Year 2020, Volume , Issue 19, Pages 435 - 441 2020-08-31

Filamentous Fungi Associated with the Golot Cheese of the Rize-Ardesen Highlands in Turkey
Türkiye'de Rize-Ardeşen Yaylalarının Golot Peyniri ile İlişkili Filamentli Funguslar

Banu METİN [1]


The aim of this study was to identify using molecular methods the filamentous fungi associated with the Golot cheese produced in Ardesen, Rize Province, East Black Sea region, Turkey. Sixteen fungi were isolated from seven cheese samples and subjected to sequencing analysis using different fungal DNA barcodes. The first marker used, internal transcribed spacer (ITS), allowed identification of Geotrichum candidum and Penicillium roqueforti. The other isolates, defined as Penicillium and Cladosporium, could be identified only at the genus level using ITS; therefore, the beta-tubulin (benA) gene and the microsatellite PC4 markers were used to define the remaining Penicillium isolates as P. biforme and P. solitum. The Cladosporium isolate was identified as C. macrocarpum by analysis using benA and translation elongation factor 1-α (tef1) genes. The most common species associated with Golot cheeses were determined to be P. biforme (56%) and G. candidum (25%). Previously unknown sequences of P. solitum PC4 and C. macrocarpum tef1, and a longer version of C. macrocarpum benA were submitted to GenBank.
Bu çalışmanın amacı, Doğu Karadeniz Bölgesi, Rize İli, Ardeşen'de üretilen Golot peyniri ile ilişkili filamentli fungusların moleküler yöntemler kullanılarak belirlenmesidir. Yedi peynir örneğinden on altı fungus izole edilmiş ve farklı fungal DNA barkodları kullanılarak dizilim belirleme analizine tabi tutulmuştur. Kullanılan ilk markör olan internal transcribed spacer bölgesi (ITS), Geotrichum candidum ve Penicillium roqueforti'nin identifikasyonuna olanak vermiştir. Diğer izolatlar, ITS kullanılarak sadece cins düzeyinde Penicillium ve Cladosporium olarak tanımlanabilmiştir; bu nedenle, beta-tubulin (benA) geni ve mikrosatellit PC4 markörleri, kalan Penicillium izolatlarını P. biforme ve P. solitum olarak tanımlamak için kullanılmıştır. Cladosporium izolatı, benA ve translation elongation factor 1-α (tef1) genleri kullanılarak analiz edilerek C. macrocarpum olarak tanımlanmıştır. Golot peynirleriyle ilişkili en yaygın türlerin P. biforme (%56) ve G. candidum (%25) olduğu belirlenmiştir. Daha önce bilinmeyen P. solitum PC4 ve C. macrocarpum tef1 dizilimleri ve daha uzun bir C. macrocarpum benA versiyonu GenBank'a sunulmuştur.
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Primary Language en
Subjects Engineering
Journal Section Articles
Authors

Orcid: 0000-0002-3203-0058
Author: Banu METİN (Primary Author)
Institution: İSTANBUL SABAHATTİN ZAİM ÜNİVERSİTESİ
Country: Turkey


Supporting Institution The Scientific and Technological Research Council of Turkey (TUBITAK) to B. M.
Project Number Grant no. 119O314
Thanks The author thanks Havva Balcan for providing the cheese samples.
Dates

Publication Date : August 31, 2020

APA Meti̇n, B . (2020). Filamentous Fungi Associated with the Golot Cheese of the Rize-Ardesen Highlands in Turkey . Avrupa Bilim ve Teknoloji Dergisi , (19) , 435-441 . DOI: 10.31590/ejosat.715639