GÜNEYDOĞU ANADOLU BÖLGESİ İÇİN ENDEMİK OLAN TATLISU SALYANGOZU Pseudamnicola bilgini (SCHÜTT, 1992) (GASTROPODA: PROSOBRANCHIA)’NİN YAĞ ASİTİ KOMPOZİSYONU

Yıl 2009, Cilt: 25 Sayı: 1, 48 - 58, 01.02.2009

İhsan Ekin Mehmet Başhan Rıdvan Şeşen

Öz

Bu çalışmada, Ekim 2007 tarihinde, Mardin Sultanköy kaynak sularından toplanan endemik tatlısu salyangozu Pseudamnicola bilgini'nin total vücut lipitleri, ince tabaka kromatografi ile fraksiyonlandı. Salyangozun, total vücut lipitleri ile fosfolipit ve nötral lipit fraksiyonundaki yağ asitleri gaz kromatografi ve gaz kromatografi-kütle spektrometresi ile analizlendi. Analizlerde, doymuş yağ asitlerinden C12:0, C13:0, C14:0, C15:0, C16:0, C17:0, C18:0; tekli doymamış yağ asitlerinden C16:1ω7, C18:1ω9, C20:1ω9 ve çoklu doymamış yağ asitlerinden C18:2ω6, C18:3ω3, C20:2ω6, C20:3ω6, C20:4ω6, C20:5ω3 ve C22:6ω3 asitler tespit edildi. Tek karbonlu ve 20 karbonlu çoklu doymamış yağ asitlerinin yapıları, gaz kromatografi-kütle spektrometre ile doğrulandı. Analizlerde elde edilen yağ asitlerinin yüzde değerleri birbirleri ile karşılaştırıldı. Yüzde dağılımda en çok C18:1ω9 (%16.2- %33.0), C16:0 (%21.3-%24.0) ve C18:2ω6 (%8.5-%14.5) asitler tespit edildi.

Anahtar Kelimeler

Yağ asitleri, Pseudamnicola bilgini, Endemik tatlısu salyangozu, Güneydoğu Anadolu

FATTY ACID COMPOSITION OF FRESHWATER SNAIL Pseudamnicola bilgini (SCHÜTT, 1992) (GASTROPODA: PROSOBRANCHIA), ENDEMIC IN SOUTHEAST ANATOLIA, TURKEY

Öz

In this study, body lipids of an endemic freshwater snail Pseudamnicola bilgini collected from Sultanköy in Mardin in October 2007 were fractionated by thin layer chromatography. Total lipids, fractionated lipids such as phospholipids and neutral lipids of whole snail were analyzed by capillary gas chromatography and gas chromatography-mass spectrometry. In the analysis, C12:0, C13:0, C14:0, C15:0, C16:0, C17:0, C18:0 saturated fatty acids; C16:1ω7, C18:1ω9, C20:1ω9 monounsaturated fatty acids and C18:2ω6, C18:3ω3, C20:2ω6, C20:3ω6, C20:4ω6, C20:5ω3, C22:6ω3 polyunsaturated fatty acids were found. The structures of odd-numbered fatty acids and C20 polyunsaturated fatty acids were confirmed by gas chromatography-mass spectrometry. The percentages of fatty acids in the lipid fractions were compared with each other. In the analyses, C18:1ω9 (16.2%-33.0%), C16:0 (21.3%-24.0%) and C18:2ω6 (8.5%-14.5%) acids were the most abundant components.

Anahtar Kelimeler

Fatty acids, Pseudamnicola bilgini, Endemic freshwater snail, the Southeast Anatolia

Kaynakça

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