybum marianum. The total phenol and flavonoid contents of each extract were determined using the Folin-Ciocalteu and aluminum trichloride methods, respectively. DPPH (2, 2-diphenyl-1-picrylhydrazil) free radical scavenging assays were used to assess the antioxidant capacity of Silybum marianum extracts. All of the extracts tested showed impressive total phenolic and total flavonoid content, as well as potent antioxidant capacity. The methanolic extract was found to be containing a number of total phenols (56.95±0.78  mg of gallic acid equivalent per gram of dried material) and flavonoids (86.09±0.35 mg of quercetin equivalent per gram of extract). The same extract also had a significantly higher DPPH-reducing power and scavenging capacity (Inhibition % =  91.77±0.12). These findings imply that Silybum marianum aerial parts could be used as a natural source of antioxidants in the food, pharmaceutical, and cosmetic industries.

Keywords

• Antioxidant capacity
• Silybum marianum
• total flavanoid
• total phenol

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