Histopathology and Antibiotic Susceptibility of Aeromonas hydrophila Isolated from Diseased Guppy (Poecilia reticulata)

Year 2020, , 306 - 313, 20.03.2020

Çiğdem Ürkü

https://doi.org/10.18185/erzifbed.544683

Cited By: 2

Abstract

Abstract This study was carried out to determine the reason of mortality in guppy (Poecilia reticulata) kept in an aquarium fish-rearing farm in Istanbul province and confirm damage caused in tissues and antibiotic susceptibility of isolated pathogen bacteria. Externally diseased guppies (n=15) showed sloughing scales, ulcerative skin lesions on the body surface and abdominal distension; internally pale liver and accumulation of a liquid in the abdominal cavity were observed. Bacteriological inoculation from internal organs such as liver, spleen and kidney of guppies (n=15) were made onto Tryptic Soy Agar (TSA) and Brain Heart Infusion Agar (BHIA). After incubation, all isolated bacteria were identified as Aeromonas hydrophila according to their psychological, morphological, biochemical characteristic, and API 20E profile.  Also, these isolates (n=15) were determined to be sensitive to chloramphenicol and florfenicol. Histopathologically, multifocal liquefactive necrosis in the spleen, liver, kidney and heart, degeneration of tubular epithelium and necrosis in the kidney, desquamation of mucosa epithelium and necrotic gastric glands in the stomach, discharge of the white pulp in the spleen, desquamation of the lamina propria and mucosa epithelium in the intestine, and sloughed off the necrotic gill epithelium cells were observed.

Keywords

Aeromonas hydrophila, antibiotic susceptibility, API 20E, guppy, histopathology

Hasta Lepistes Balığından (Poecilia reticulata)İzole Edilen Aeromonas hydrophila’ nın Antibiyotik Duyarlılığı ve Histopatolojisi

Abstract

Bu çalışma İstanbul ilinde bulunan bir akvaryum balığı
üretim işletmesindeki hasta lepistes balıklarında görülen ölümlerin nedenini
ortaya koymak ve izole edilen patojen bakterinin dokularda oluşturduğu hasarı
ve antibiyotik duyarlılığını tespit etmek amacı ile yürütülmüştür. Eksternal
olarak, ölmek üzere olan lepistes (Poecilia
reticulata) balıklarında pullarda dökülme, vücut yüzeyinde ülseratif deri
lezyonları ve abdominal genişleme görüldü; internal olarak karaciğerin renginde
açılma, abdominal boşlukta sıvı birikimi tespit edildi. Karaciğer, böbrek ve
dalak gibi iç organlardan Tryptic Soy Agar (TSA) and Brain Heart Infusion Agar
(BHIA) besiyerlerine ekimler yapıldı. İnkubasyon sonrasında izole edilen tüm
bakteriler fizyolojik, morfolojik ve biyokimyasal özellikleri ve API 20E
profiline göre Aeromonas hydrophila olarak identifiye edildi. Bu izolatların (n=15) kloramfenikol ve
florfenikol’e duyarlı olduğu tespit edildi. Histopatolojik olarak dalak,
karaciğer, böbrek ve kalp dokusunda multifokal liquefaktif nekroz, tubuler
böbrek epitelyumunda dejenerasyon ve nekroz, mide bezlerinde ve mukoza
epitelyumunda dökülme, dalağın kırmızı pulpasında boşalma, bağırsağın mukoza
epitelinde ve lamina propriasında dökülme yanı sıra nekrotik solungaç epitel
hücrelerinde dökülme tespit edilmiştir.

Keywords

Aeromonas hydrophila, API 20E, antibiyotik duyarlılığı, histopatoloji

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