Gentamisin Konjuge Selüloz Nanokristallerinin Hazırlanması ve Farklı Mikroorganizmalar Üzerindeki Etkinliğinin Değerlendirilmesi

Öz

Tekstil üretiminde ortaya çıkan büyük miktarlardaki atık pamuk malzemesi, çevre sorunlarına ve kaynakların tükenmesine neden olmaktadır. Atık pamuğun geri dönüştürülmesinin en etkili yollarından biri nanokristalin selüloz üretimidir. Selüloz nanokristaller, çeşitli özelliklerinden dolayı biyomedikal uygulamalarda büyük ilgi görmektedir. Bu çalışmada atık pamuktan asit hidrolizi ile selüloz nanokristaller tek aşamada üretilmiştir. Selüloz ekstraksiyonu, atık pamuktan alkali muamelesi ile gerçekleştirildikten sonra selüloz nanokristaller, nitrik asit (%68 w/w) ve hidroklorür asit (%37 w/w) karışımı asit hidroliz metodu ile izole edilmiştir. Selüloz nanokristaller, sodyum periyodat (NaIO4) ile oksitlenerek modifiye edilmiştir. Hazırlanan selüloz nanokristaller, Transmisyon elektron mikroskobu (TEM) ve Fourier dönüşümlü kızılötesi spektroskopisi (FTIR) ile karakterize edildikten sonra gentamisin konjugasyon çalışmaları yapılmıştır. Gentamisin konjuge selüloz nanopartiküllerin, Escherichia coli (E. Coli), Staphylococcus aureus (S. aureus), Pseudomonas aeruginosa (P. aeruginosa), Klebsiella pneumoniae (K. pneumoniae)'ye karşı antimikrobiyal aktiviteleri ve Candida Albicans'a (C. Albicans) karşı antifungal etkisi araştırılmıştır. Sonuçlar, periyodat oksitlenmiş selüloz nanokristallerinin bakteri ve mantar üzerinde etkili olduğunu doğrulamıştır.

Anahtar Kelimeler

• Waste cotton
• cellulose nanocrystal
• mixed acid hydrolysis method
• gentamicin
• antimicrobial activity.

Preparation of Gentamicin Conjugated Cellulose Nanocrystals and Evaluation of Efficacy on Different Microorganisms

Abstract

Abstract Large amounts of waste cotton material generated in textile production cause environmental problems and resource depletion. The production of nanocrystalline cellulose is one of the effective way to the recycling of waste cotton. Cellulose nanoparticles are of great interest in biomedical applications due to their various properties. In this study cellulose nanocrystals were produced from waste cotton through acid hydrolysis in a single step. The extraction of cellulose from waste cotton was carried out by alkalin treatment, afterthat the cellulose nanocrystals were isolated by the nitric acid (68% w/w) and hydrochloride acid (37% w/w) mixture acid hydrolysis. Cellulose nanocrystals were oxidized by sodium periodate (NaIO4) for functionalization. The prepared cellulose nanocrystals were characterized by Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR) then gentamicin conjugation studies were carried out. Gentamicin conjugated cellulose nanoparticles were examined for antimicrobial activity against Escherichia coli (E. coli), Staphylococcus aureus (S. Aureus), Pseudomonas aeruginosa(aeruginosa), Klebsiella pneumoniae (K. pneumoniae) and antifungal activity against Candida albicans (C. Albicans). The results confirmed that the periodate oxidized cellulose nanocrystals are effective on bacterai and fungus.

Keywords

• Waste cotton
• cellulose nanocrystal
• mixed acid hydrolysis method
• gentamicin
• antimicrobial activity.

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