Antimicrobial and Antioxidative Properties of Kombucha Teas Produced with Black and Green Tea
Year 2018,
, 96 - 101, 31.12.2018
Gökhan Akarca
,
Oktay Tomar
Abstract
In this study, it is aimed to determine the anticrobial and antioxidative properties of the Kambucha teas produced by two different teas at the end of the fermentation period. At the end of the fermentation period of 18 days, it was determined that the free radical removal activity (% 62.4 ± 0.8) and total phenolic content (7.8 ± 1.3 GAE, mM) of teas produced with black tea were higher than those produced with green tea. The antimicrobial activity test on 8 different foodborne pathogenic bacteria was found to have the highest effect on the Staphylococcus aureus (24 mm zone diameter) in the Kambucha teas produced by black tea, followed by the Escherichia coli (20 mm zone diameter). MIC values of the same bacteria were determined as 24 and 48 μg / mL, respectively.
References
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- Battikh H., Bakhrouf A. and Ammar E. 2012. Antimicrobial effect of Kombucha analogue. LWT - Food Science and Technology. 47, 71-77.
- Bronner W.E. and Beecher G.R. 1981. Method for determining the content of catechins in tea infusions by high performance liquid chromatography. Journal of Chromatography. 6805, 137–142.
- By Aamer A.A., Abdul-Hafeez M.M. and Sayed S.M. 2015. Minimum inhibitory and bactericidal concentrations (MIC & MBC) of Honey and bee propolis against multidrug resistant (mdr) Staphylococcus spp. isolated from bovine clinical mastitis. Global Journal of Science Frontier Research: D Agriculture and Veterinary. 15, 2 Version 1.0 .
- Chikezie I.O., 2017. Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a novel dilution tube method. African Journal of Microbiology Research. 11(23), 977-980.
- Chu S.C. and Chen C. 2006. Effects of origins and fermentation time on the antioxidant activities of Kombucha. Food Chemistry. 98, 502–507.
- Cruz-Gálvez A.M., Castro-Rosas J., Rodríguez-Marín M.L., Cadena-Ramírez A., Tellez-Jurado A., Tovar-Jiménez X., Chavez-Urbiola E.A., Abreu-Corona A. and Gómez-Aldapa C.A. 2018. Antimicrobial activity and physicochemical characterization of a potato starch-based film containing acetonic and methanolic extracts of Hibiscus sabdariffa for use in sausage. LWT Food Science and Tecnology. 93, 300-305. https://doi.org/10.1016/j.lwt.2018.02.064.
- Dipti P., Yogesh B., Kain A.K., Pauline T., Anju B., Sairam, M., Singh B., Mongia S.S., Kumar G.I. and Selvamurthy W. 2003. Lead induced oxidative stress: beneficial effects of Kombucha tea. Biomedical and Environmental Sciences. 16, 276–282.
- Dutta D. and Gachhui R. 2006. Novel nitrogen-fixing Acetobacter nitrogenifigens spp nov., isolated from Kombucha tea. Int. J. Syst. Evol. Microbiol. 56, 1899-1903.
- El-Mahmood M.A., 2009. Antibacterial efficacy of stem bark extracts of Mangifera indica against some bacteria associated with respiratory tract infections. Sci Res Essays. 4(10), 1031-1037.
- El-Salam S.S.A., 2012. 16S rRNA gene sequence detection of acetic acid bacteria isolated from tea Kombucha. New York Sci. J. 5, 55-61.
- Frank G., 1995. Kombucha, healty beverage and natural remedy from the far east, Great Britain Published, Wilhelm Ennsthaler, Steyr.Jayabalan R., Malba_sa R.V., Lon_car E.S., Vitas J.S. and Sathishkumar M. 2014. A review on Kombucha tea - microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus. Compr. Rev. Food Sci. Food Saf. 13, 538 - 550.
- Jayabalan R., Malbaša R. and Sathishkumar M. 2016. Kombucha. reference module in food sciences. Elsevier 1–8.
- Kim E.S., Liang Y.R., Jin J., Sun Q.F., Lu J.L., Du Y.Y. and Lin D.C. 2007. Impact of heating on chemical compositions of green tea liquor. Food Chemistry. 103, 1263-1267.
- Marsh A.J., O'Sullivan O., Hill C., Ross R.P. and Cotter P.D. 2014. Sequence-based analysis of the bacterial and fungal compositions of multiple Kombucha (tea fungus) samples. Food Microbiol. 38, 171-178.
- Jayabalan R.S., Marimuthu S. and Swaminathan K. 2007. Changes in content of organic acids and tea polyphenols during kombucha tea fermentation, Food Chemistry 102, 392–398.
