Control of Fusarium oxysporum f. sp. radicis lycopersici Jarvis & Shoemaker (Ascomycota: Hypocreales) and Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Nemata: Meloidogynidae) with Aspergillus niger Tiegh. (Ascomycota: Eurotiales) culture filtrate on tomato

Year 2023, , 123 - 132, 20.07.2023

Fatma Gül Göze Özdemir , Şerife Evrim Arıcı

https://doi.org/10.16970/entoted.1240155

Abstract

The effects of Aspergillus niger Tiegh. (Ascomycota: Eurotiales) culture filtrate on Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (N) and Fusarium oxysporum f. sp. radicis lycopersici Jarvis & Shoemaker (Ascomycota: Hypocreales) (FORL) were investigated under controlled conditions on tomato between April and August 2022 in the Isparta province. The study consisted of 8 treatments; 1: N, 2: FORL, 3: N+A. niger, 4: FORL+A. niger, 5: N+FORL, 6: N+FORL+A. niger, 7: N+FORL+nematicide, 8: N+FORL+fungicide. In inoculation, 1000 M. incognita second juvenile larvae/1ml and 3X106 spore/ml FORL were used for each seedling according to treatment. Two days after inoculation, 10 ml of undiluted A. niger culture filtrate was applied to each potting soil. After 60 days, 0-9 gall and egg mass index, and 0-4 disease severity scale were evaluated. While the suppressive effect of A. niger culture filtrate on the gall and egg mass of M. incognita was found over 55%, disease severity was found to be over 25%. The highest suppressive effect on gall and egg mass was determined in N+FORL+nematicide, followed by N+FORL+A. niger. The disease severity of N+FORL+A. niger, N+FORL+nematicide, and N+FORL+fungicide has been determined to be lower than N+FORL and FORL.

Keywords

Aspergillus niger, fermentation fluid, fungicidal effect, Fusarium wilt, nematicidal effect

Supporting Institution

X

Project Number

X

Thanks

Part of this study presented as a poster presentation at 8th International Entomopathogens and Microbial Control Congress, October 06th-08th, 2022- Antalya, Türkiye. In addition, Olympos Seedling Production Facility and Agricultural Engineer Tuğçe Okumuş Erol are thanked for providing tomato seedlings.

Domateste Fusarium oxysporum f. sp radicis lycopersici (Jarvis & Shoemaker) (Ascomycota: Hypocreales) ve Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (Nemata: Meloidogynidae)’nın Aspergillus niger Tiegh. (Ascomycota: Eurotiales) kültür filtratı ile kontrolü

Abstract

Aspergillus niger Tiegh. (Ascomycota: Eurotiales) kültür filtratının Meloidogyne incognita (Kofoid & White, 1919) Chitwood, 1949 (N) ve Fusarium oxysporum f. sp. radicis lycopersici Jarvis & Shoemaker (Ascomycota: Hypocreales) (FORL) üzerindeki etkileri Isparta ilinde Nisan-Ağustos 2022 tarihleri arasında domateste kontrollü koşullarda araştırılmıştır. Çalışma 8 uygulamadan oluşmaktadır; 1: N, 2: FORL, 3: N+A. niger, 4: FORL+A. niger, 5: N+FORL, 6: N+FORL+A. niger, 7: N+FORL+nematisit, 8: N+FORL+fungusit. İnokulasyonda her fide için uygulamaya göre 1000 M. incognita ikinci dönem larva/1ml ve 3X106 spor/ml FORL kullanılmıştır. İnokulasyondan iki gün sonra, her saksı toprağına 10 ml seyreltilmemiş A. niger kültür filtratı uygulanmıştır. Altmış gün sonra 0-9 gal ve yumurta paketi indeksi ve 0-4 hastalık şiddeti skalası değerlendirilmiştir. Aspergillus niger kültür filtratı uygulamasının M. incognita'nın gal ve yumurta paketi üzerindeki baskılayıcı etkisi %55'in üzerinde bulunurken, hastalık şiddeti üzerindeki baskılayıcı etkisi %25'in üzerinde bulunmuştur. Gal ve yumurta paketi üzerindeki baskılayıcı etki en yüksek N+FORL+nematisit’de belirlenmiştir, ardından N+FORL+A. niger uygulamasının geldiği belirlenmiştir. N+FORL+A. niger, N+FORL+nemasit ve N+FORL+fungisit uygulamalarının hastalık şiddeti N+FORL ve FORL uygulamalarına göre daha düşük saptanmıştır.

Keywords

Aspergillus niger, fermente sıvı, fungisidal etki, Fusarium solgunluğu, nematisidal etki

Project Number

X

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