Kimya Öğrencilerinde, Kimyasalların Genotoksik Etkisinin Kromozom Aberasyon Testiyle Belirlenmesi

Year 2011, Volume: 27 Issue: 2, 217 - 221, 01.04.2011

Bilge Erol Songül Budak Diler

Abstract

Üniversitelerin kimya bölümünde okuyan öğrenciler, laboratuar çalışmaları esnasında çeşitli kimyasallara maruz kalırlar. Bu kimyasalların öğrencilerde genotoksik risk oluşturup oluşturmadığı, in vitro lenfosit kültüründe, kromozom aberasyonu (KA) testiyle belirlendi. Bu çalışmada, Niğde Üniversitesi Fen Edebiyat Fakültesi Kimya Bölümü öğrencileri ile kimyasala maruz kalmayan öğrencilerden periferal kan örnekleri alındı. Deney ve kontrol grubundan elde edilen preparatların incelenmesi ile kromozom anormallikleri ve mitotik indeks belirlendi. Bu iki gruptan elde edilen veriler, t-testi ile karşılaştırıldı ve laboratuar çalışmalarının öğrencilerde kromozom anormalliklerini kontrole göre arttırmadığı ve mitotik indeksi de önemli derecede değiştirmediği saptandı (p>0.05).

Keywords

Kimyasal madde, genotoksik etki, lenfosit kültürü, kromozomaberasyonu

Determination of chemically induced genotoxicity by chromosomal aberration test in chemistry students

Abstract

University students studying chemistry at degree level are exposed to various chemicals during their laboratory works. To determine if this exposure constitutes a genotoxic risk factor, lymphocytes from exposed subjects were examined in vitro by employing the chromosomal aberration (CA) test. Peripheral blood samples were taken from the subjects who were students at the Chemistry Division of the Faculty of Literature and Sciences, University of Nigde. Controls consisted of unexposed students. Chromosomal anomalies and mitotic index of peripheral blood cells from all subjects were determined. The results from the two groups were compared by the t-test. There were no significant differences between the two groups in terms of CA and mitotic index (p>0.05).

Keywords

Chemical substance, genotoxic effect, lymphocyte culture, chromosomal aberration

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