Microbiological Quality Evaluation of Various Types Of Cheese

Year 2018, Volume 15, Issue 2, 86 - 93, 15.08.2018

Pelin KOÇAK KIZANLIK

Abstract

This study was conducted to determine microbiological quality of various cheese samples. A total of 120 cheese samples (30 white brined cheese, 30 tulum cheese, 30 kashar cheese, 30 lor cheese) were collected from dairy processing plants' output shops. Cheese samples produced in various processing plants located in Western Ae-gean region of Turkey (İzmir, Aydın, Muğla) were obtained from the output shops in Aydın. Enumeration of total viable count (TVC), Staphylococcus aureus and coliforms were carried out, and the presence of Escherichia coli, Salmonella spp. and Listeria monocytogenes were investigated. The results showed that the mean TVC in white brined cheese, Tulum cheese, kashar cheese and lor cheese were 9.43 log cfu/g, 9.87 log cfu/g, 7.71 log cfu/g and 9.80 log cfu/g, respectively. The mean numbers of S. aureus were found in these samples 4.87 log cfu/g, 5.61 log cfu/g, 4.14 log cfu/g and 5.09 log kob/g, respectively. When the levels of coliform bacteria considered, the least contaminated cheese was found to be kashar. The presences of E. coli and L. monocytogenes were determined in 32 samples (26.6%) and one kashar cheese sample (0.83%), respectively. No Salmonella spp. were encountered in any of the cheese samples investigated. It was concluded that microbiological quality of the cheese samples were generally poor due to inade-quate hygienic conditions during production processes and marketing. High numbers of S. aureus contaminated cheese samples may cause food poisoning cases due to its enterotoxins. Contamination with L. monocytogenes and L. ivanovii may also cause public health risks.

