Environmental persistence of the conidia of native entomopathogenic fungi and their efficiency on Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae)

Year 2024, , 327 - 342, 16.10.2024

Mehmet Sedat Sevinç , İsmail Karaca

https://doi.org/10.16970/entoted.1498947

Abstract

This study aimed to investigate the effects of local Beauveria bassiana (Balsamo) Vuillemin and Cordyceps fumosorosea (Wize) (Hypocreales: Cordycipitaceae) isolates on Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae) prepupae, during 2020 and 2021 in the Mediterranean Region of Türkiye. In petri dishes, mortality rates varied with doses: for LD.2016 (B. bassiana), between 30 and 60%, for M6-4 (B. bassiana), 30-46%, of IFR (C. fumosorosea), 65-100%. Additionally, differences were detected between the prepupa and pupa periods of the larvae depending on the isolate. In sterile soil studies, the IFR caused mortality rates of 53-93%, while those for the LD.2016 isolate were 20-55%, depending on the doses. In conidial survival studies, the IFR caused mortality rates of 70-93% at 0 months and 44-60% in the 12th month. The LD.2016 mortality was among 37-55% at 0 months, while mortality rates decreased to 2% in the 12th month. The reproductive capacity of adult individuals that emerged alive after infection was negatively affected depending on time and fungal isolate. In semi-controlled field conditions, the IFR isolate had mortality rates varied between 40 and 65%, and LD.2016 mortality rates varied between 34 and 62%, depending on doses. It was observed that the fungi used in the study had potential in biological control of C. capitata.

Keywords

Beauveria bassiana, Cordyceps fumosorosea, reproductive capacity, soil surface application, sublethal effects

Supporting Institution

Ministry of Agriculture and Forestry of the Republic of Turkey, General Directorate of Agricultural Research and Policies

Project Number

TAGEM/BSAD/A/21/A2/P5/2404

Thanks

This study was funded by the Ministry of Agriculture and Forestry of the Republic of Turkey, General Directorate of Agricultural Research and Policies, (with the project number TAGEM/BSAD/A/21/A2/P5/2404), and the manuscript was produced from a part of data from first author’s PhD. dissertation. Petri dishes bioassay results and the first application results of conidial persistence studies (0th month on sterile soil bioassays), which form a part of this study, were presented as an oral presentation at the 8th International Entomopathogens and Microbial Control Congress held in Antalya-Turkey on 6-8 October 2022, and the summary was included in the congress summary book.

Yerel entomopatojen fungus konidialarının çevresel kalıcılığı ve Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae) üzerindeki etkinliği

Abstract

Bu çalışmada, 2020-2021 yıllarında Türkiye Akdeniz Bölgesi'nde yerel Beauveria bassiana (Balsamo) Vuillemin ve Cordyceps fumosorosea (Wize) (Hypocreales: Cordycipitaceae) izolatlarının Ceratitis capitata (Wiedemann, 1824) (Diptera: Tephritidae) prepupaları üzerindeki etkilerinin araştırılması amaçlanmıştır. Petri kaplarında ölüm oranları dozlara göre; LD.2016 (B. bassiana) için %30-60 arasında, M6-4 (B. bassiana) için %30-46 arasında, IFR (C. fumosorosea) için %65-100 arasında değişmiştir. Ayrıca, izolatlara bağlı olarak larvaların prepupa ve pupa dönemleri arasında farklılıklar tespit edilmiştir. Steril toprak çalışmalarında IFR %53-93 arasında mortaliteye neden olurken, LD.2016 izolatı için bu oran doza bağlı olarak %20-55 olmuştur. Konidial hayatta kalma çalışmalarında IFR 0. ayda %70-93, 12. ayda ise %44-60 arasında mortaliteye neden olmuştur. LD.2016 mortalitesi 0. ayda %37-55 arasında iken, 12. ayda mortalite oranları %2'ye düşmüştür. Enfeksiyondan sonra canlı çıkan ergin bireylerin üreme kapasitesi zamana ve fungal izolata bağlı olarak olumsuz etkilenmiştir. Yarı kontrollü arazi koşullarında IFR izolatında mortalite oranları doza bağlı olarak %40-65 arasında, LD.2016 mortalite oranları ise %34-62 arasında değişmiştir. Çalışmada kullanılan fungusların C. capitata'nın biyolojik mücadelesinde potansiyel taşıdığı görülmüştür.

Keywords

Beauveria bassiana, Cordyceps fumosorosea, üreme kapasitesi, toprak yüzey uygulaması, ölümcül olmayan etkiler

Project Number

TAGEM/BSAD/A/21/A2/P5/2404

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