Effects of pyriproxyfen on bioenergetic resources of Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)

Abstract

Colorado potato beetles (CPB) overwinters as adults in the soil, thus, their survival is related with their energy reserves. The present study examined the biochemical changes in adults treated with sub-lethal concentrations (≤LC₃₀) of pyriproxyfen. First, both the plants and overwintered adults were sprayed with different levels of pyriproxyfen (0, 250, 500 and 750 µl/l) under field conditions and were tested at 3, 6 and 12 days after treatment. Next, the adults from the first experiments were re-sprayed 1 week later with pyriproxyfen at the same concentrations and were re-sampled at 3, 6 and 12 days after the second treatment. The lipid, sugar, glycogen and protein levels of 4 males and 4 females were determined (mg/g; w/w). Results revealed a significant decline in lipid (87.7%), glycogen (50%) and caloric contents (75%) levels with respect to controls. Sugar and protein levels increased 7.89 and 5.79 times with respect to the controls. The best results were obtained at 250 µl/l of pyriproxyfen. The second round of testing demonstrated the additive effects of pyriproxyfen on bioenergetic reserves. Only the protein level showed a significant difference by sex. 

Keywords

• Colorado potato beetle
• lipids
• carbohydrates
• proteins
• admiral
• pyriproxyfen

Pyriproxyfenin Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae)’nın biyoenerji kaynakları üzerine etkileri

Öz

Patates böceği, toprakta ergin olarak kışlamaktadır. Bu nedenle, onların hayatta kalmaları enerji rezervleri ile ilişkilidir. Bu çalışmada pyriproxyfenin alt öldürücü konsantrasyonları (≤LC₃₀) uygulanmış erginlerdeki biyokimyasal değişiklikler incelenmiştir. İlk olarak, bitki ve kışlaktaki erginlere tarla şartlarında pyriproxyfen (0, 250, 500 ve 750 µl/l) farklı düzeylerde püskürtülmüş ve uygulamadan 3, 6 ve 12 gün sonra test edilmiştir. Birinci deneyden alınan erginlere 1 hafta sonra tekrar aynı konsantrasyonlarda pyriproxyfen uygulanmış ve 3, 6 ve 12 gün sonunda yeniden örneklenmiştir. 4 erkek ve 4 dişi bireyde lipit, şeker, glikojen ve protein düzeyleri (mg/g; a/a) tespit edilmiştir. Sonuçlar, kontrole göre lipid (% 87.7), glikojen (% 50) ve kalori içeriği (% 75) düzeylerinde önemli bir düşüş göstermiştir. Şeker ve protein düzeyleri kontrole göre 7.89 ve 5.79 kat artmıştır. En iyi sonuçlar, pyriproxyfenin 250 µl/l dozunda elde edilmiştir. Testin ikinci turu pyriproxyfenin biyoenerjik rezervler üzerindeki ilave etkilerini göstermiştir. Sadece protein düzeyi cinsiyete göre anlamlı bir farklılık göstermiştir.

Anahtar Kelimeler

• Patates böceği
• karbonhidratlar
• proteinler
• admiral
• pyriproxyfen

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