Assessment of Palynological Characterization and Total Phenol-Flavonoid Content of Some Honeys from Ordu in Turkey

Abstract

Honey is one of the important honey bee products and varies total phenolic and flavonoid contents depending on mainly its floral source. In this study, the total phenolic-flavonoid content and pollen composition of fifteen honey samples were investigated from Ordu in Turkey. According to melissopalynological (qualitative) analysis, eleven honey samples were unifloral (Castanea sativa (9); Rosaceae (1) and Onobrychis spp.(1)), while four samples were multifloral. The forty-two pollen types from the samples were identified. The melissopalynological (quantitative) analysis based on Maurizio’s Class demonstrated that one in Class II, three in Class III, one in Class IV and ten in Class V. Total phenolic and total flavonoid amunts in these honeys varied from 32.5-171.05 mg GAE (gallic acid equivalent)/100g, 1.65-38.75 mg CAE (catechin equivalent)/100g, respectively. According to this study the highest flavonoid levels obtained from Sample 9 (multifloral) and the highest phenolic contents were shown in sample 1 (C. sativa). This study contributes to literature about Ordu honeys.

Keywords

• Flavonoid,honey,phenol,Ordu

Türkiye’nin Ordu iline ait Bazı Balların Palinolojik Karakterizasyonu ve Toplam Fenol-Flavonoid İçeriğinin Değerlendirilmesi

Abstract

Bal, en önemli arı ürünlerinden biridir ve total fenol ve flavonoid miktarı floral kaynağına göre değişkenlik gösterir. Bu çalışmada Türkiye’nin Ordu ilinden alınan 15 bal örneğinin toplam fenol, toplam flavonoid ve polen dağılımı incelenmiştir. Melissopalinolojik (qualitatif) analizlere göre 11 bal unifloral (Castanea sativa (9); Rosaceae (1) and Onobrychis spp.(1)), dört örnek multifloraldır. Örneklerden 42 polen tipi tanımlanmıştır. Maurizio’s Sınıflandırmasına dayanarak yapılan mellissopalinolojik (quantitatif) analizler  1 tane Class II, 3 tane Class III, 1 tane Class IV ve 10 tane Class V olduğunu göstermiştir. Bu ballardaki toplam fenol ve flavonoid içerikleri sırasıyla 32.5-171.05 mg GAE/100g, 1.65-38.75 mg CAE/100g aralığında değişmektedir. Bu çalışmaya göre en yüksek flavonoid seviyesi örnek 9 (multifloral)’dan elde edilmiştir ve en yüksek fenol içeriği örnek 1 (C. sativa)’de gözlenmiştir. Bu çalışma literature Ordu balları hakkında katkı sağlamaktadır.

Keywords

• Ordu,flavonoid,phenol,bal

References

1. Buba F., Gidado A., Shugaba A., (2012). "Analysis of biochemical composition of honey samples from North-East Nigeria", Biochem. Anal. Biochem., 2, 1–7.
2. Chung Y.C., Chang C.T., Chao W.W., Lin C.F., Chou S.T., (2002). "Antioxidative activity and safety of the 50 ethanolic extract from red bean fermented by Bacillus subtilis IMR-NK1", J. Agric. Food Chem., 50, 2454–2458.
3. Kečkeš, S., Gašić, U., Veličković, T.Ć., Milojković-Opsenica, D., Natić, M., Tešić, Ž. (2013). "The determination of phenolic profiles of Serbian unifloral honeys using ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry", Food Chem., 138, 32–40.
4. Khalil, M.I., Moniruzzaman, M., Boukraâ, L., Benhanifia, M., Islam, M.A., Islam, M.N., Gan, S.H., (2012). "Physicochemical and antioxidant properties of Algerian honey", Molecules, 17, 11199–11215.
5. Kolaylı, A., Yazicioğlu, R., Ulusoy, E., S. (2008). "Antioxidant and antimicrobial activities of selected Turkish honeys". Hacettepe Journal of Biology and Chemistry, 36, 163-172.
6. Lachman, J., Orsák, M., Hejtmánková, A., Kovářová, E., (2010). "Evaluation of antioxidant activity and total phenolics of selected Czech honeys", LWT - Food Sci. Technol., 43, 52–58.
7. Lieux, M.H., (1972). "A melissopalynological study of 54 Louisiana (U.S.A.) honeys", Rev. Palaeobot. Palynol., 13, 95–124.
8. Louveaux, J., Maurizio A., Vorwohl G. (1978). Methods of melissopalynology, Bee World, 59, 139–157.

9. Maurizio A., Hodges F. E. D., (1951). Pollen analysis of honey, Bee World, 321–325.
10. Maurizio A. (1975). How bees make honey, A Compr. Surv. honey, 77–105.
11. Meda A., Lamien C.E., Romito M., Millogo J., Nacoulma O.G., (2005). "Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity", Food Chem., 91, 571–577.
12. Michie D., Litterick A., Crews C., (2011). "The influence of outdoor windrow composting on the concentration of grayanotoxins in Rhododendron leaves", Compost Sci. Util., 19, 44–51.
13. Moar N.T., (1985). "Pollen analysis of New Zealand honey", New Zeal. J. Agric. Res., 28, 39–70.
14. Moniruzzaman M., Yung An C., Rao P.V., Hawlader M.N.I., Azlan S.A.B.M., Sulaiman S. A., Gan S.H., (2014). "Identification of phenolic acids and flavonoids in monofloral honey from Bangladesh by high performance liquid chromatography: Determination of antioxidant capacity", Biomed Res. Int.. doi:10.1155/2014/737490.
15. Özbek H., (2011). "Sainfoin, Onobrychis viciifolia Scop.: An Important Bee Plant". Uludağ Bee Journal, 11, 51-62.
16. Pontis J.A., da Costa L.A.M.A., da Silva S.J.R., Flach A., (2014). "Color, Phenolic and Flavonoid Content, and Antioxidant Activity of Honey from Roraima, Brazil", Food Sci. Technol., 34, 69–73.
17. Rababah T. M., Al-Omoush M., Brewer S., Alhamad M., Yang W., Alrababah M., Almajwal A., (2014). "Total phenol, antioxidant activity, flavonoids, anthocyanins and color of honey as affected by floral origin found in the arid and semiarid mediterranean areas", J. Food Process. Preserv., 38, 1119–1128.
18. Ramos I.L.S., Pérez B.M., Ferreras C.G., (2002). "Pollen spectra of different unifloral honeys from La Palma (Canary Islands, Spain)", Grana, 41, 48–57.
19. Sarikaya A.O., Ulusoy E., Öztürk N., Tunçel M., Kolayli S., (2009). "Antioxidant activity and phenolic acid constituents of chestnut (Castania Sativa Mill.) Honey and Propolis", J. Food Biochem., 33, 470–481.
20. Slinkard K., Singleton V., (1977). "Total phenol analysis: automation and comparison with manual methods", Am. J. Enol. Vitic., 28, 49–55.
21. Šarić G., Marković K., Major N., Krpan M., Uršulin-Trstenjak N., Hruškar M., Vahčić N., (2012). "Changes of antioxidant activity and phenolic content in Acacia and multifloral honey during storage", Food Technol. Biotechnol., 50, 434–441.
22. White, J.W., Doner, L.W. (1980). “Honey Composition and Properties: Beekeeping in the United States”. Agriculture Handbook. 335, 82–91.