Topraktan İzole Edilen Bacillus Türlerinin Biyokalsifikasyon Özelliklerinin Belirlenmesi

Year 2022, Volume: 38 Issue: 1, 147 - 154, 30.04.2022

Fatma Öztürk Küp , Fatma Eroğlu

Abstract

Bu çalışma, üreaz aktivitesine sahip Bacillus türlerinin topraktan izolasyonunu, morfolojik ve moleküler tanımlamalarını ve biyokalsifikasyon özelliklerini belirlemeyi amaçlamıştır. Toprak örneklerinden Bacillus türüne ait toplam 11 bakteri izole edilmiştir. İzolatların tanımlanması, 16S rRNA dizileme analizi ile gerçekleştirilmiştir. İzolatların üreaz aktiviteleri fenol-hipoklorit yöntemine göre gerçekleştirilmiştir. Sıvı ortamda CaCO3 birikimi belirlenmiştir. CaCO3 kristalleri, taramalı elektron mikroskobunda (SEM) analiz edilmiştir. İzolatların üreaz enzim aktiviteleri kontrol grubu Sporosarcina pasteurii ile karşılaştırılmıştır. Sonuçlara göre F10 (Bacillus mycoides) spesifik üreaz aktivitesi 8.50±0.00 U/mg ile en yüksek aktiviteye sahip tür olarak belirlendi. En düşük spesifik aktiviteye sahip bakteri, 2,75±0,04 U/mg spesifik üreaz aktivitesi ile F14 (Bacillus thuringiensis) olarak belirlendi. Üre ve kalsiyum klorür katkılı sıvı ortamda S. pasteurii'de maksimum CaCO3 birikimi 0,53 g olarak belirlendi. Kontrol suşundan sonra maksimum CaCO3 birikimi F9, F13 ve F8 izolatlarında sırasıyla 0.20g, 0.20g ve 0.18g olarak ölçülmüştür. SEM incelemelerinin sonucuna göre izolatların kalsit birikimini gerçekleştirdiği belirlenmiştir.

Keywords

Biyokalsifikasyon, Bacillus, 16S rRNA, Ureaz aktivitesi

Project Number

FLY 2014-5560

Determination of Biocalcification Properties of Bacillus Species Isolated from Soil

Abstract

This study aimed to determine isolation of Bacillus species having urease activityfrom soil, morphological and molecular identification and on biocalcification features. Total 11 bacteria belong to Bacillus species were isolated from soilsamples. Identification of the isolates was carried out with 16S rRNA sequencing analysis. Urease activity of the isolates was carried out according to the phenol-hypochlorite method. CaCO3 accumulation was determined and liquid media. CaCO3 crystals were analyzed in the scanning electron microscope (SEM). Urease enzyme activities of the isolates were compared to with control group Sporosarcina pasteurii. According to results, F10 (Bacillus mycoides) was determined as species having the highest activity with specific urease activity 8.50±0.00 U/mg. The bacterium having the lowest specific activity was determined F14 (Bacillus thuringiensis) with specific urease activity 2.75±0.04 U/mg value. In liquid medium supplemented with urea and calcium chloride, the maximum CaCO3 accumulation was determined to be 0.53 g in S. pasteurii. After the control strain, the maximum CaCO3 accumulation was measured as 0.20g, 0.20 g and 0.18 g in F9, F13 and F8 isolates, respectively. According to the results of SEM examinations, it was determined that the isolates carried out calcite accumulation.

Keywords

Biocalcification, Bacillus, 16S rRNA, Urease activity

Supporting Institution

Erciyes Üniversitesi

Project Number

FLY 2014-5560

Thanks

This study was supported by the project No: FLY 2014-5560 from the Erciyes University Scientific Research Projects Unit.

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