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KAYNAK ALABALIĞI (SALVELINUS FONTINALIS)’NIN SPERMATOLOJİK ÖZELLİKLERİ VE SPERMANIN KISA SÜRELİ MUHAFAZASI

Year 2015, , 88 - 99, 25.06.2015

Temel Şahin , Özay Köse

https://doi.org/10.18185/eufbed.90287
Cited By: 1

Abstract

Bu çalışma kaynak alabalığının spermatolojik özelliklerini belirlemek ve spermanın kısa süreli muhafazası için sulandırıcı geliştirmek amacıyla yapılmıştır. Üreme sezonunda 6 erkek bireyden sperma abdominal masaj yöntemi ile alınmış ve spermalarda, miktar, motilite, motilite süresi, spermatokrit oranı, yoğunluk ve pH belirlenmiştir. Kaynak alabalığı spermalarında; miktar (ml), motilite (%), motilite süresi (s), spermatokrit oranı (%), yoğunluk (x 109 spermatozoon/ml) ve pH değerleri sırasıyla ortalama 4.3 ± 1.54, 98.3± 4.08, 59.2 ± 17.27, 27.1 ± 4.47, 12.9 ± 1.29 ve 6.9 ± 0.15 bulunmuştur. Sulandırıcı S1’de muhafaza edilen spermanın daha yüksek bir motiliteye sahip olduğu ve sulandırıcı S1’in diğerinden daha etkin olduğu görülmüştür.

