DNA Barcoding of Some Lichenized Fungi from Horseshoe Island (Antarctic Peninsula, Antarctica)

Year 2024, Volume: 40 Issue: 2, 315 - 330, 31.08.2024

Merve Yiğit , Mehmet Gökhan Halıcı

Abstract

In this study, it was aimed to identify some lichenized fungi species from Horseshoe Island which is located in the west of the Antarctic Peninsula and where the temporary Turkish Science Base is located, through their morphological and anatomical properties and DNA barcoding using the nrITS or mtSSU gene regions. In this direction, DNA barcoding of samples belonging to Acarospora macrocyclos Vain., Rhizocarpon grande (Flörke ex Flot.) Arnold, Mastodia tessellata (Hook. f. & Harv.) Hook. f. & Harv. and Verrucaria tesselatula Nyl. species which has been collected from Horseshoe Island by the second author were carried out. Within the scope of the study, nrITS data were obtained for the first time for A. macrocylos and R. grande and mtSSU data was obtained for the first time for M. tessellata species and uploaded to genBANK.

Keywords

Antarctica, biodiversity, DNA barcoding, Horseshoe island, Lichenized fungi, nrITS, mtssu

Horseshoe Adası (Antarktika Yarımadası, Antarktika)'ndan Bazı Likenleşmiş Mantarların DNA Barkodlaması

Abstract

Bu çalışmada Antarktika Yarımadası’nın batısında bulunan ve geçici Türk Bilim Üssü’nün bulunduğu Horseshoe Adası’nda yayılış gösteren bazı likenleşmiş mantar türlerinin morfolojik ve anatomik özellikleri aracılığı ile teşhis edilmesi ve nrITS veya mtSSU gen bölgeleri kullanılarak DNA barkodlamalarının yapılması amaçlanmıştır. Bu doğrultuda ikinci yazar tarafından Horseshoe Adası’ndan toplanan Acarospora macrocyclos Vain., Mastodia tessellata (Hook. f. & Harv.) Hook. f. & Harv., Rhizocarpon grande (Flörke ex Flot.) Arnold ve Verrucaria tesselatula Nyl. species türlerine ait örneklerin DNA barkodlamaları gerçekleştirilmiştir. DNA barkodlama verilerinin yanı sıra çalışmada bu türlere ait örneklerin detaylı deskripsiyonları ve fotoğrafları da verilmiştir. Çalışma kapsamında A. macrocylos ve R. grande için ilk defa nrITS verileri elde edilirken, M. tessellata türü için ilk defa mtSSU verileri elde edilmiş ve genBANK’a yüklenmiştir.

Keywords

mtSSU, nrITS, Antarktika, biyoçeşitlilik, likenleşmiş mantar, Horseshoe adası

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