Antioxidant Effects of Pomegranate Seed Oil Against Pentachlorophenol Toxicity in Rat Tissues

Year 2018, , 230 - 237, 04.12.2018

Zeynep Soyer Sarıca

https://doi.org/10.32707/ercivet.477307

Abstract

This study was
conducted to evaluate the effects of pomegranate seed oil PSO on the oxidative
stress induced by pentachlorophenol PCP in rats. Fourty Sprague-Dawley, 4-5
months old, 300g male rats were used in this study. Rats were assigned to four
groups each containing 10 animals. First group was control group; no
administration was made to animals in this group. Second, third and fourth
groups were given PSO at a dose of 0.15 ml/kg bw, PCP at a dose of 40 mg/kg bw
and PSO at a dose of 0.15 ml/kg bw+PCP at a dose of 40 mg/kg bw orally by
gavage for 28 days, respectively. At the end of the study, brain, liver,
kidney, testicle and spleen samples were collected. Malondialdehyde (MDA) level
and catalase (CAT), glutathione peroxidase (GSH-Px) and superoxide dismutase
(SOD) activities were measured in tissue samples. PCP caused an increase of MDA level in brain,
kidney, liver and testicle; a decrease of CAT activity in liver and spleen; a
decrease of GSH-Px activity in brain and testicle and a decrease of SOD
activity in brain, liver and kidney compared to control group. When PSO+PCP
group compared to PCP group, MDA level decreased in brain, liver and testicle;
CAT activity increased in spleen; SOD activity increased in brain and kidney.
When compared to the control group and the group given PSO+PCP, the data of
PSO+PCP group were generally similar to the control group. As a result,
administration of PCP at the dose of 40 mg/kg bw for 28 days caused oxidative
stress and administration of PSO at the dose of 0.15 ml/kg bw alleviated
severity of PCP induced oxidative stress

Keywords

Oxidative stress, pentachlorophenol, pomegranate seed oil, rat

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