Keywords

Kaynak Alabalığı Salvelinus fontinalis Sperma Motilite Sperma özellikleri

References

  • Alavi, S.M. and Cosson, J. 2005. Sperm motility in fishes. I.Effects of temperature and pH: a review. Cell. Biol. İnt, 29(2), 101-10.
  • Alavi, S.M.H., Pšenička, M., Policar, T., Rodina, M., Hamáčková, J., Pavel Kozák, P., Linhart, O. 2009. Sperm quality in male Barbus barbus L. fed different diets during the spawning season. Fish Physiol Biochem, 35, 683-693.
  • Aral, F., Şahinöz, E., Dogu, Z. 2007. A Study on the Milt Quality of Oncorhynchus mykiss (Walbaum, 1972) and Carasobarbus luteus (Heckel, 1843) in Atatürk Dam Lake, Southeastern Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 7, 41-44.
  • Babiak, I., Fraser, L., Dobosz, S., Goryczko, K., Kuzminski, H., Strzezek, J. 1999. Computercontrolled freezing of rainbow trout Onchorhynchus mykiss (Walbaum) spermatozoa for routine programmes. Aquacult. Res, 30, 707-710.
  • Benau, D., Terner, C. 1980. Initiation, prolongation and reactivation of the motility of salmonid spermatozoa. Gamete Res, 3, 247- 257.
  • Billard, R., Cosson, J., Perches, G., Linhart, O. 1995. Biology of sperm and artificial reproduction in carp. Aquaculture, 129, 95-112.
  • Bozkurt, Y. 2004. Aynalı sazan (Cyprinus carpio L.1758) spermasının bazı spermatolojik özelliklerinin belirlenmesi ve farklı sulandırıcılar ile kısa süreli saklanmasının değerlendirilmesi. Doktora Tezi. Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Ankara, Türkiye, 168 sayfa.
  • Bozkurt, Y., Seçer, S., Tekin, N., Akçay, E. 2005. Cryopreservation of rainbow trout (Oncorhynchus mykiss) and mirror carp (Cyprinus carpio) sperm with glucose based extender. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 1(1), 21-25.
  • Bozkurt, Y., Seçer, S., Bekcan, S. 2006a. Relationship between spermatozoa motility, egg size, fecundity and fertilization success in Salmo trutta abanticus. Tarım Bilimleri Dergisi, 12 (4), 345-348.
  • Bozkurt, Y., Seçer, S., Bukan, N., Akçay, E., Tekin, N. 2006b. Relationship between body condition, physiological and biochemical parameters in brown trout (Salmo trutta fario) sperm. Pakistan Journal of Biological Sciences, 9(5), 940-944.
  • Bozkurt, Y., Öğretmen, F., Kökçü, Ö., Ercin, U. 2011. Relationships between seminal plasma composition and sperm quality parameters of the Salmo trutta macrostigma (Dumeril, 1858) semen: with emphasis on sperm motility. Czech J. Anim. Sci., 56(8), 355–364.
  • Büyükhatipoğlu, S. ve Holtz, W. 1977. Preservation of trout sperm in liqiud of frozen state. Aquaculture, 14, 96-56.
  • Canyurt, M.A., Akhan, S. 2008. Effect of ascorbic acid supplementation on sperm quality of rainbow trout (Onchorynchus mykiss). Turkish Journal of Fisheries and Aquatic Sciences, 8, 171-175.
  • Ciereszko, A., Dabrowski, K. 1993. Estimation of spermdensity of rainbow sperctrophotometric technique. Aquaculture, 109, 367-373. whitefish and yellow perch using a
  • Cloud, J.G., Miller, W.H., Levanduski, M.J. 1990. Cryopreservation of sperm as a means to store salmonid germ plasm and to transfer genes from wild fish to hatchery populations. Prog. Fish Cult. 52, 51–53.
  • DeGraaf, J.D., Berlinsky, D.L. 2004. Cryogenic and refrigerated storage of Atlantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) spermatozoa. Aquaculture, 234(1–4), 527–540.
  • Geffen, A.J., Evans, J.P. 2000. Sperm traits and fertilization success of male and sex reversed female rain bow trout (Oncorhynchus mykiss). Aquaculture, 182, 61-72.
  • Glogowski, J., Kwasnik, M., Piros, B., Dabrowski, K., Goryczko, K., Dobosz, S., Kuzminski, H., Ciereszko, A. 2000. Characterization of rainbow trout milt collected with a catheter: semen parameters and cryopreservation success. Aquaculture Research, 31, 289-296.
  • Hatipoğlu, T., Akçay, E. 2010. Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954). Ankara Üniv Vet Fak Derg, 57, 33-38.
  • Linhart, O., Rodina, M., Gela, D., Kocour, M. 2004. Optimization of artificial propagation in European catfish, Silurus glanis L. Aquaculture, 235, 619–632.
  • Liu, L., Wei, Q., Guo, F., Zhang, J. and Zhang, T. 2006. Cryopreservation of chinese sturgeon (Acipenser sinensis) sperm. J. Appl. Ichthyol. 22(supp.1), 384-388.
  • Munkıttrıck, K.R., Moccıa, R.D. 1987. Seasonal changes in the quality of Rainbow trout (Salmo gairdneri) semen: effect of a delay in stripping on spermatocrit, motility, volume and seminal plasma contituents. Aquaculture, 64, 147-156.
  • Piironen, J. and Hyvarinen, H. 1983. Composition of milt of some teleost fish. Journal of Fish Biology, 22, 351-361.
  • Rurangwa, E., Kime, D.E., Ollevier, F., Nash, J.P. 2004. The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture, 234, 1-28.
  • Saad, A., Billard, R., Theron, M.C. and Hollebecq, M.G. 1988. Short- therm presevation of carp (Cyprinus carpio) semen. Aquaculture, 71, 133-150.
  • Suquet, M., Billard, R., Cosson, J., Dorange, G., Chauvaud, L., Mugnier, C., Fauvel, C. 1994. Sperm features in turbot (Scophthalmus maximus): a comparison with other freshwater and marine fish species. Aquatic Living Resources, 7, 283-294.
  • Suquet, M., Dreanno, C., Dorange, G., Normant, Y., Quemener, L., Gaignon, J.L., Billard, R. 1998. The aging phenomenon of turbot (Scophthalmus maximus) spermatozoa: effects on morphology, motility and concentration, intracellular ATP content, fertilization and storage capacities. Journal of Fish Biology, 32, 31-41.
  • Şahin, T., Kurtoğlu, İ.Z. ve Köse, Ö. 2013. Gökkuşağı alabalığı (Oncorhynchus mykiss)’nın spermatolojik özellikleri ve spermanın kısa süreli muhafazası. Fırat Unv. Journal of Science, 25(1), 77-92.
  • Tekin, N., Seçer, S., Akçay, E., Bozkurt, Y., Kayam, S. 2003. Gökkuşağı alabalıklarında (Oncorhynchus mykiss W., 1792) yaşın spermatolojik özellikler üzerine etkisi. Türk J Vet Anim Sci, 27, 37–44.
  • Tuset, V.M., Dietrich, G.J., Wojtczak, M., Słowińska, M., de Monserrat, J., Ciereszko, A. 2008. Relationships between morphology, motility and fertilization capacity in rainbow trout (Oncorhynchus mykiss) spermatozoa. J. Appl. Ichthyol, 24, 393–397.
  • DeGraaf, J.D., King, V.W., Benton, C., Berlinsky, D.L. 2004. Production and storage of sperm from the black sea bass Centropristis striata. Aquacult. Res., 35, 1457-1465.