Keywords

Halk sağlığı, mikrobiyolojik kalite, peynir

Abstract

References

• 1. Akineden O, Hassan AA, Schneider E, Usleber E. Enterotoxigenic properties of Staphylococcus aureus isolated from goats’ milk cheese. Int J Food Microbiol 2008; 124(2): 211-6. 2. Akkaya L, Alişarlı M. Afyonkarahisar’da tüketime sunulan peynirlerde Listeria monocytogenes veSalmonella spp. varlığının belirlenmesi. YYU Vet Fak Derg2006; 17(1-2): 87-91. 3. Almeida G, Figueiredo A, Rôla M, Barros RM, Gibbs P, Hogg T, Teixeira P. Microbiological characterization of randomly selected Portuguese raw milk cheese with reference to food safety. J Food Protect 2007; 70(7): 1710-6. 4. Arslan S, Ozdemir F. Prevalence and antimicrobial resistance of Listeria spp. in homemade white cheese. Food Control 2008; 19(4): 360-3. 5. Aygun O, Aslantas O, Oner S. A survey on the microbiological quality of Carra, traditional Turkish cheese. J Food Eng 2005;66(3):401-4. 6. Aygun O, Pehlivanlar S. Listeria spp. in raw milk and dairy products in Antakya, Turkey. Food Control 2005; 17(8): 676-9. 7. Brooks JC, Martinez B, Stratton A, Bianchini A, Krokstrom R, Hutkins R. Survey of raw milk cheeses for microbiological quality and prevalence foodborne pathogens. Food Microbiol2012; 31(2): 154-8. 8. Büyükyörük S, Göksoy EÖ. Aydın ilinde satışa sunulan köy peynirlerinde Listeria varlığının araştırılması. Uludag Univ Vet Fak Derg 2011; 30(1): 9-12. 9. Callon C, Gilbert FB, Cremoux RD, Montel MC. Application of variable number of tandem repeat analysis to determine the origin of S. aureus contamination from milk to cheese in goat cheese farms. Food Control 2008; 19(2): 143-50. 10. Cokal Y, Dagdelen A, Cenet O, Gunsen U. Presence ofL. monocytogenes and some bacterial pathogens in two Turkish traditional foods, Mihalic cheese and Hosmerim desert. Food Control 2012;26(2): 337-40. 11.Colak H, Hampikyan H, Bingöl EB, Ulusoy B. Prevalance of L. monocytogenes and Salmonella spp. in tulum cheese. Food Control 2007; 18(5): 576-9. 12. D’Aoust J, Maurer J, Bailey JS. Salmonella species. DoyleMP, BeuchatLR, MontvilleTJ. eds. In: Food Microbiology: Fundamentals and Frontiers. Washington: ASM Press, 2001; pp. 141-78. 13.De Buyser ML, Dufour B, Maire M, Lafarge V. Implication of milk and milk products in food-borne diseases in France and in different industrialised countries. Int J Food Microbiol 2001; 67(1-2): 1-17. 14. De la Rua-Domenech R. Human Mycobacterium bovis infection in the United Kingdom: Incidence, risks, control measures and rewiev of the zoonotic aspects of bovine tuberculosis. Tuberculosis 2006; 86(2): 77-109. 15. Erkan EM, Ciftcioglu G, Vural A, Aksu H. Some microbiological characteristics of herbed cheeses. J Food Quality 2007; 30(2): 228-36. 16. Fox PF, McSweeny PLH. Cheese: An overview. Fox PF, McSweeny PLH, Cogan TM, Guinee TP. eds. In: Cheese: Chemistry, Physics and Microbiology.Third Edition.California: Elseiver Academic Press, 2004; pp. 1-5. 17. Gould LH, Mungai E, Behrayesh CB. Outbreaks attributed to cheese: Differences between outbreaks caused by unpasteurized and pasteurized dairy products, United States, 1998-2011. Foodborne Pathog Dis 2014; 11(7): 545-51. 18. Gould LH, Walsh KA, Vieira AR, Herman K, Williams IT, Hall AJ, Cole D. Surveillance for foodborne disease outbreaks-United States, 1998-2008. Morbiditiy and Mortality Weekly Report, Surveillance Summaries 2013; 62(2): 1-34. 19. Gülmez M, Güven A. Beyaz ve çeçil peynirlerinde Campylobacter, Salmonellave Listeriatürlerinin araştırılması. Kafkas Univ Vet Fak Derg 2001; 72(2): 155-61. 20. Günşen U, Büyükyörük İ. Piyasadan temin edilen taze kaşar peynirlerinin bakteriyolojik kaliteleri ile aflatoksin M1 düzeylerinin belirlenmesi. Turk J Vet Anim Sci 2003; 27(4): 821-5. 21. Halkman K, 2005. Gıda Mikrobiyolojisi Uygulamaları. Birinci Baskı. Ankara: Başak Matbaacılık, 2005; pp:158-9. 22. Hayaloglu AA, Kirbag S. Microbial quality and presence of moulds in Kuflu cheese. Int J Food Microbiol 2007; 115(3): 376-80. 23. Jablonski LM, Bohach G. Staphylococcus aureus. DoyleMP, BeuchatLR, MontvilleTJ. eds. In: Food Microbiology Fundamentals and Frontiers. Second Edition. Washington: ASM Press, 1997; pp. 353-7. 24. Jorgensen HJ, Mork T, Hogasen HR, Rorvik LM. Enterotoxigenic Staphylococcus aureus in bulk milk Norway. J Appl Microbiol 2005; 99(1):158-66. 25. Kahraman T, Gürsel O, Ozinan B, Goksoy EO. Prevalance of Salmonella spp. and Listeria monocytogenes in different cheese types produced in Turkey. Brit Food J 2010; 112(11): 1230-6. 