The Immunoreactivity of Vimentin in Angora Rabbit Palatine Tonsil

Year 2009, Volume: 6 Issue: 2, 105 - 110, 01.06.2009

F. Beyaz

Abstract

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Keywords

M hücresi, tavşan, tonsilla, vimentin

Ankara Tavşanı Tonsilla Palatinasında Vimentin İmmunreaktivitesi

Abstract

Çalışma, Ankara tavşanı tonsilla palatinasında vimentin immunreaktivitesini ortaya koymak amacıyla planlandı.Araştırmada, 10 adet sağlıklı erişkin Ankara tavşanından alınan tonsilla palatinalar kullanıldı. İmmunohistokimyasalboyamalar için Strept-ABC boyama metodu uygulandı. Monokriptik olan tonsilla palatina, içerisinde çok sayıda lenfoidhücre bulunan ve lenf foliküllerinin kriptle temasını sağlayan retiküler epitel ile bunların arasında kalan alanlarda çokkatlı yassı keratinize olmayan epitelle çevrelenmişti. Retiküler epitelde, vimentin pozitif immunreaksiyon gözlenirken,çok katlı yassı keratinize olmayan epitelde immunreaktivite saptanmadı. Retiküler epiteldeki vimentin pozitif hücreler 35 adet intraepiteliyal lenfositi bazolateral sitoplazmalarıyla çevrelemeleriyle karakterizeydi. Retiküler epitel içerisinde vesubepiteliyal alanlarda morfolojik olarak makrofajlara benzeyen bir kaç iri hücrenin sitoplazmasında ve sitoplazmikuzantılara sahip dendritik hücre benzeri hücrelerde kuvvetli vimentin immunreaktivitesi belirlendi. Ayrıca, folikül içerisindeki kan kapilarları ve interfoliküler alanlarda yerleşim gösteren yüksek endotelli venüllerin endotelinde de kuvvetlivimentin pozitif immunboyanma gözlendi. Sunulan çalışmayla, Ankara tavşanı tonsilla palatinasında vimentinimmunreaktivitesinin M hücreleri, makrofajlar, dendritik hücreler ve damar endotellerinde olmak üzere çok geniş birdağılım sergilediği ortaya konmuştur

