Aydın ve Denizli’deki Sıcak Su Kaynaklarından İzole EdilenTermofilik Bakterilerin Lipaz, Amilaz ve Proteaz Aktivitelerinin Araştırılması

Abstract

Termofilik bakteriler sıcak çevrelerde gelişebilen mikroorganizmalardır. Bu bakterilerin ürettikleri enzimler bitki ve hayvan kaynaklı enzimlere göre daha stabil olabilmekte ve daha ucuz olarak elde edilmektedirler.Termostabil enzimler ekstrem şartlarda esneklik ve tolerans göstermektedirler. Lipazlar biyoteknoloji ile ilişkili önemli gruplardır ve gıda, mandıra, deterjan ve ilaç endüstrisinde kullanılmaktadırlar. Diğer önemli bir enzim grubu olan amilazlar kağıt, şeker, tekstil, deterjan, fırında pişirme ve demleme endüstrisi gibi çeşitli alanlarda kullanılmaktadır. Proteazlar hidrolitik enzimlerdir ve deterjan üretimi, deri ürünleri, ilaç, et yumuşatıcı olarak, gıda ürünleri ve katı atık arıtma sanayii gibi çeşitli endüstriyel işlemlerde kullanılmaktadır. Bu çalışmada, Aydın ve Denizli’deki bazı termal alanlardan elde edilen 30 suşun 16S rDNA bölgeleri PCR ile çoğaltılmış ve moleküler tanıları yapılmıştır. Otuz izolat arasında suşların %50’si lipaz, %63.3’ü amilaz, %56.6’sı proteaz enzimlerini üretmektedirler. Lipaz-proteaz üreten üç, amilaz-proteaz üreten yedi izolat saptanırken, her üç enzimi de üreten 6 izolat olduğu belirlenmiştir.Sonuç olarak, çalışmamızda elde edilen suşlar ve ürettiklerienzimler endüstriyel biyoteknolojide kullanılma potansiyeline sahiptir.

Keywords

• Termofilik Bakteriler
• Lipaz
• Amilaz
• Proteaz
• 16S rDNA

Lipase, Amylase and Protease Activities Of Thermophilic Bacteria Isolated From Hot Springs In Aydin And Denizli

Abstract

Thermophilic microorganisms are able to grow at high temperatures. Enzymes produced by these kind of bacteria are more stable and cost effective. Thermostable enzymes have flexibility and tolerance in extreme conditions. Lipases dealing with biotechnology are important groups of enzymes. These enzymes are used in food, dairy, detergent and pharmaceutical industry. Amylases that are also important groups are used in various biotechnological fields such as paper, sugar, textile, detergent, baking and infusion industry. Proteases are hydrolytic enzymes and they are used in various industries such as producing detergent, leather products, pharmaceutical, meat tenderizer, food products and solid waste treatment industry. In this study, 16S rDNA regions of thirty bacterial strains isolated from thermal areas in Aydin and Denizli are amplified by PCR and isolates were identified by molecular methods. Among 30 isolates, percentage of strains that produce one enzyme is that; lipase %50, amylase %63.3, protease %56.6. Three of strains were found to be produce both Lipase and protease, while seven strains were amylase and protease producers. Six strains were found to capable of producing all three enzymes. As a result, isolates obtained in our study and their enzymes have an important potential in industrial biotechnology.

Keywords

• Thermophilic Bacteria
• Lipase
• Amylase
• Protease
• 16S rDNA

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