The Effects of Baicalin on Lipid peroxidation and Oxidative Damage in Rats Exposed to Cypermethrine

Öz

This study aims to investigate the protective effects of baicalin (BAI) in rats exposed to cypermethrin (CYP). A total of 40 Wistar Albino male rats, 10 in each group, aged 6 to 8 weeks and weighing 180 to 220 g, were utilized for this experiment. The groups were determined, respectively, control, BAI (50 mg kg-1), CYP (25 mg kg-1), and CYP (25 mg kg-1) + BAI (50 mg kg-1). At the end of the 21-day trial period, blood samples were collected from rats by cardiac puncture into test tubes with anticoagulant and non-anticoagulant properties under intraperitoneal ketamine and xylazine anesthesia. Afterward, the rats were sacrificed by cervical dislocation, and the liver, kidney, brain, testis, heart and lung tissues were removed. Glutathione, NO, and MDA levels and GPx, GR, GST, SOD, and CAT enzyme activities in tissue and blood (plasma and erythrocyte) samples were measured on a microplate reader by a spectrophotometric method, whereas LDH, AST, ALT, ALP, triglyceride, cholesterol, albumin, total protein, BUN, uric acid and creatinine levels/activities in serum were measured on a Roche Cobas autoanalyzer. At the end of the experiment, it was determined that the parameters of the BAI group were similar to those of the control group (P>0.05). When the CYP- exposed group was compared to the control group, it was discovered that there was a statistically considerable de- crease in tissue GSH levels as well as GPx, GR, GST, SOD, and CAT enzyme activities (P<0.05). A significant in- crease was recorded in MDA and NO levels (P<0.05). Additionally, while serum total protein and albumin levels de- creased, AST, ALT, ALP, LDH, cholesterol, triglyceride, BUN, uric acid, and creatinine levels/activities increased (P<0.05). The effects of CYP were partially or completely ameliorated in the CYP and BAI co-administered group. Consequently, it was revealed that BAI may have a protective effect against CYP-induced oxidative damage in rats by suppressing oxidative stress and promoting antioxidant system activity.

Anahtar Kelimeler

• Baicalin
• cypermethrine
• lipid peroxidation
• oxidative stress
• pyrethroid

Sipermetrine Maruz Kalan Sıçanlarda Baikalinin Lipid Peroksidasyon ve Oksidatif Hasar Üzerine Etkileri

Öz

Bu çalışmada sipermetrin (SPM) maruz kalan sıçanlarda baikalinin (BAİ) koruyucu etkilerinin araştırılması amaç- landı. Çalışmada her grupta 10 tane olacak şekilde toplam 40 adet 6-8 haftalık 180-220 g ağırlığında Wistar Albino ırkı erkek sıçan kullanıldı. Gruplar sırasıyla kontrol, BAİ (50 mg kg-1), SPM (25 mg kg-1) ve SPM (25 mg kg-1) + BAİ (50 mg kg-1) olarak belirlendi. 21 günlük deneme süresinin sonunda intraperitoneal ketamin-ksilazin anestezisi altında kalbe punksiyon yapılarak heparinize ve antikoagulansız özellikteki test tüplerine kan örneği alındı. Servikal dislokasyon son- rası sıçanlardan karaciğer, böbrek, beyin, testis, kalp ve akciğer dokuları çıkarıldı. Doku ve kan (plazma ve eritrosit) örneklerinde GSH, NO ve MDA düzeyleri ile GPx, GR, GST, SOD ve CAT enzim aktiviteleri mikroplaka okuyucuda spekrofotometrik yöntemle ölçülürken serumda LDH, AST, ALT ve ALP enzim aktiviteleri ile trigliserit, kolesterol, albü- min, total protein, BUN, ürik asit ve kreatinin düzeyleri Roche Cobas otoanalizöründe ölçüldü. Çalışma sonunda BAİ uygulanan grubun parametrelerinin kontrol grubuna benzer olduğu görüldü (P>0.05). Sipermetrin uygulanan grupta kontrole kıyasla doku GSH düzeyleri ile GPx, GR, GST, SOD ve CAT enzim aktivitelerinde anlamlı bir azalma tespit edildi (P<0.05). Malondialdehit ve NO seviyelerinde ise anlamlı bir artış kaydedildi (P<0.05). Ayrıca serum AST, ALT, ALP, LDH, kolesterol, trigliserit, BUN, ürik asit ve kreatinin düzey/aktivitelerinde artış görülürken serum total protein ve albümin düzeylerinde azalma görüldü (P<0.05). Sipermetrin ile BAİ’nin birlikte uygulandığı gruplarda SPM’nin etkileri kısmen ya da tamamen iyileştirdi. Sonuç olarak, BAİ'nin sıçanlarda oksidatif stresi baskılayarak ve antioksidan sistem aktivitesini artırarak SPM’nin neden olduğu oksidatif hasara karşı koruyucu etkiye sahip olabileceği ortaya konuldu.

Anahtar Kelimeler

• Baikalin
• lipid peroksidasyon
• oksidatif stres
• piretroid
• sipermetrin

Kaynakça

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