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A study of whether the genetic variation decreased or not in the protected Caucasian bee, Apis mellifera caucasica Pollmann, 1889 (Hymenoptera: Apidae) population in isolated regions

Year 2023, , 271 - 282, 09.10.2023

Berkant İsmail Yıldız , Emel Tüten , Sinan Aydın , Yaprak Karaduman Aslan , Ramazan Çetin , Erdem Sur , Kemal Karabağ

https://doi.org/10.16970/entoted.1273612

Abstract

The Caucasian honeybee, Apis mellifera caucasica Pollmann, 1889 (Hymenoptera: Apidae), is one of the most productive bee subspecies. This subspecies, which has special importance for Türkiye, has been taken under protection in two isolated regions (Artvin and Ardahan) since 2000. To date, no study has been conducted on whether genetic diversity has decreased in these protected Caucasian honeybee colonies. Therefore in 2022, worker bees were collected from 100 different colonies in 15 different locations in these two regions and their genetic variations were examined using 30 microsatellite loci. The average number of alleles per locus was 13.57, and the loci had a high level of information content according to the PIC (0.7) value. FIS (0.96) and FST (0.01) values showed low genetic diversity and high inbreeding in populations. Genetic variations were calculated as 0.77% among populations, 99.23% among individuals in populations, and 0% among all individuals. Also, populations deviated from the Hardy-Weinberg equilibrium (p<0.001). Significant bottleneck evidence was found for Artvin in the analysis results using the two-phase mutation model. These results provide important information that can be used as a guide for Caucasian bee breeding strategies and conservation programs.

Keywords

Apis mellifera Caucasian bee genetic variation microsatellite population structure

Supporting Institution

Türkiye Cumhuriyeti Tarım ve Orman Bakanlığı

Project Number

2021A03-168656

Thanks

We thank The Republic of Turkey Ministry of Agriculture. The project was supported by HAYGEM with project number 2021A03-168656.

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İzole bölgelerde korunan Kafkas arısı, Apis mellifera caucasica Pollmann, 1889 (Hymenoptera: Apidae) popülasyonunda genetik varyasyonun azalıp azalmadığına dair bir çalışma

Year 2023, , 271 - 282, 09.10.2023

Berkant İsmail Yıldız , Emel Tüten , Sinan Aydın , Yaprak Karaduman Aslan , Ramazan Çetin , Erdem Sur , Kemal Karabağ

https://doi.org/10.16970/entoted.1273612

Abstract

Kafkas arısı, Apis mellifera caucasica Pollmann, 1889 (Hymenoptera: Apidae), en verimli arı alt türlerinden biridir. Türkiye için özel bir öneme sahip olan bu alt tür, 2000 yılından itibaren iki izole bölgede (Artvin ve Ardahan) koruma altına alınmıştır. Bugüne kadar korunan Kafkas bal arısı kolonilerinde genetik çeşitliliğin azalıp azalmadığına dair bir çalışmaya rastlanmamıştır. Bu nedenle 2022 yılında, bu iki izole bölgede 15 farklı lokasyonda bulunan 100 farklı koloniden işçi arılar toplanmış ve 30 mikrosatelit lokusu kullanılarak genetik varyasyonlar incelenmiştir. Lokus başına ortalama allel sayısı 13.57 bulunmuştur ve PIC (0.7) değerine göre lokuslar yüksek düzeyde bilgi içeriğine sahiptir. FIS (0.96) ve FST (0.01) değerleri popülasyonlarda düşük genetik çeşitlilik ve yüksek akrabalı yetiştirme olduğunu göstermiştir. Genetik varyasyonlar, popülasyonlar arasında %0.77, popülasyonlardaki bireyler arasında %99.23 ve tüm bireyler arasında %0 olarak hesaplanmıştır. Ayrıca, popülasyonlar Hardy-Weinberg dengesinden sapmıştır (P<0.001). İki fazlı mutasyon modeli kullanılarak yapılan analiz sonuçlarında Artvin için önemli bir darboğaz kanıtı bulunmuştur. Bu sonuçlar, Kafkas arısı ıslah stratejileri ve koruma programları için kılavuz olarak kullanılabilecek önemli bilgiler sağlamaktadır.

Keywords

Apis mellifera kafkas arısı genetik varyasyon mikrosatelit popülasyon yapısı

Project Number

2021A03-168656

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  • Hassett, J., K. A. Browne, G. P. McCormack, E. Moore, N. I. H. B. Society, G. Soland & M. Geary, 2018. A significant pure population of the dark European honey bee (Apis mellifera mellifera) remains in Ireland. Journal of Apicultural Research, 57 (3): 337-350.
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  • Kandemir, I. & A. Kence, 1995. Allozyme variability in a central Anatolian honey bee (Apis mellifera L.) population. Apidologie, 26 (6): 503-510.
  • Kandemir, I., M. Kence & A. Kence, 2000. Genetic and morphometric variation in honeybee (Apis mellifera L.) populations of Turkey. Apidologie, 31 (3): 343-356.
  • Kandemir, I., M. Kence, W. S. Sheppard & A. Kence, 2006. Mitochondrial DNA variation in honey bee (Apis mellifera L.) populations from Turkey. Journal of Apicultural Research, 45 (1): 33-38.
  • Karabağ, K., R. I. Tunca, E. Tüten & T. Doğaroğlu, 2020. Current genetic status of honey bees in Anatolia in terms of thirty polymorphic microsatellite markers. Turkish Journal of Entomology, 44 (3): 333-346.
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There are 62 citations in total.