- Reva ON, Zaets I.E., Ovcharenko L.P., Kukharenko O.E., Shpylova S.P., Podolich O.V., de Vera J.-P. and Kozyrovska N.O. 2015. Metabarcoding of the Kombucha microbial community grown in different microenvironments. Amb. Express 5, 35.
- Roussin M.R., 1996. Analyses of Kombucha ferments: report on growers. Salt Lake City, Utah: Information Resources, LC.
- Sreeramulu G., Zhu Y. and Knol K. 2001. Characterization of antimicrobial activity in Kombucha fermentation. Acta Biotechnologica. 21,49- 56.
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- Stoner D.G. and Mukhtar H., 1995. Polyphenols as cancer chemopreventive agents. Journal of Cellular Biochemistry. 22, 169–180.
- Su Y.L., Leung L.K., Huang Y. and Chen Z., 2003. Stability of tea theaflavins and catechins. Food Chemistry. 83, 189–195.
- Trovatti E., Serafim L.S., Freire C.S.R., Silvestre A.J.D. and Neto C.P. 2011. Gluconacetobacter sacchari: an efficient bacterial cellulose cell-factory. Carbohydr. Polym. 86, 1417 - 1420.
- Vitasa J.S., Cvetanovića A.D., Maškovićb P.Z., Švarc-Gajića J.V. and Malbašaa R.V. 2018. Chemical composition and biological activity of novel types of Kombucha beverages with yarrow. Journal of Functional Foods. 44, 95–102.
- Yang S.C., Maliakal P. and Meng X. 2002. Inhibition of carcinogenesis by tea. Annual Review of Pharmacology and Toxicology. 42, 25–54.
- Yang S.C., Prabhu S. and Landau J. 2001. Prevention of carcinogenesis by tea polyphenols. Drug Metabolism Reviews. 33(3), 237–253.
Siyah ve Yeşil Çay İle Üretilen Kombucha Çaylarının Antimikrobiyal ve Antioksidatif Özellikleri
Year 2018,
, 96 - 101, 31.12.2018
Gökhan Akarca
,
Oktay Tomar
Abstract
Bu çalışmada iki farklı çay ile üretilen Kambucha çaylarının fermantasyon süresi sonundaki antikrobiyal ve antioksidatif özelliklerinin belirlenmesi amaçlanmıştır.18 günlük fermantasyon süresi sonunda siyah çay ile üretilen çayların Serbest radikal giderme aktivitesinin (% 62.4±0.8) ve Toplam fenolik madde miktarlarının (7.8±1.3 GAE, mM) yeşil çay ile üretilenlere kıyasla daha yüksek olduğu belirlenmiştir. 8 farklı gıda kaynaklı patojen bakteri üzerinde yapılan antimikrobiyal aktivite testi sonucunda en fazla etkinin yine siyah çay ile üretilen Kambucha çaylarında Staphylococcus aureus (24 mm zon çapı) üzerinde olduğu, bunu Escherichia coli’nin (20 mm zon çapı) izlediği tespit edildi. Aynı bakteriler üzerinde siyah çay kullanılarak üretilen çeşidin MIC değerleri ise sırasıyla 24 ve 48 μg/mL olarak belirlendi.
References
- Anonim, 2018. Eucast, European Commitee on Antimicrobial Susceptibilty Testing, http://www.eucast.org/fileadmin/src/media/PDFs/EUCAST_files/Breakpoint_tables/v_8.0_Breakpoint_Tables.pdf
- Battikh H., Bakhrouf A. and Ammar E. 2012. Antimicrobial effect of Kombucha analogue. LWT - Food Science and Technology. 47, 71-77.
- Bronner W.E. and Beecher G.R. 1981. Method for determining the content of catechins in tea infusions by high performance liquid chromatography. Journal of Chromatography. 6805, 137–142.
- By Aamer A.A., Abdul-Hafeez M.M. and Sayed S.M. 2015. Minimum inhibitory and bactericidal concentrations (MIC & MBC) of Honey and bee propolis against multidrug resistant (mdr) Staphylococcus spp. isolated from bovine clinical mastitis. Global Journal of Science Frontier Research: D Agriculture and Veterinary. 15, 2 Version 1.0 .
- Chikezie I.O., 2017. Determination of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) using a novel dilution tube method. African Journal of Microbiology Research. 11(23), 977-980.
- Chu S.C. and Chen C. 2006. Effects of origins and fermentation time on the antioxidant activities of Kombucha. Food Chemistry. 98, 502–507.
