The Effects of Baicalin on Lipid peroxidation and Oxidative Damage in Rats Exposed to Cypermethrine

Abstract

This study aims to investigate the protective effects of baicalin (BAI) in rats exposed to cypermethrin (CYP). A total of 40 Wistar Albino male rats, 10 in each group, aged 6 to 8 weeks and weighing 180 to 220 g, were utilized for this experiment. The groups were determined, respectively, control, BAI (50 mg kg-1), CYP (25 mg kg-1), and CYP (25 mg kg-1) + BAI (50 mg kg-1). At the end of the 21-day trial period, blood samples were collected from rats by cardiac puncture into test tubes with anticoagulant and non-anticoagulant properties under intraperitoneal ketamine and xylazine anesthesia. Afterward, the rats were sacrificed by cervical dislocation, and the liver, kidney, brain, testis, heart and lung tissues were removed. Glutathione, NO, and MDA levels and GPx, GR, GST, SOD, and CAT enzyme activities in tissue and blood (plasma and erythrocyte) samples were measured on a microplate reader by a spectrophotometric method, whereas LDH, AST, ALT, ALP, triglyceride, cholesterol, albumin, total protein, BUN, uric acid and creatinine levels/activities in serum were measured on a Roche Cobas autoanalyzer. At the end of the experiment, it was determined that the parameters of the BAI group were similar to those of the control group (P>0.05). When the CYP- exposed group was compared to the control group, it was discovered that there was a statistically considerable de- crease in tissue GSH levels as well as GPx, GR, GST, SOD, and CAT enzyme activities (P<0.05). A significant in- crease was recorded in MDA and NO levels (P<0.05). Additionally, while serum total protein and albumin levels de- creased, AST, ALT, ALP, LDH, cholesterol, triglyceride, BUN, uric acid, and creatinine levels/activities increased (P<0.05). The effects of CYP were partially or completely ameliorated in the CYP and BAI co-administered group. Consequently, it was revealed that BAI may have a protective effect against CYP-induced oxidative damage in rats by suppressing oxidative stress and promoting antioxidant system activity.

Keywords

• Baicalin
• cypermethrine
• lipid peroxidation
• oxidative stress
• pyrethroid

Sipermetrine Maruz Kalan Sıçanlarda Baikalinin Lipid Peroksidasyon ve Oksidatif Hasar Üzerine Etkileri

Abstract

Bu çalışmada sipermetrin (SPM) maruz kalan sıçanlarda baikalinin (BAİ) koruyucu etkilerinin araştırılması amaç- landı. Çalışmada her grupta 10 tane olacak şekilde toplam 40 adet 6-8 haftalık 180-220 g ağırlığında Wistar Albino ırkı erkek sıçan kullanıldı. Gruplar sırasıyla kontrol, BAİ (50 mg kg-1), SPM (25 mg kg-1) ve SPM (25 mg kg-1) + BAİ (50 mg kg-1) olarak belirlendi. 21 günlük deneme süresinin sonunda intraperitoneal ketamin-ksilazin anestezisi altında kalbe punksiyon yapılarak heparinize ve antikoagulansız özellikteki test tüplerine kan örneği alındı. Servikal dislokasyon son- rası sıçanlardan karaciğer, böbrek, beyin, testis, kalp ve akciğer dokuları çıkarıldı. Doku ve kan (plazma ve eritrosit) örneklerinde GSH, NO ve MDA düzeyleri ile GPx, GR, GST, SOD ve CAT enzim aktiviteleri mikroplaka okuyucuda spekrofotometrik yöntemle ölçülürken serumda LDH, AST, ALT ve ALP enzim aktiviteleri ile trigliserit, kolesterol, albü- min, total protein, BUN, ürik asit ve kreatinin düzeyleri Roche Cobas otoanalizöründe ölçüldü. Çalışma sonunda BAİ uygulanan grubun parametrelerinin kontrol grubuna benzer olduğu görüldü (P>0.05). Sipermetrin uygulanan grupta kontrole kıyasla doku GSH düzeyleri ile GPx, GR, GST, SOD ve CAT enzim aktivitelerinde anlamlı bir azalma tespit edildi (P<0.05). Malondialdehit ve NO seviyelerinde ise anlamlı bir artış kaydedildi (P<0.05). Ayrıca serum AST, ALT, ALP, LDH, kolesterol, trigliserit, BUN, ürik asit ve kreatinin düzey/aktivitelerinde artış görülürken serum total protein ve albümin düzeylerinde azalma görüldü (P<0.05). Sipermetrin ile BAİ’nin birlikte uygulandığı gruplarda SPM’nin etkileri kısmen ya da tamamen iyileştirdi. Sonuç olarak, BAİ'nin sıçanlarda oksidatif stresi baskılayarak ve antioksidan sistem aktivitesini artırarak SPM’nin neden olduğu oksidatif hasara karşı koruyucu etkiye sahip olabileceği ortaya konuldu.