-

Year 2015, , 88 - 99, 25.06.2015

Temel Şahin , Özay Köse

https://doi.org/10.18185/eufbed.90287
Cited By: 1

Abstract

This study was carried out to determine the spermatologic characteristics and to develop an extender for the short-term storage of semen from brook trout. Four different extenders were tested in the study: a) 8.75 g/l NaCl, 0.20 g/l KCl, 0.20 CaCl2, 0.30 g/l NaHCO3 (S1), b) 8.75 g/l NaCl, 0.20 g/l KCl, 0.20 MgCl2, 0.40 g/l NaHCO3 (S2), c) 7.25 g/l NaCl, 0.40 g/l KCl, 0.80 g/l NaHCO3, 2.0 g/l glucose (S3), d) 8.75 g/l NaCl, 0.20 g/l KCl, 0.10 CaCl2, 0.10 g/l MgCl2, 0.40 g/l NaHCO3 (S4). In the spawning season, sperm was collected by abdominal massage from 6 males. In collected sperm; volume, motility, duration of motility, spermatocrit, density and pH were determined. In the sperm of brook trout, volume (ml), motility (%), duration of motility (sec), spermatocrit, density (x 109spermatozoon/ml), and pH values were found as mean 4.3 ± 1.54, 98.3± 4.08, 59.2 ± 17.27, 27.1 ± 4.47, 12.9 ± 1.29 ve 6.9 ± 0.15, respectively. Sperm stored in extender S1 had a higher rate of motility and the extender S1 seemed to be more effective than the others

