Determination of Antioxidant Properties of Ginkgo biloba in Different Forms

Abstract

Ginkgo biloba is a commercially an important plant used in both traditional and modern medicine. It is offered for sale in 3 different forms as drug, food supplement and leaf in in the world and our country. In this study, antioxidant properties were investigated by extracting sample in all 3 forms in acidic and acid-free environment. The total antioxidant capacity and total phenolic content of extracts were investigated by ABTS and Folin-Ciocalteu methods, respectively. In addition, the antioxidant substances were determined by HPLC (high performance liquid chromatography) analysis. It was determined that the antioxidant substance that Ginkgo biloba plant contains in the highest amount is rutin, which is a flavonoid glycoside. As a result of the study, it has been demonstrated that the product in drug form has better antioxidant properties than products in food supplement and leaf form.

Keywords

• Antioxidant
• Ginkgo biloba
• food supplement
• HPLC

Farklı Formlardaki Ginkgo biloba’nın Antioksidan Özelliklerinin Belirlenmesi

Öz

Ginkgo biloba hem geleneksel hem de modern tıpta kullanılan ticari olarak önemli bir bitkidir. Dünyada ve ülkemizde ilaç, gıda takviyesi ve yaprak olmak üzere 3 farklı formda satışa sunulmaktadır. Bu çalışmada her 3 formdaki numunenin asitli ve asitsiz ortamda ultrasonik ekstraksiyonu gerçekleştirilerek antioksidan özellikleri incelenmiştir. Ekstraktların antioksidan kapasitesi ve toplam fenolik madde içeriği sırasıyla ABTS ve Folin-Ciocalteu yöntemleri ile incelenmiştir. Ayrıca HPLC analizleri de yapılarak içerdikleri antioksidan maddeler tespit edilmiştir. Ginkgo biloba bitkisinin en yüksek miktarda içerdiği antioksidan maddenin bir flavonoid glikozit olan rutin olduğu belirlenmiştir. Çalışmanın sonucunda, ilaç formundaki ürünün gıda takviyesi ve yaprak formundaki ürünlerden daha iyi antioksidan özelliklere sahip olduğu ortaya konulmuştur.

Anahtar Kelimeler

• Antioksidan
• Ginkgo biloba
• gıda takviyesi

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