Keywords

M hücresi, tavşan, tonsilla, vimentin

References

• Belz GT, Heath TJ, 1995. The epithelium of canine palatine tonsils. Anat Embryol, 192: 194.
• Belz GT, Heath TJ, 1996. Tonsils of the soft palate of young pigs: Crypt structure and lymphoepithelium. Anat Rec, 245: 102-113.
• Beyaz F, 2004. M hücreleri: Membranöz epitel hücreleri. Erciyes Üniv Vet Fak Derg, 1(2): 138.
• Carapelli A, Regoli M, Nicoletti C, Ermini L, Fonzi L, Bertelli E, 2004. Rabbit tonsil- associated M-cells express cytokeratin 20 and take up particulate antigen. J Histochem Cytochem, 52: 1323-1331.
• Cesta MF, 2006. Normal structure, function, and histology of mucosa-associated lymphoid tissue. Toxicol Pathol, 34: 599-608.
• Cocquyt G, Baten T, Simoens P, Van Den Broeck W, 2005. Anatomical localisation and histology of the ovine tonsils. Vet Immunol Immunopathol, 107: 79-86.
• Gebert A, Hach G, 1992. Vimentin antibodies stain membranous epithelial cells in the rabbit bronchus-associated Histochemistry, 98: 271-273. lymphoid (BALT).
• Gebert A, Hach G, Bartels H, 1992. Co- localization of vimentin and cytokeratins in
• M-cells of rabbit gut-associated lymphoid tissue (GALT). Cell Tissue Res, 269: 331-340. Gebert A, 1995. Identification of M-cells in the rabbit tonsil by vimentin immunohistochemistry and in vivo protein transport. Histochem Cell Biol, 104: 211-220.
• Gebert A, Willführ B, Pabst R, 1995. The rabbit M-cell marker vimentin is present in epithelial cells of the tonsil crypt. Otolaryngol, 115: 697-700. Acta
• Gebert A, 1996. M-cells in the rabbit tonsils exhibit distinctive glycoconjugates in their apical membranes. J Histochem Cytochem, : 1033-1042.
• Belz GT, Heath TJ, 1996. Tonsils of the soft palate of young pigs: Crypt structure and lymphoepithelium. Anat Rec, 245: 102-113.
• Beyaz F, 2004. M hücreleri: Membranöz epitel hücreleri. Erciyes Üniv Vet Fak Derg, 1(2): 138.
• Carapelli A, Regoli M, Nicoletti C, Ermini L, Fonzi L, Bertelli E, 2004. Rabbit tonsil- associated M-cells express cytokeratin 20 and take up particulate antigen. J Histochem Cytochem, 52: 1323-1331.
• Cesta MF, 2006. Normal structure, function, and histology of mucosa-associated lymphoid tissue. Toxicol Pathol, 34: 599-608.
• Cocquyt G, Baten T, Simoens P, Van Den Broeck W, 2005. Anatomical localisation and histology of the ovine tonsils. Vet Immunol Immunopathol, 107: 79-86.
• Gebert A, Hach G, 1992. Vimentin antibodies stain membranous epithelial cells in the rabbit bronchus-associated Histochemistry, 98: 271-273. lymphoid (BALT).
• Gebert A, Hach G, Bartels H, 1992. Co- localization of vimentin and cytokeratins in
• M-cells of rabbit gut-associated lymphoid tissue (GALT). Cell Tissue Res, 269: 331-340. Gebert A, 1995. Identification of M-cells in the rabbit tonsil by vimentin immunohistochemistry and in vivo protein transport. Histochem Cell Biol, 104: 211-220.
• Gebert A, Willführ B, Pabst R, 1995. The rabbit M-cell marker vimentin is present in epithelial cells of the tonsil crypt. Otolaryngol, 115: 697-700. Acta
• Gebert A, 1996. M-cells in the rabbit tonsils exhibit distinctive glycoconjugates in their apical membranes. J Histochem Cytochem, : 1033-1042.
• Gebert A, 1997. M cells in the rabbit palatine tonsils: the distribution, spatial arrangement and membrane subdomains as defined by confocal lectin histochemistry. Anat Embryol, : 353-358. Girard J-P, Springer TA, High endothelium venules (HEVs): specialization endothelium Immunol Today, 16(9): 449-457. migration.
• Haley PJ, 2003. Species differences in the structure and function of the immune system. Toxicology, 188: 49-71.
• Hermann H, Bar H, Kreplak L, Strelkov SV, Aebi U, 2007. Intermediate filaments: from cell architecture to nanomechanics. Nature Rev, 8: 573.
• Horter DC, Yoon KJ, Zimmerman JJ, 2003. A review of porcine tonsils in immunity and disease. Anim Health Res Rev, 4(2): 143-155.
• Howie AJ, 1980. Scanning and transmission electron microscopy on the epithelium of human palatine tonsils. J Pathol, 130: 91-98.
• Howie AJ, 1982. The cells in tonsillar crypts.
• Clin Otolaryngol Allied Sci, 7(1): 35-44. Ivaska J, Pallari H-M, Nevo J, Eriksson JE, Novel functions of vimentin in cell adhesion, migration, and signaling. Exp Cell Res, 313: 2050-2062.
• Jepson MA, Mason CM, Bennett MK, Simmons NL, Hirst BH, 1992. Co-expression of vimentin and cytokeratins in M cells of rabbit intestinal epithelium. Histochem J, 24: 33-39. follicle-associated
• Koshi R, Yardulak M, Perry ME, 2001.
• Vimentin, cytokeratin 8 and cytokeratin 18 are not spesific markers for M-cells in human palatine tonsils. J Anat, 199: 663-674. Kumar P, Timoney JF, 2005. Histology, immunohistochemistry and ultrastructure of the equine palatine tonsil. Anat Histol Embryol, : 192-198.
• Liebler-Tenorio EM, Reinhard P, 2006. MALT structure and function in farm animals. Vet Res, 37: 257-280.
• Nave H, Gebert A, Pabst A, 2001. Morphology and immunology of the human palatine tonsil. Anat Embryol, 204: 367-373.
• Olah I, Everett NB, 1975. Surface epithelium of the rabbit palatine tonsil: scanning and transmission electron microscopic study. J
• Reticuloendothel Soc, 18: 53-62. Olah I, Takacs L, Törö I, 1988. Formation of lymphoepithelial tissue in the sheep’s palatine tonsil. Acta Otolaryngol, 105: 7-17.
• Pabst R, 2007. Plasticity and heterogeneity of lymphoid organs What are criteria to call a lymphoid organ primary, secondary or tertiary? Immunol Letters, 112: 1-8.
• Perry M, 1994. The specialised structure of crypt epithelium in the human palatine tonsil and its fuctional significance. J Anat, 185: 111
• Perry M, Whyte A, 1998. Immunology of the tonsils. Rev Immunol Today, 19(9): 414-421.
• Surjan LJ, 1987. Tonsils and lympho-epithelial structures in the pharynx as immuno-barriers. Acta Otolaryngol, 103: 369-372. Surjan LJ, Immunohistochemical markers of tonsillar crypt epithelium. Acta Otolaryngol, 454: 60-63.
• Suzumoto M, Hotomi M, Fujihara K, Tamura S, Kuki K, Tohya K, Kimura M, Yamanaka N, Functions of tonsils in the mucosal immune system of the upper respiratory tract using a novel animal model, Suncus murinus. Acta Otolaryngol, 126: 1164-1170. Van Kempen MJP, PB, The Cauwenberge response in adenoids and tonsils. Int Arch Allergy Immunol, 122: 8-19. immune
• Zidan M, Pabst R, 2009. The microanatomy of the palatine tonsils of the one-humped camel
• (Camelus dromedarius). Anat Rec, 292: 1192