Keywords

Brook trout Salvelinus fontinalis Sperm Motility Spermatologic characteristics

References

  • Alavi, S.M. and Cosson, J. 2005. Sperm motility in fishes. I.Effects of temperature and pH: a review. Cell. Biol. İnt, 29(2), 101-10.
  • Alavi, S.M.H., Pšenička, M., Policar, T., Rodina, M., Hamáčková, J., Pavel Kozák, P., Linhart, O. 2009. Sperm quality in male Barbus barbus L. fed different diets during the spawning season. Fish Physiol Biochem, 35, 683-693.
  • Aral, F., Şahinöz, E., Dogu, Z. 2007. A Study on the Milt Quality of Oncorhynchus mykiss (Walbaum, 1972) and Carasobarbus luteus (Heckel, 1843) in Atatürk Dam Lake, Southeastern Turkey. Turkish Journal of Fisheries and Aquatic Sciences, 7, 41-44.
  • Babiak, I., Fraser, L., Dobosz, S., Goryczko, K., Kuzminski, H., Strzezek, J. 1999. Computercontrolled freezing of rainbow trout Onchorhynchus mykiss (Walbaum) spermatozoa for routine programmes. Aquacult. Res, 30, 707-710.
  • Benau, D., Terner, C. 1980. Initiation, prolongation and reactivation of the motility of salmonid spermatozoa. Gamete Res, 3, 247- 257.
  • Billard, R., Cosson, J., Perches, G., Linhart, O. 1995. Biology of sperm and artificial reproduction in carp. Aquaculture, 129, 95-112.
  • Bozkurt, Y. 2004. Aynalı sazan (Cyprinus carpio L.1758) spermasının bazı spermatolojik özelliklerinin belirlenmesi ve farklı sulandırıcılar ile kısa süreli saklanmasının değerlendirilmesi. Doktora Tezi. Ankara Üniversitesi, Fen Bilimleri Enstitüsü, Ankara, Türkiye, 168 sayfa.
  • Bozkurt, Y., Seçer, S., Tekin, N., Akçay, E. 2005. Cryopreservation of rainbow trout (Oncorhynchus mykiss) and mirror carp (Cyprinus carpio) sperm with glucose based extender. Süleyman Demirel Üniversitesi Eğirdir Su Ürünleri Fakültesi Dergisi, 1(1), 21-25.
  • Bozkurt, Y., Seçer, S., Bekcan, S. 2006a. Relationship between spermatozoa motility, egg size, fecundity and fertilization success in Salmo trutta abanticus. Tarım Bilimleri Dergisi, 12 (4), 345-348.
  • Bozkurt, Y., Seçer, S., Bukan, N., Akçay, E., Tekin, N. 2006b. Relationship between body condition, physiological and biochemical parameters in brown trout (Salmo trutta fario) sperm. Pakistan Journal of Biological Sciences, 9(5), 940-944.
  • Bozkurt, Y., Öğretmen, F., Kökçü, Ö., Ercin, U. 2011. Relationships between seminal plasma composition and sperm quality parameters of the Salmo trutta macrostigma (Dumeril, 1858) semen: with emphasis on sperm motility. Czech J. Anim. Sci., 56(8), 355–364.
  • Büyükhatipoğlu, S. ve Holtz, W. 1977. Preservation of trout sperm in liqiud of frozen state. Aquaculture, 14, 96-56.
  • Canyurt, M.A., Akhan, S. 2008. Effect of ascorbic acid supplementation on sperm quality of rainbow trout (Onchorynchus mykiss). Turkish Journal of Fisheries and Aquatic Sciences, 8, 171-175.
  • Ciereszko, A., Dabrowski, K. 1993. Estimation of spermdensity of rainbow sperctrophotometric technique. Aquaculture, 109, 367-373. whitefish and yellow perch using a
  • Cloud, J.G., Miller, W.H., Levanduski, M.J. 1990. Cryopreservation of sperm as a means to store salmonid germ plasm and to transfer genes from wild fish to hatchery populations. Prog. Fish Cult. 52, 51–53.
  • DeGraaf, J.D., Berlinsky, D.L. 2004. Cryogenic and refrigerated storage of Atlantic cod (Gadus morhua) and haddock (Melanogrammus aeglefinus) spermatozoa. Aquaculture, 234(1–4), 527–540.
  • Geffen, A.J., Evans, J.P. 2000. Sperm traits and fertilization success of male and sex reversed female rain bow trout (Oncorhynchus mykiss). Aquaculture, 182, 61-72.
