Isolation and identification of the pyrethroid insecticide deltamethrin degrading bacteria from insects

Abstract

Many studies have showed that the pesticide residues in the environment increase day by day because of their continuous use. Pesticides can degrade chemically, physically and biologically. Biodegradation is an eco-friendly, inexpensive and highly effective approach compared to other methods. Bacteria are the most commonly used biological agents in biodegradation studies. Widespread use of pyrethroid pesticides such as deltamethrin causes pollution of environment. A total of 14 bacterial isolates were isolated from insects (Poecilimon tauricola, Locusta migratoria, Gryllus bimaculatus and Forficula auricularia) living in pesticide contaminated environments. These bacterial isolates were identified and characterized as Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Bacillus atrophaeus, Acinetobacter lwoffii, Rhodococcus coprophilus, Brevundimonas vesicularis, Pseudomonas syringae, Yersinia frederiksenii, Bacillus licheniformis, Enterobacter intermedius and Serratia marcescens based on biochemical and morphological properties and fatty acid profiles. As a result, these bacterial isolates can be used for the remove of deltamethrin at various environments.

Keywords

• Bacteria
• Biodegradation
• Deltamethrin
• Isolation

Böceklerden Piretroid İnsektisit Deltametrin Yıkıcı Bakterilerin İzolasyonu ve Tanımlanması

Öz

Birçok çalışma, sürekli kullanımları nedeniyle ortamdaki pestisit kalıntılarının her geçen gün arttığını göstermiştir. Pestisitler kimyasal, fiziksel ve biyolojik olarak parçanabilirler. Biyodegradasyon, diğer yöntemlere kıyasla çevre dostu, ucuz ve oldukça etkili bir yaklaşımdır. Biyodegradasyon çalışmalarında bakteriler en sık kullanılan biyolojik ajanlardır. Deltametrin gibi piretroid pestisitlerin yaygın kullanımı çevrenin kirlenmesine neden olmaktadır. Pestisit kontamine ortamlarda yaşayan böceklerden (Poecilimon tauricola, Locusta migratoria, Gryllus bimaculatus ve Forficula auricularia) toplam 14 bakteri izolatı izole edilmiştir. Bu bakteri izolatları, biyokimyasal ve morfolojik özellikleri ve yağ asidi profillerine dayanarak Pseudomonas aeruginosa, Stenotrophomonas maltophilia, Bacillus atrophaeus, Acinetobacter lwoffii, Rhodococcus coprophilus, Brevundimonas vesicularis, Pseudomonas syringae, Yersinia frederiksenii, Bacillus licheniformis, Enterobacter intermedius ve Serratia marcescens olarak tanımlanmış ve karakterize edilmiştir. Sonuç olarak, bu bakteri izolatları çeşitli ortamlarda deltametrinin parçaalanması için kullanılabilir.

Anahtar Kelimeler

• Bakteriler
• Biyodegradasyon
• Deltametrin
• İzolasyon

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