- Cruz-Gálvez A.M., Castro-Rosas J., Rodríguez-Marín M.L., Cadena-Ramírez A., Tellez-Jurado A., Tovar-Jiménez X., Chavez-Urbiola E.A., Abreu-Corona A. and Gómez-Aldapa C.A. 2018. Antimicrobial activity and physicochemical characterization of a potato starch-based film containing acetonic and methanolic extracts of Hibiscus sabdariffa for use in sausage. LWT Food Science and Tecnology. 93, 300-305. https://doi.org/10.1016/j.lwt.2018.02.064.
- Dipti P., Yogesh B., Kain A.K., Pauline T., Anju B., Sairam, M., Singh B., Mongia S.S., Kumar G.I. and Selvamurthy W. 2003. Lead induced oxidative stress: beneficial effects of Kombucha tea. Biomedical and Environmental Sciences. 16, 276–282.
- Dutta D. and Gachhui R. 2006. Novel nitrogen-fixing Acetobacter nitrogenifigens spp nov., isolated from Kombucha tea. Int. J. Syst. Evol. Microbiol. 56, 1899-1903.
- El-Mahmood M.A., 2009. Antibacterial efficacy of stem bark extracts of Mangifera indica against some bacteria associated with respiratory tract infections. Sci Res Essays. 4(10), 1031-1037.
- El-Salam S.S.A., 2012. 16S rRNA gene sequence detection of acetic acid bacteria isolated from tea Kombucha. New York Sci. J. 5, 55-61.
- Frank G., 1995. Kombucha, healty beverage and natural remedy from the far east, Great Britain Published, Wilhelm Ennsthaler, Steyr.Jayabalan R., Malba_sa R.V., Lon_car E.S., Vitas J.S. and Sathishkumar M. 2014. A review on Kombucha tea - microbiology, composition, fermentation, beneficial effects, toxicity, and tea fungus. Compr. Rev. Food Sci. Food Saf. 13, 538 - 550.
- Jayabalan R., Malbaša R. and Sathishkumar M. 2016. Kombucha. reference module in food sciences. Elsevier 1–8.
- Kim E.S., Liang Y.R., Jin J., Sun Q.F., Lu J.L., Du Y.Y. and Lin D.C. 2007. Impact of heating on chemical compositions of green tea liquor. Food Chemistry. 103, 1263-1267.
- Marsh A.J., O'Sullivan O., Hill C., Ross R.P. and Cotter P.D. 2014. Sequence-based analysis of the bacterial and fungal compositions of multiple Kombucha (tea fungus) samples. Food Microbiol. 38, 171-178.
- Jayabalan R.S., Marimuthu S. and Swaminathan K. 2007. Changes in content of organic acids and tea polyphenols during kombucha tea fermentation, Food Chemistry 102, 392–398.
- Reva ON, Zaets I.E., Ovcharenko L.P., Kukharenko O.E., Shpylova S.P., Podolich O.V., de Vera J.-P. and Kozyrovska N.O. 2015. Metabarcoding of the Kombucha microbial community grown in different microenvironments. Amb. Express 5, 35.
- Roussin M.R., 1996. Analyses of Kombucha ferments: report on growers. Salt Lake City, Utah: Information Resources, LC.
- Sreeramulu G., Zhu Y. and Knol K. 2001. Characterization of antimicrobial activity in Kombucha fermentation. Acta Biotechnologica. 21,49- 56.
- Sreeramulu G., Zhu Y. and Knol W., 2000. Kombucha fermentation and its antimicrobial activity. J. Agric. Food Chem. 48, 2589 - 2594.
- Stoner D.G. and Mukhtar H., 1995. Polyphenols as cancer chemopreventive agents. Journal of Cellular Biochemistry. 22, 169–180.
- Su Y.L., Leung L.K., Huang Y. and Chen Z., 2003. Stability of tea theaflavins and catechins. Food Chemistry. 83, 189–195.
- Trovatti E., Serafim L.S., Freire C.S.R., Silvestre A.J.D. and Neto C.P. 2011. Gluconacetobacter sacchari: an efficient bacterial cellulose cell-factory. Carbohydr. Polym. 86, 1417 - 1420.
- Vitasa J.S., Cvetanovića A.D., Maškovićb P.Z., Švarc-Gajića J.V. and Malbašaa R.V. 2018. Chemical composition and biological activity of novel types of Kombucha beverages with yarrow. Journal of Functional Foods. 44, 95–102.
- Yang S.C., Maliakal P. and Meng X. 2002. Inhibition of carcinogenesis by tea. Annual Review of Pharmacology and Toxicology. 42, 25–54.
- Yang S.C., Prabhu S. and Landau J. 2001. Prevention of carcinogenesis by tea polyphenols. Drug Metabolism Reviews. 33(3), 237–253.