Koç ve Boğa Abomazumunda Progesteron Reseptörünün İmmunlokalizasyonu

Year 2019, , 162 - 169, 01.12.2019

Narin Liman , Ergül Ergen Ural Kemal Kavraal Zelal Karakoç

https://doi.org/10.32707/ercivet.648519

Abstract

Progesteron (P4) memeli ovaryumunda korpus luteum tarafından
üretilen, hem dişi ve erkek üreme sistemlerinde hem de reprodüktif
olmayan doku ve organlarda çeşitli fonksiyonlar üstlenir. P4 hedef dokulardaki
etkilerini, progesteron reseptörü (PR) adı verilen spesifik hücre içi
reseptörler aracılığıyla gerçekleştirir. Çeşitli çalışmalar PR’lerinin hem
normal sağlıklı hem de kanserli mide dokularında eksprese edildiğini ve mide
mukozasının progesteronun eylemi için hedef doku olduğunu ortaya koymaktadır. PR
ekspresyonu insan, fare, rat ve mongol gerbil midesinde rapor edilmesine rağmen
koç ve boğa midelerinde bu reseptörün varlığına dair çalışma bulunmamaktadır.
Sunulan çalışmanın amacı PR’ünün erişkin koç ve boğa abomazumlarının kardiya,
fundus ve pilorus bölgelerinde eksprese edilip edilmediğini, PR ekspresyonunun
mide bölümlerininin yapısal özelliklerine bağlı bir değişim gösterip
göstermediğini immunohistokimyasal yöntemle belirlemek ve PR’ünün olası rolünü
değerlendirmektedir. Çalışmamızda her iki türün abomazum bölümlerinde yüzey
epitel hücreleri hariç, foveola gastrika'yı örten epitel hücrelerinde ve lamina
propriyadaki bez epitel hücrelerinde PR’ünün çekirdekten ziyade sitoplazmik ve
membransel yerleşim gösterdiği tespit edildi. Koç abomazumunun kardiya
bölümünde de bulunduğu gözlenen pariyetal hücreler ile her iki türün fundus
bölgesindeki pariyetal hücrelerde PR immunreaksiyonunun kuvvetli sitoplazmik
olduğu gözlendi. Pilorus bölgesinde foveola gastrika epitel hücrelerinin ve
müköz bez epitel hücrelerinin bazal sitoplazmalarının ve lateral membranlarının
PR için pozitif boyanma sergiledikleri görüldü. Bunların yanı sıra koç ve boğa
abomazumlarının her üç bölümünde tunika muskularisi oluşturan düz kas
hücrelerinde, lamina propriya ve tunika muskularisdeki kan damarlarının endotel
ve düz kas hücrelerinde sitoplazmik PR immunoreaksiyonu tespit edildi. Sonuç
olarak bu bulgular progesteronun, koç ve boğa midelerinde çeşitli biyolojik
süreçleri PR’leri aracılığıyla düzenlediğinin önemli bir kanıtı olabilir. 

Keywords

Abomazum, boğa, immunohistokimya, koç, progesteron reseptörü

The immunolocalization of Progestrone receptor in the abomasum of Ram and Bull

Abstract

Progesterone (P4), which is produced by the corpus
luteum in the mammalian ovary, undertakes various functions in both female and
male reproductive systems as well as non-reproductive tissues and organs. P4
effects the effects of target tissues via specific intracellular receptors
called progesterone receptor (PR). Several studies have demonstrated that PR is
expressed in both healthy and cancerous stomach tissues and that the gastric
mucosa is the target tissue for progesterone action. Although PR expression is
reported in human, mouse, rat and mongol gerbil stomach, there is no study of
the presence of this receptor in rams and bull abomasum. The purpose of the
present study was to investigate whether PR is expressed in adult ram and bull
abomasum, and whether the PR expression exhibits a change depending on the
structural properties of the abomasum regions, i.e. the cardia, fundus and
pylorus using immunohistochemistry, and to evaluate the possible function of PR. In both species, the epithelial cells lining
the foveola gastrica and the epithelial gland cells in the lamina propria,
except for surface epithelial cells, exhibited cytoplasmic and membranous
immunostaining for PR. The parietal cells of the fundus region in the abomasum
of both species and of the cardia region of ram abomasum displayed strongly
cytoplasmic PR immunoreactivity. The basal cytoplasm and lateral membranes of
the epithelial cells in the foveola gastrica and the epithelial cells of mucous
gland in the pylorus region showed positive staining for PR. In addition,
cytoplasmic PR immunoreactivity was detected in the smooth muscle cells of
tunica muscularis and in the endothelial and smooth muscle cells of blood
vessels in the abomasum wall. As a result, these findings may be an important
proof that P4 regulates biologically distinct processes in abomasum of ram and
bull through the action of the PRs.

Keywords

Abomasum, bull, immunohistochemistry, progesterone receptor, ram

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