Deneysel Etilen Glikol Zehirlenmesi Oluşturulan Ratlarda Myokardiyal Etkilerin Araştırılması*,**

Abstract

Bu çalışmada etilen glikol (EG) toksikasyonunun ratlarda kalp kası üzerindeki muhtemel etkilerinin araştırılması amaçlandı. Sprague Dawley cinsi 30 adet erkek ve dişi rat çalışma materyalini oluşturdu. Tüm ratlar rastgele eşit sayı-da üç gruba ayrıldı. Birinci ve ikinci gruba gastrik gavaj ile EG (%99.5 saflıkta) verilmek suretiyle akut toksikasyon oluş-turuldu. Birinci gruba 3 ml/kg EG serum fizyolojikle (SF) sulandırılarak verilirken, ikinci gruba 6 ml/kg EG verildi. Üçün-cü gruba ise kontrol amacıyla yalnız SF verildi. Kan örnekleri 0 ve 72. ve 96. saattlerde kuyruk venasından alındı. Tok-sikasyonun klinik belirtileri görülen ratlardan EKG kayıtları alındı. İdrar sediment muayenesinde Ca-oksalat kristalleri tespit edildi. Ayrıca deneme sonunda nekropsi yapılarak ratların kalpleri histopatolojik analizler için alındı. Ortalama kan üre (BUN), laktat dehidrogenaz (LDH), glukoz ve kreatinin düzeylerinin toksikasyon oluşturulan ratlarda önemli oranda arttığı, kalsiyum düzeylerinin ise azaldıgı görüldü. Ortalama kardiyak troponin T (cTn-T) ve kreatin-kinaz myo-kardial band (CK-MB) seviyelerinin toksikasyon oluşturulan gruplarda 0. saatler ve kontrol grubuna göre yüksek olduğu görüldü. Ancak cTn-I seviyelerinde ise bir farklılık belirlenmedi. İdrar analizlerinde Ca-oksalat kristalleri belirlendi. Nek-ropsi bulgularında; epikardiyal kanama ve histopatolojide; nekroz alanlarını çevreleyen mononükleer hücreler, suben-dokard bölgesinde hücresel infiltrasyon odakları, dejenerasyon ve interstisyel ödem saptandı. Bu çalışmada verilen doza bağlı olarak elektrokardiyografi (EKG), biyokimya ve histopatoloji bulgularında önemli değişikliklerin görülmesi nedeniyle, EG zehirlenmesinde toksik miyokardial hasarının oluştuğu sonucuna varılmıştır.

Keywords

• Ca-oksalat
• etilen glikol
• miyokard
• rat
• toksikasyon

Investigation of Myocardial Effects in Rats Subjected to Experimental Ethylene Glycol Toxicity

Abstract

The aim of this study was to investigate possible effects of experimental ethylene glycol (EG) toxication on the heart muscle in rats. Thirty male and female rats of Sprague Dawley formed the study material. All rats were ran-domly divided into three groups and gastric gavage method were used for the experimental toxication with EG (99.5% purity). 3 ml/kg EG diluted with saline (Serum Physiologic, SF) was given to the first group and 6 ml/kg EG diluted with SF to the second group, only SF same volume with EG was given to third group as control. Blood samples were taken from the tail vein at the 0 (before EG application) and 72th hour and at the 96th hour after EG application. ECG meas-urements were performed to the rats showing clinical symptoms of the toxication. ECG recordings were obtained from rats with clinical signs of toxicity. Urine samples were taken and their sediments were analyzed. In addition, necropsy was performed at post experiment and the hearts of the rats were taken for cardiac histopathology. In biochemical analyzes, it was observed that blood ürea (BUN), lactate dehydrogenase (LDH), glucose and creatinine levels in-creased and calcium levels decreased significantly. Mean cardiac troponin T (cTnT) and creatine-kinase myocardial band (CK-MB) levels were found to be different in groups that formed toxicity compared to control group. However, there was no difference in cTn-I levels. In addition, Ca-oxalate crystals were detected in urine analysis. In necropsy, epicardial bleeding and in histopathology mononuclear cells surrounding the necrosis areas, cellular infiltration foci in the subendocardium, degeneration and interstitial edema were detected. In the light of the findings obtained in this study, electrocardiography (ECG) and histopathological findings showed that a toxic myocardial injury also occurred in EG poisoning.

Keywords

• Ca-oxalate
• ethylene glycol
• myocardium
• rat
• toxication

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