  • Glogowski, J., Kwasnik, M., Piros, B., Dabrowski, K., Goryczko, K., Dobosz, S., Kuzminski, H., Ciereszko, A. 2000. Characterization of rainbow trout milt collected with a catheter: semen parameters and cryopreservation success. Aquaculture Research, 31, 289-296.
  • Hatipoğlu, T., Akçay, E. 2010. Fertilizing ability of short-term preserved spermatozoa Abant trout (Salmo trutta abanticus T, 1954). Ankara Üniv Vet Fak Derg, 57, 33-38.
  • Linhart, O., Rodina, M., Gela, D., Kocour, M. 2004. Optimization of artificial propagation in European catfish, Silurus glanis L. Aquaculture, 235, 619–632.
  • Liu, L., Wei, Q., Guo, F., Zhang, J. and Zhang, T. 2006. Cryopreservation of chinese sturgeon (Acipenser sinensis) sperm. J. Appl. Ichthyol. 22(supp.1), 384-388.
  • Munkıttrıck, K.R., Moccıa, R.D. 1987. Seasonal changes in the quality of Rainbow trout (Salmo gairdneri) semen: effect of a delay in stripping on spermatocrit, motility, volume and seminal plasma contituents. Aquaculture, 64, 147-156.
  • Piironen, J. and Hyvarinen, H. 1983. Composition of milt of some teleost fish. Journal of Fish Biology, 22, 351-361.
  • Rurangwa, E., Kime, D.E., Ollevier, F., Nash, J.P. 2004. The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture, 234, 1-28.
  • Saad, A., Billard, R., Theron, M.C. and Hollebecq, M.G. 1988. Short- therm presevation of carp (Cyprinus carpio) semen. Aquaculture, 71, 133-150.
  • Suquet, M., Billard, R., Cosson, J., Dorange, G., Chauvaud, L., Mugnier, C., Fauvel, C. 1994. Sperm features in turbot (Scophthalmus maximus): a comparison with other freshwater and marine fish species. Aquatic Living Resources, 7, 283-294.
  • Suquet, M., Dreanno, C., Dorange, G., Normant, Y., Quemener, L., Gaignon, J.L., Billard, R. 1998. The aging phenomenon of turbot (Scophthalmus maximus) spermatozoa: effects on morphology, motility and concentration, intracellular ATP content, fertilization and storage capacities. Journal of Fish Biology, 32, 31-41.
  • Şahin, T., Kurtoğlu, İ.Z. ve Köse, Ö. 2013. Gökkuşağı alabalığı (Oncorhynchus mykiss)’nın spermatolojik özellikleri ve spermanın kısa süreli muhafazası. Fırat Unv. Journal of Science, 25(1), 77-92.
  • Tekin, N., Seçer, S., Akçay, E., Bozkurt, Y., Kayam, S. 2003. Gökkuşağı alabalıklarında (Oncorhynchus mykiss W., 1792) yaşın spermatolojik özellikler üzerine etkisi. Türk J Vet Anim Sci, 27, 37–44.
  • Tuset, V.M., Dietrich, G.J., Wojtczak, M., Słowińska, M., de Monserrat, J., Ciereszko, A. 2008. Relationships between morphology, motility and fertilization capacity in rainbow trout (Oncorhynchus mykiss) spermatozoa. J. Appl. Ichthyol, 24, 393–397.
  • DeGraaf, J.D., King, V.W., Benton, C., Berlinsky, D.L. 2004. Production and storage of sperm from the black sea bass Centropristis striata. Aquacult. Res., 35, 1457-1465.
There are 31 citations in total.

Details

Primary Language Turkish
Journal Section Makaleler
Authors

Temel Şahin

Özay Köse

Publication Date June 25, 2015
Published in Issue Year 2015

Cite

APA Şahin, T., & Köse, Ö. (2015). KAYNAK ALABALIĞI (SALVELINUS FONTINALIS)’NIN SPERMATOLOJİK ÖZELLİKLERİ VE SPERMANIN KISA SÜRELİ MUHAFAZASI. Erzincan University Journal of Science and Technology, 8(1), 88-99. https://doi.org/10.18185/eufbed.90287

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Spermatologic characteristics and sperm motility alterations caused by short-term copper exposure in Brook Trout Salvelinus fontinalis
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https://doi.org/10.1080/15569543.2018.1528466