26. Kaynar Z, Kaynar P, Kocak C. Ankara piyasasında tüketime sunulan beyaz peynirlerin hijyenik kalitelerinin belirlenmesi üzerine bir çalışma. Türk Hij Den Biyol Derg 2005; 62(1):1-10. 27. Keskin Y, Özyaral O, Başkaya R, Susur MA. Semt pazarlarında satılan beyaz peynirlerin mikrobiyolojik kalitesinin araştırılması. Türk Mikrobiyol Cem Derg 2006; 36(1):9-19. 28. Kousta M, Mataragas M, Skandamis P, Drosinos EH. Prevalence and sources of cheese contamination with pathogens at farm and processing levels. Food Control 2010; 21(6): 805-15. 29. Kosikowski FV, Mistry VV. Fundamentals of cheese making and ripening. Kosikowski FV. ed.In: Cheese and Fermented Milk Foods. Third Edition. Westport: Limited Liabilty Company, 1997; pp. 109-12. 30. Kursun O, Kırdar SS, Kale ASA, Guner A. Burdur’da tüketime sunulan beyaz salamura peynirlerin mikrobiyolojik kalitesinin belirlenmesi. Onuncu Ulusal Gıda Kongresi. Mayıs, 21-23, 2008; Erzurum-Türkiye. 31. Le Loir Y, Baron F, Gautier M. Staphylococcus aureus and food poisoning. Genet Mol Res 2003; 2(1): 63-76. 32. Little CL, Rhoadesa JR, Sagooa SK, Harrisa J, Greenwoodb M, Mithania V, Granta K, McLauchlina J. Microbiological quality of retail cheeses made from raw, thermized or pasteurized milk in the UK. Food Microbiol 2008; 25(2): 304-12. 33. Miranda JM, Mondragón AC, Martinez B, Guarddon M, Rodriguez JA. Prevalence and antimicrobial resistance of Salmonella from different raw foods in Mexico. J Food Protect 2009; 72(5): 966-71. 34. Öksüztepe G, Patır B, Dikici A, İlhak I. Elazığ’da tüketime sunulan vakum paketli taze kaşar peynirlerinin mikrobiyolojik ve kimyasal kalitesi. F Ü Sağ Bil Vet Derg 2009; 23(2): 89-94. 35. Roberson JR, Fox LK, Hancock DD, Gay JM, Besser TE. Ecology of Staphylococcus aureus isolated from various sites on dairy farms. J Dairy Sci 1994; 77(11): 3354-64. 36. Rudolf M, Scherer S. High incidence of Listeria monocytogenes in European red smear cheese. Int J Food Microbiol 2001; 63(11): 91-8. 37. Sagun E, Sancak Y, Işleyici O, Ekici K. Van ve çevresi süt ve otlu peynirlerinde Listeria türlerinin varlığı ve yaygınlığı üzerine bir araştırma. Turk J Vet Anim Sci 2001; 25: 15-9. 38. Simsek B, Sagdic O. Isparta ve yöresinde üretilen dolaz (tort) peynirinin bazı kimyasal ve mikrobiyolojik özellikleri. SüleymanDemirel Üni Fen Bil Ens Derg 2006; 10(3): 346-51. 39. Takahashi I, Johns CK. S. aureus in cheddar cheese. J Dairy Sci 2010; 42(6): 1032-7. 40. Tekinsen KK, Ozdemir Z. Prevalence of foodborne pathogens in Turkish Van otlu (Herb) cheese. Food Control 2006; 17(9): 707-11. 41. Temelli S, Anar Ş, Şen C, Akyuva P. Determination of microbiological contamination sources during Turkish white cheese production. Food Control 2006; 17(11): 856-61. 42. Temiz A. Gıdalarda mikrobiyolojik gelişmeyi etkileyen faktörler. Ünlütürk A, Turantaş F. eds. In: Gıda Mikrobiyolojisi. Üçüncü Baskı. İzmir: Meta Basım Matbaacılık Hizmetleri, 2003; ss. 85-106. 43. Torkar GK, Teger GS. The presence of some pathogen microorganisms, yeast and moulds in cheese samples produced at small dairy-processing plants. Acta Agric Slov 2006; 88(1): 37-51. 44. Türk Gıda Kodeksi. Türk Gıda Kodeksi Mikrobiyolojik Kriterler Tebliği. Resmi Gazete 29.12.2011. Türkiye Cumhuriyeti Gıda Tarım ve Hayvancılık Bakanlığı,Ankara: 2011; pp. 1-31. 45. Türk Standartları Enstitüsü. Gıda ve hayvan yemlerinin-mikrobiyolojisi-koagulaz-pozitif Stafilokokların (Staphyloccus aureus ve diğer türler) sayımı için yatay metot-bölüm 1: baird-parker agar besiyeri kullanarak. TSE 6582-1 EN ISO 6888-1A,Türk Standartları Enstitüsü, Ankara: 2001; ss.1-9.4. 46. Türk Standartları Enstitüsü. Süt ve süt ürünleri-Salmonella spp. aranması. TSE 8907 ISO 6785,Türk Standartları Enstitüsü, Ankara: 2003; ss. 1-15. 47. Türk Standartları Enstitüsü. Gıda ve yem maddelerinin mikrobiyolojisi-Listeria monocytogenes’in aranması ve sayımı metodu bölüm 1: arama metod. TSE EN ISO 11290-1/A1,Türk Standartları Enstitüsü: Ankara: 2004; ss.1-9. 48. Türk Standartları Enstitüsü. Gıda ve hayvan yemleri mikrobiyolojisi-Koliformların tespiti ve sayımı için yatay yöntem-En muhtemel sayı tekniği. TS ISO 4831, Türk Standartları Enstitüsü, Ankara: 2010; ss.1-7. 49. Türk Standartları Enstitüsü. Gıda zinciri mikrobiyolojisi-Mikroorganizmaların sayımı için yatay yöntem-Bölüm 2: Yüzey kaplama tekniğiyle 30°C’ta koloni sayımı. TS EN ISO 4833-2, Türk Standartları Enstitüsü, Ankara: 2014; ss.1-23. 50. Vautor E, Abadie G, Guibert JM, Huard C, Pepin M. Genotyping of Staphylococcus aureus isolated from various sites on farms with dairy sheep using pulsed-field gel electrophoresis. Vet Microbiol 2003; 96(1): 69-79.