Koç ve Boğa Abomazumunda Progesteron Reseptörünün İmmunlokalizasyonu

Öz

Progesteron (P4) memeli ovaryumunda korpus luteum tarafından üretilen, hem dişi ve erkek üreme sistemlerinde hem de reprodüktif olmayan doku ve organlarda çeşitli fonksiyonlar üstlenir. P4 hedef dokulardaki etkilerini, progesteron reseptörü (PR) adı verilen spesifik hücre içi reseptörler aracılığıyla gerçekleştirir. Çeşitli çalışmalar PR’lerinin hem normal sağlıklı hem de kanserli mide dokularında eksprese edildiğini ve mide mukozasının progesteronun eylemi için hedef doku olduğunu ortaya koymaktadır. PR ekspresyonu insan, fare, rat ve mongol gerbil midesinde rapor edilmesine rağmen koç ve boğa midelerinde bu reseptörün varlığına dair çalışma bulunmamaktadır. Sunulan çalışmanın amacı PR’ünün erişkin koç ve boğa abomazumlarının kardiya, fundus ve pilorus bölgelerinde eksprese edilip edilmediğini, PR ekspresyonunun mide bölümlerininin yapısal özelliklerine bağlı bir değişim gösterip göstermediğini immunohistokimyasal yöntemle belirlemek ve PR’ünün olası rolünü değerlendirmektedir. Çalışmamızda her iki türün abomazum bölümlerinde yüzey epitel hücreleri hariç, foveola gastrika'yı örten epitel hücrelerinde ve lamina propriyadaki bez epitel hücrelerinde PR’ünün çekirdekten ziyade sitoplazmik ve membransel yerleşim gösterdiği tespit edildi. Koç abomazumunun kardiya bölümünde de bulunduğu gözlenen pariyetal hücreler ile her iki türün fundus bölgesindeki pariyetal hücrelerde PR immunreaksiyonunun kuvvetli sitoplazmik olduğu gözlendi. Pilorus bölgesinde foveola gastrika epitel hücrelerinin ve müköz bez epitel hücrelerinin bazal sitoplazmalarının ve lateral membranlarının PR için pozitif boyanma sergiledikleri görüldü. Bunların yanı sıra koç ve boğa abomazumlarının her üç bölümünde tunika muskularisi oluşturan düz kas hücrelerinde, lamina propriya ve tunika muskularisdeki kan damarlarının endotel ve düz kas hücrelerinde sitoplazmik PR immunoreaksiyonu tespit edildi. Sonuç olarak bu bulgular progesteronun, koç ve boğa midelerinde çeşitli biyolojik süreçleri PR’leri aracılığıyla düzenlediğinin önemli bir kanıtı olabilir. 

Anahtar Kelimeler

• Abomazum,boğa,immunohistokimya,koç,progesteron reseptörü

The immunolocalization of Progestrone receptor in the abomasum of Ram and Bull

Öz

Progesterone (P4), which is produced by the corpus luteum in the mammalian ovary, undertakes various functions in both female and male reproductive systems as well as non-reproductive tissues and organs. P4 effects the effects of target tissues via specific intracellular receptors called progesterone receptor (PR). Several studies have demonstrated that PR is expressed in both healthy and cancerous stomach tissues and that the gastric mucosa is the target tissue for progesterone action. Although PR expression is reported in human, mouse, rat and mongol gerbil stomach, there is no study of the presence of this receptor in rams and bull abomasum. The purpose of the present study was to investigate whether PR is expressed in adult ram and bull abomasum, and whether the PR expression exhibits a change depending on the structural properties of the abomasum regions, i.e. the cardia, fundus and pylorus using immunohistochemistry, and to evaluate the possible function of PR. In both species, the epithelial cells lining the foveola gastrica and the epithelial gland cells in the lamina propria, except for surface epithelial cells, exhibited cytoplasmic and membranous immunostaining for PR. The parietal cells of the fundus region in the abomasum of both species and of the cardia region of ram abomasum displayed strongly cytoplasmic PR immunoreactivity. The basal cytoplasm and lateral membranes of the epithelial cells in the foveola gastrica and the epithelial cells of mucous gland in the pylorus region showed positive staining for PR. In addition, cytoplasmic PR immunoreactivity was detected in the smooth muscle cells of tunica muscularis and in the endothelial and smooth muscle cells of blood vessels in the abomasum wall. As a result, these findings may be an important proof that P4 regulates biologically distinct processes in abomasum of ram and bull through the action of the PRs.

Anahtar Kelimeler

• Abomasum,bull,immunohistochemistry,progesterone receptor,ram

Kaynakça

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