Antifungal Activity Of Lawsonia Inermis L. (Henna) Against Clinical Candida Isolates

Abstract

  Lawsonia inermis L. (henna) is a much branched shrub or small tree, cultivated for its voluable leaves although stem, bark and roots have also been used in traditional medicine for over 9,000 years. In this study,  the antifungal activity of L. inermis was determined against clinical Candida isolates The study was carried out using 192 clinical isolates of Candida from six different species; 135 Candida albicans, 19 Candida parapsilosis, 17 Candida glabrata, 13 Candida tropicalis, 5 Candida krusei, 3 Candida kefyr. The anticandidal activities of L. inermis (henna) as paste form was determined by agar diffusion technique. The highest antifungal activity of henna was obtained against 68 (35.4%) clinical Candida isolates (≥20mm inhibition zone) and moderate activity also detected against 73 (38.0%) isolates (5-15 mm inhibition zone). Fifty-one (26.5%) isolates were resistance (no inhibition zone) to henna paste.

Keywords

• Henna,Candida Species,Antifungal Activity

Antifungal Activity of Lawsonia Inermis L. (Henna) Against Clinical Candida Isolates

Abstract

Kına (Lawsonia inermis L.), yaprakları, sürgünleri ve kökleri 9000 yıldan beri geleneksel tedavi için kullanılan, çok dallı çalı veya küçük ağaç formunda bir bitkidir. Bu çalışmada kınanın, klinik olarak izole edilen Candida türleri üzerine antifungal etkisi araştırılmıştır. Çalışma 6 farklı türe ait 192 klinik izolatla gerçekleştirilmiştir. İzolatların 135’i Candida albicans, 19’u Candida parapsilosis, 17’si Candida glabrata, 13’ü Candida tropicalis, 5’i Candida krusei ve 3’ü Candida kefyr türlerine aittir. Antikandidal aktivite, agar difüzyon tekniğiyle belirlenmiştir. En yüksek aktivite 68 (%35.4) Candida albicans izolatlarında 20mm den büyük inhibisyon zonuyla elde edilmiştir. Orta derecede aktivite ise yine aynı türe ait izolatlarda 73(%38.0) oran ve 5-15 mm inhibisyon zonuyla elde edilmiştir. İzolatlardan % 26’sı kınaya karşı dirençli ( inhibisyon zonu oluşmamış) bulunmuştur.

Keywords

• Henna,Candida Species,Antifungal Etki

References

1. Al-Rubiay K.K., Jaber N.N., Al-Mhaawe B.H. and Alrubaiy L.K., (2008).Antimicrobial efficacy of henna extracts. Oman Med. J., 23, 253-256.
2. Arun P., Purushotham K.G., jayarani J. and Kumari V., (2010). In vitro antibacterial activity and flavonoid contents of Lawsonia inermis (henn)..Int. J. Pharm. Tech. Res., 2, 1178-1181.
3. Babu P.D. and Subhasree R.S. (2009). Anticandidal activity of Lawsonia inermis. Aca. J. Plant Sci., 2, 231-232.
4. Barbieri R, Cooppo E, Marchese A., Daglia M,Sobarzo E., Nabani S.M. 2017. Phytochemicals for human disease: An update on plant-derived compounds antibacterial activity. Microbioogical Research, 196:44-68.
5. Berenji F., Rakhshandeh H. and Ebrahimipour H. 2010. In vitro study of the effects of henna extracts (Lawsonia inermis) on Malassezia species. Jundishapur Journal of Microbiology, 3, 125-128.
6. Borade A.M., Kale B.N. and Shete R.V. 2011. A phytopharmacological review on Lawsonia inermis. International Journal of Pharmacy and Life Sciences, 2, 536-541.
7. Charoensup R., Duangyod T., Palanuvei C. and Ruangrungsi N. 2017. Pharmacognosic specifications and Lawsonecontent of Lawsonia inermis leaves. Pharmocognosy Research, 9 (1): 60-64.
8. Chaudhary G., Goyal S. and Poonia P. 2010. Lawsonia inermis L.: a phytopharmacological review. International Journal of Pharmaceutial Sciences and Drug Resistance, 2(2) 91-98.

9. Chowdhury M.M.H., Kubra K. and Ahmed S.R. 2014. Antimicrobial, phytochemical and toxicological evaluation of Lawsonia inermis extracts against clinical isolates of pathogenic bacteria. Research Journal of Medicinal Plants, 8(4):187-195.
10. Elmanama A.A., Alyazji A.A. and Abu Gheneima N.A. 2011. Antibacterial, antifungal and synergistic effect of Lawsonia inermis, Punica granatum and Hibiscus sabdariffa. Annals Alquds Medicine, 7, 33-41.
11. Jeyaseelan E.C., Jenothiny S., Pathmanathan M.K., Jeyadevan J.P. 2012. Antibacterial activity of sequentially extracted of fruits, flowers and leaves of Lawsonia inermis from Jaffna. Asian Pacific Journal of Tropical Biomedicine, 2(109)798-802.
12. Karpe S.T., Kulkarni S.R., Shaikh S.A. and Manikrao A.M. 2011. Bacteriacidal and bacteriostatic activityof isolated naphoquinone fraction of Lawsonia inermis and synthetic Lawsone on Staphlococcus epidermis. Pharmacologyonline, 2, 156 -163.
13. Muhammad H.S. and Muhammad S. 2005. The use of Lawsonia inermis L (henna) in the management of burn wound infections. African Journal of Biotecnology, 4, 934 -937.
14. Rahiman F. A and Taha R.M. 2011. Plant regeneration and induction of coloured callus from henna (Lawsonia inermis syn. L. alba. Journal of Food and Agricultural Environment, 9, 397-399.
15. Rayavarapu K.A., Kaladhar D.S. and Kumar S. 2011. Evaluation of antimicrobial activity of Lawsonia inermis (Henna) on aquapathogens. Journal of Pharmaceutical and Biomedical Sciences, 7(02)1-3.
16. Saadabi M.A.A. 2007. Evaluation of Lawsonia inermis L: (Sudanase Henna) leaf extracts as an antimicrobial agent. Reseach Journal of Biological Sciences, 2, 419-423.
17. Sharma K.K., Saikia R., Kotoky J., Kalita J.C. and Devl R. 2011. Antifungal activity of Salanum melongena L., Lawsonia inermis and Justica gendarussa against dermotophytes. International Journal of Pharm Tech Research, 3, 1635-1640.
18. Siddiqui B.S., Kardar M.N., Ali S.T. and Khan S. 2003. Two newand known compound from Lawsonia inermis. Helvetica Chimica Acta, 86, 2164-2169.
19. Sowjanya N.C. and Chary C.M. 2012. Degradation of few avean feathers by Microsporum gypseum. Journal of Phytology, 4(4), 21-23.