Details

Primary Language English
Subjects Entomology
Journal Section Articles
Authors

Berkant İsmail Yıldız 0000-0001-8965-6361

Emel Tüten 0000-0002-3105-6416

Sinan Aydın 0000-0002-7234-5804

Yaprak Karaduman Aslan 0000-0002-8778-7699

Ramazan Çetin 0000-0002-3640-8185

Erdem Sur 0000-0002-9383-7146

Kemal Karabağ 0000-0002-4516-6480

Project Number 2021A03-168656
Early Pub Date October 7, 2023
Publication Date October 9, 2023
Submission Date March 30, 2023
Acceptance Date September 25, 2023
Published in Issue Year 2023

Cite

APA Yıldız, B. İ., Tüten, E., Aydın, S., Karaduman Aslan, Y., et al. (2023). A study of whether the genetic variation decreased or not in the protected Caucasian bee, Apis mellifera caucasica Pollmann, 1889 (Hymenoptera: Apidae) population in isolated regions. Turkish Journal of Entomology, 47(3), 271-282. https://doi.org/10.16970/entoted.1273612
AMA Yıldız Bİ, Tüten E, Aydın S, Karaduman Aslan Y, Çetin R, Sur E, Karabağ K. A study of whether the genetic variation decreased or not in the protected Caucasian bee, Apis mellifera caucasica Pollmann, 1889 (Hymenoptera: Apidae) population in isolated regions. TED. October 2023;47(3):271-282. doi:10.16970/entoted.1273612
Chicago Yıldız, Berkant İsmail, Emel Tüten, Sinan Aydın, Yaprak Karaduman Aslan, Ramazan Çetin, Erdem Sur, and Kemal Karabağ. “A Study of Whether the Genetic Variation Decreased or Not in the Protected Caucasian Bee, Apis Mellifera Caucasica Pollmann, 1889 (Hymenoptera: Apidae) Population in Isolated Regions”. Turkish Journal of Entomology 47, no. 3 (October 2023): 271-82. https://doi.org/10.16970/entoted.1273612.
EndNote Yıldız Bİ, Tüten E, Aydın S, Karaduman Aslan Y, Çetin R, Sur E, Karabağ K (October 1, 2023) A study of whether the genetic variation decreased or not in the protected Caucasian bee, Apis mellifera caucasica Pollmann, 1889 (Hymenoptera: Apidae) population in isolated regions. Turkish Journal of Entomology 47 3 271–282.
IEEE B. İ. Yıldız, E. Tüten, S. Aydın, Y. Karaduman Aslan, R. Çetin, E. Sur, and K. Karabağ, “A study of whether the genetic variation decreased or not in the protected Caucasian bee, Apis mellifera caucasica Pollmann, 1889 (Hymenoptera: Apidae) population in isolated regions”, TED, vol. 47, no. 3, pp. 271–282, 2023, doi: 10.16970/entoted.1273612.
ISNAD Yıldız, Berkant İsmail et al. “A Study of Whether the Genetic Variation Decreased or Not in the Protected Caucasian Bee, Apis Mellifera Caucasica Pollmann, 1889 (Hymenoptera: Apidae) Population in Isolated Regions”. Turkish Journal of Entomology 47/3 (October 2023), 271-282. https://doi.org/10.16970/entoted.1273612.
JAMA Yıldız Bİ, Tüten E, Aydın S, Karaduman Aslan Y, Çetin R, Sur E, Karabağ K. A study of whether the genetic variation decreased or not in the protected Caucasian bee, Apis mellifera caucasica Pollmann, 1889 (Hymenoptera: Apidae) population in isolated regions. TED. 2023;47:271–282.
MLA Yıldız, Berkant İsmail et al. “A Study of Whether the Genetic Variation Decreased or Not in the Protected Caucasian Bee, Apis Mellifera Caucasica Pollmann, 1889 (Hymenoptera: Apidae) Population in Isolated Regions”. Turkish Journal of Entomology, vol. 47, no. 3, 2023, pp. 271-82, doi:10.16970/entoted.1273612.
Vancouver Yıldız Bİ, Tüten E, Aydın S, Karaduman Aslan Y, Çetin R, Sur E, Karabağ K. A study of whether the genetic variation decreased or not in the protected Caucasian bee, Apis mellifera caucasica Pollmann, 1889 (Hymenoptera: Apidae) population in isolated regions. TED. 2023;47(3):271-82.