MikroRNA Ekspresyon Profillemesinde Yaygın Kullanılan Normalizasyon Yaklaşımları

Abstract

MikroRNA (miRNA) ekspresyonlarının belirlenmesinde RT-qPCR, mikroarray platformları ve miRNA dizileme en yaygın kullanılan tekniklerdir. Tüm bu tekniklerin kullanıldığı çalışmalarda en önemli hususlardan biri verilerin uygun normalizasyon yöntemi ile normalize edilmesidir. Normalizasyon ile biyolojik ve teknik varyasyonların sonuçlar üzerine olan etkisinin elimine edilmesi amaçlanmaktadır. MiRNA ekspresyonu çalışmalarında, farklı tekniklerden elde edilen verilerin normalizasyonunda kullanılan çok sayıda normalizasyon yaklaşımı kullanılmaktadır. Bu derlemede, miRNA ekspresyonu çalışmalarında en yaygın kullanılan normalizasyon yaklaşımları hakkında bilgiler özetlenmiştir.

Keywords

• Mikroarray
• mikroRNA
• miRNA dizileme
• normalizasyon
• RT-qPCR

Commonly Used Normalization Approaches in MicroRNA Expression Profiling

Abstract

RT-qPCR, microarray platforms and miRNA sequencing are the most common techniques used to determine microRNA (miRNA) expressions. One of the most important issues in studies these techniques are used is the normalization of the data by using appropriate normalization method. The purpose of normalization is to eliminate the effects of biological and technical variations on study results. Numerous normalization approaches are used for normalization of data obtained from different techniques in miRNA expression studies. In this review, information about the most commonly used normalization approaches in miRNA expression studies is summarized.

Keywords

• Microarray
• microRNA
• miRNA sequencing
• normalization
• RT-qPCR

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