Bakteriyel Pnömonili Besi Sığırlarında Oluşan Serbest Radikal Hasarının Antioksidan Aktivite ve Bazı Mineral Maddeler Üzerine Etkisi

Abstract

Bu çalışmada, besi sığırlarında sık olarak görülen ve büyük ekonomik kayıplara neden olan bakteriyel enzootik pnömoni ile antioksidan

savunma arasındaki ilişkinin araştırılması amaçlanmıştır. Çalışma için klinik muayene bulgularına göre enzootik pnömoni teşhisi konan 50 baş

pnömonili (hasta grubu) ve 50 baş sağlıklı (kontrol grubu) olmak üzere toplam 100 besi sığırından usulüne uygun olarak v. jugularis’ten EDTA’lı

ve steril jelli cam tüplere kan örnekleri alınmıştır. Kan örneklerinde eritrositte glutatyon peroksidaz (GSH-Px), katalaz (CAT), süperoksit dismutaz

(SOD) enzim aktiviteleri ile glutatyon (GSH) düzeyleri; plazmada ise lipid peroksidasyon ürünü olan malondialdehit (MDA); serumda ise A, C, E

vitamini ve β-karoten ile bazı mineral düzeyleri çinko (Zn), bakır (Cu), selenyum (Se) tespit edilmiştir. Mikrobiyolojik muayeneler için burun svab

örnekleri alınarak genel ve spesifik besi yerlerine ekimleri yapıldıktan sonra uygun şartlarda inkube edilmiştir. Üreme görülen vasatlar enzootik

pnömoni etkenleri yönünden identifiye edilmiştir. Pnömonili hayvanlarda, lipid peroksidasyonun göstergesi olan MDA miktarında önemli bir şekilde

artış (P<0.001), CAT, GSH-Px, SOD enzim aktiviteleri ile vitamin (A, C, E ve β-karoten) ve Cu düzeylerinde önemli derecede (P<0.001) azalma

gözlenmiştir. GSH, Zn ve Se düzeylerinde gruplar arası farkın önemsiz olduğu (P>0.05) görülmüştür. Sonuç olarak, enzootik pnömonili sığırlarda

eritrosit antioksidan aktiviteleri (CAT, GSH-Px, SOD), vitamin (A, C, E ve β- karoten ) ile Cu düzeylerinin azalması ve MDA miktarlarındaki artışlar

hastalıkta oksidatif stresin geliştiğini göstermiştir.

Keywords

• Antioksidan,lipid peroksidasyonu,mineral madde,pnömoni,vitamin

The Effect of Free Radical Damage Formed in Beef Cattle With Bacterial Pneumoia on Antioxidant Activity and Some Minerals

Abstract

In this study, the relationship between antioksidant defence system and bacterial enzootic pneumonia which cause vast echonomic

loses wan aimed to investigate. For this study, blood samples were taken from 50 beef cattle having enzootic pneumonia diagnosed due to

clinical finding and blood samples from 50 healthy beef cattle determined clinically were also taken from jugular vein into steril tubes containing

EDTA. In whole blood; glutation peroxidase (GSH-Px ), catalase (CAT) and superoxide dismutase (SOD) activities were determined. In plasma;

malondialdehide (MDA) which is a product of lipid MDA, A, C, E vitamins and β- caroten and some mineral levels (Zn, Cu, Se) were determined.

For microbiological examinations nasal swab samples were taken and were plated into general and specific media and incubated under suitable

condition. The growth colonies were identified interms of enzootic pneumonia agents. In pneumonic animals lipid peroxidation indicator, MDA

increased significantly (P<0.001), but CAT, GSH-Px, SOD enzyme activities and vitamins (A, C, E and β- caroten) and Cu levels decreased

significantly (P<0.001). GSH, Zn, and Se levels were not different when companed between group (P<0.05). As a result, in enzootic pneumonic

cattle decrease in eritrocyte antioxidant activities (CAT, GSH-Px, SOD), vitamins (A, C, E and β- caroten) and Cu levels and increase in MDA

amount show the development of oxidative stres.

Keywords

• Antioxidants,lipid peroxidation,minerals,pneumonia,vitamins

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