Keywords

• Baikalin
• lipid peroksidasyon
• oksidatif stres
• piretroid
• sipermetrin

References

1. Afolabi OK, Aderibigbe FA, Folarin DT, Arinola A, Wusu AD. Oxidative stress and inflammation following sub-lethal oral exposure of cypermethrin in rats: Mitigating potential of epicatechin. Heliyon 2019; 5(8): e02274.
2. Arafa MH, Mohamed DA, Atteia HH. Ameliorative effect of N-acetyl cysteine on alpha-cypermethrin- induced pulmonary toxicity in male rats. Environ Toxicol 2015; 30(1): 26-43.
3. Carlberg I, Mannervik B. Glutathione reductase. Met- hods Enzymol 1985; 113: 484-90.
4. Chrustek A, Hołyńska-Iwan I, Dziembowska I, Bogu- siewicz J, Wróblewski M, Cwynar A, Olszewska- Słonina D. Current research on the safety of pyrethroids used as insecticides. Medicina (Kaunas) 2018; 54(4): 61.
5. Das T, Pradhan A, Paramanik A, Choudhury SM. Ameliorative role of zinc on cypermethrin-induced changes in haematological parameters and oxidative stress biomarkers in rat erythrocytes. Toxicol Environ Health Sci 2016; 8: 234-46.
6. de Oliveira MR, Nabavi SF, Habtemariam S, Erdogan Orhan I, Daglia M, Nabavi SM. The effects of baicalein and baicalin on mitochondrial function and dynamics: A review. Pharmacol Res 2015; 100: 296-308.
7. Dias MC, Pinto DCGA, Silva AMS. Plant flavonoids: chemical characteristics and biological activity. Molecules 2021; 26(17): 5377.
8. Ganguly R, Kumar R, Pandey AK. Baicalin provides protection against fluoxetine-induced hepatotoxicity by modulation of oxidative stress and inflammation. World J Hepatol 2022; 14(4): 729-43.

9. Glorennec P, Serrano T, Fravallo M, Warembourg C, Monfort C, Cordier S, Viel JF, Le Gléau F, Le Bot B, Chevrier C. Determinants of children's exposure to pyrethroid insecticides in Western France. Environ Int 2017; 104: 76-82.
10. Habig, WH, Pabst, MJ, Jakoby, WB. Glutahione s transferase. The first enzymatic steps in mercapturic acid formation, J Biol Chem 1957; 249: 7130- 9.
11. Ince S, Kucukkurt I, Demirel HH, Turkmen R, Sever E. Thymoquinone attenuates cypermethrin induced oxidative stress in Swiss albino mice. Pestic Biochem Physiol 2012; 104(3): 229-35.
12. Jabłońska-Trypuć A, Wiater J. Protective effect of plant compounds in pesticides toxicity. J Environ Health Sci Engineer 2022.
13. Liu LL, Gong LK, Wang H, Xiao Y, Wu XF, Zhang YH, Xue X, Qi XM, Ren J. Baicalin protects mouse from Concanavalin A-induced liver injury through inhibition of cytokine production and hepatocyte apoptosis. Liver Int 2007; 27(4): 582-91.
14. Lowry OH, Rosebrough NJ, Farr AL, Randal RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193: 265-75
15. Luck, H. Catalase. Bergmeyer, HU. eds. In: Method of Enzymatic Analysis. New York: Academic Press, 1965; pp. 885-94.
16. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95(2): 351-8.
17. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70: 158- 69.
18. Sankar P, Telang AG, Manimaran A. Protective effect of curcumin on cypermethrin-induced oxidative stress in Wistar rats. Exp Toxicol Pathol 2012; 64 (5): 487-93.
19. Sedlak J, Lindsay RH. Estimation of total, proteinbound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem 1968; 25(1): 192-205.
20. Shi J, Wu G, Zou X, Jiang K. Enteral baicalin, a flavone glycoside, reduces indicators of cardiac surgery associated acute kidney injury in rats. Cardiorenal Med 2019; 9(1): 31-40.
21. Sule RO, Condon L, Gomes AV. A common feature of pesticides: oxidative stress-the role of oxidative stress in pesticide-induced toxicity. Oxid Med Cell Longev 2022; 2022: 5563759.
22. Sun X, Wang X, He Q, Zhang M, Chu L, Zhao Y, Wu Y, Zhang J, Han X, Chu X, Wu Z, Guan S. Investigation of the ameliorative effects of baicalin against arsenic trioxide-induced cardiac toxicity in mice. Int Immunopharmacol 2021; 99: 108024.
23. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34(3): 497-500.
24. Tracey, WR, Tse J, Carter, G. Lipopolysaccharide- induced changes in plasma nitrite and nitrate concentrations in rats and mice: pharmacological evaluation of nitric oxide synthase inhibitors. JPET 1995; 272: 1011-5.
25. Wen YF, Zhao JQ, Bhadauria M, Nirala SK. Baicalin prevents cadmium induced hepatic cytotoxicity, oxidative stress and histomorphometric alterations. Exp Toxicol Pathol 2013; 65(1-2): 189-96.
26. Zeng X, Du Z, Ding X, Jiang W. Protective effects of dietary flavonoids against pesticide-induced toxicity: A review. Trends Food Sci Technol 2021; 109: 271-9.
27. Zhang Z, Gao X, Guo M, Jiang H, Cao Y, Zhang N. The protective effect of baicalin against lead- induced renal oxidative damage in mice. Biol Trace Elem Res 2017; 175(1): 129-35.