Deterjan Esaslı Hücresizleştirilen Tavuk Derisinin Doku İskelesi Olarak Karakterizasyonu

Öz

Özellikle son yıllarda doku mühendisliği uygulamaları için biyomalzemeler elde etmek amacıyla birçok doku ve organa hücresizleştirme yöntemi uygulanmıştır. Bu çalışmada, potansiyel bir doku iskelesi olarak hücresizleştirilmiş tavuk derisinin hazırlanması ve karakterizasyonu ile ilgili kapsamlı ve ayrıntılı incelemeler gerçekleştirilmiştir. Hücresizleştirme ajanı olarak kullanılan sodyum deoksikolat farklı zaman aralıklarında dokulara uygulanarak hücresizleştirme sürecinde en uygun yöntemin bulunabilmesi amacıyla DNA miktar tayini, hücresel bileşenlerin uzaklaştırılması, mekanik özellikler, ekstraselüler matriksin (ESM) korunması ve hücre canlılığı ile ilgili analizler yapılmıştır. Elde edilen sonuçlar, hücresizleştirme süresinin uzatılmasının hücresel bileşenlerin uzaklaştırılması ve dokudan uzaklaştırılan DNA miktarı yönünden avantaj sağlarken diğer taraftan ESM’nin korunması, mekanik özellikler ve hücre canlılığı yönünden dezavantaja sebep olduğunu göstermiştir. Bununla birlikte hücresizleştirilmiş tavuk derisinin, hücre tutunması, büyümesi ve proliferasyonunu destekleyen özellikte olduğu gösterilerek hazırlanan malzemenin biyouyumlu olduğu kanıtlanmıştır. Sonuç olarak, bu çalışmada hücresizleştirilmiş tavuk derisinin kolayca bulunabilen, ucuz ve biyouyumlu bir malzeme olarak, özellikle deri dokusu mühendisliği olmak üzere doku mühendisliği ile ilgili daha ileri çalışmalar için doku iskelesi hazırlanması amacıyla kullanılabilecek uygun bir biyomalzeme olduğu gösterilmiştir.

Anahtar Kelimeler

• doku mühendisliği
• hücresizleştirme
• tavuk derisi
• doku iskelesi
• biyomalzeme

Characterization of Detergent-based Decellularized Chicken Skin as a Tissue Scaffold

Abstract

Especially in recent years, decellularization method has been applied to many tissues and organs in order to obtain biomaterials for tissue engineering applications. In this study, comprehensive and detailed investigations were carried out on preparation and characterization of decellularized chicken skin as a potential tissue scaffold. Sodium deoxycholate was applied to tissues as a decellularization agent at different time intervals and analysis were performed on DNA quantitation, removal of cellular components, mechanical properties, preserving the extracellular matrix (ECM) and cell viability to find the most appropriate method in the process of decellularization. The results showed that extending the duration of decellularization provides advantages in terms of the removal of cellular components and the amount of DNA removed from the tissue, on the other hand, it causes disadvantages in terms of protection of ECM, mechanical properties, and cell viability. However, the prepared material was proven to be biocompatible by showing that decellularized chicken skin is capable of supporting cell attachment, growth, and proliferation. In conclusion, in this study, it was shown that decellularized chicken skin is an easily available, inexpensive, and biocompatible material that can be used for preparation of tissue scaffold for further studies in tissue engineering, especially in skin tissue engineering.

Keywords

• tissue engineering
• decellularization
• chicken skin
• tissue scaffold
• biomaterial

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