Investigation of SiO2 Nanoparticles Induced Toxicity in Larvae of Fruit Flies

Year 2018, Volume: 11 Issue: 2, 255 - 262, 31.08.2018

Çağla Ersöz Deniz Altun Çolak

https://doi.org/10.18185/erzifbed.412920

Cited By: 1

Abstract

Silicon dioxide (SiO₂)
nanoparticles (NPs) are widely used in many important areas of our lives such
as agriculture, textile, electronics, cosmetics, paint industry, and medicine.
NPs cause toxic effects in various tissues and organs of the body by entering
different ways. The aim of this study is to evaluate the possible toxic effects
of SiO₂ NP (20- 55 nm), among the commonly used NPs, on 3^rd
instar larvae of Drosophila melanogaster.
For this purpose, controlled experiments were carried out to fruit fly larvae
at different time intervals by exposed at 0.1, 1, 5 and 10 mg/mL concentrations
SiO₂ NP. Statistical analysis of the values of the control and
treatment groups was performed with SPSS (version 15.0). According to the
results obtained in the study, when all concentrations compared to the control
it was determined that SiO₂ NPs applied to the larvae increased the
mortality and decreased the recovery of pupae and emergence of adults.

Keywords

Drosophila melanogaster, SiO2 NP, Toxicity, Larval mortality, Lifespan rate

Meyve Sineği Larvalarında SiO2 Nanopartikülünün Toksisite Değerlendirmesi

Abstract

Silisyum dioksit (SiO₂)
nanopartiküllerinin (NP’lerinin) tarım, tekstil, elektronik, kozmetik, boya
endüstrisi ve tıp gibi hayatımızın pek çok önemli alanında yaygın olarak
kullanıldığı bilinmektedir. NP’ler vücuda farklı yollardan girerek değişik doku
ve organlarda toksik etkiye neden olmaktadır. Bu çalışmanın amacı, sık
kullanılan NP’ler arasında yer alan SiO₂ NP’nin (20- 55 nm) Drosophila melanogaster'in 3. evre
larvaları üzerine olası toksik etkileri değerlendirilmiştir. Bu amaçla, meyve
sineği larvalarına 0.1, 1, 5 ve 10 mg/mL konsantrasyonlarda SiO₂
NP’leri uygulanarak farklı saat aralıklarında kontrollü deneyler yapılmıştır.
Kontrol ve uygulama gruplarına ait değerlerin istatistiksel analizi SPSS
(version 15.0) ile yapılmıştır. Çalışma sonucunda elde edilen verilere göre,
larvalara uygulanan SiO₂ NP’lerinin mortaliteyi tüm
konsantrasyonlarda kontrole göre arttırdığı, pupa ve ergin birey oluşumunu ise
azalttığı belirlenmiştir.

Keywords

Drosophila melanogaster, SiO2 NP, Toksisite, Larval mortalite, Yaşama oranı

References

• Ahamed, M. 2013. Silica Nanoparticles-Induced Cytotoxicity, Oxidative Stress and Apoptosis in Cultured A431 and A549 Cells. Human and Experimental Toxicology, 32(2), 186-195.
• Ahmad, J., Ahamed, M., Akhtar, M.J., Alrokayan, S.A., Siddiqui, M.A., Musarrat, J., Al-Khedhairy, A.A. 2012. Apoptosis Induction by Silica Nanoparticles Mediated Through Reactive Oxygen Species in Human Liver Cell Line HepG2. Toxicology and Applied Pharmacology, 259(2), 160-168.
• Akhtar, M.J., Ahamed, M., Kumar, S., Siddiqui, H., Patil, G., Ashquin, M., Ahmad, I. 2010. Nanotoxicity of Pure Silica Mediated Through Oxidant Generation Rather Than Glutathione Depletion in Human Lung Epithelial Cells. Toxicology, 276(2), 95-102.
• Alaraby, M., Annangi, B., Marcos, R., Hernández, A. 2016. Drosophila melanogaster as a Suitable in vivo Model to Determine Potential Side Effects of Nanomaterials: A Review. Journal of Toxicology and Environmental Health, Part B, 19(2), 65-104.
• Atlı Şekeroğlu, Z. 2013. From Nanotechnology to Nanogenotoxicology: Genotoxic Effect of Cobalt-Chromium Nanoparticles”, Turkish Bulletin of Hygiene and Experimental Biology, 70(1), 33-42.
• Dağlıoğlu, Y., Yılmaz Öztürk, B. 2018. Effect of Concentration and Exposure Time of ZnO-TiO2 Nanocomposite on Photosynthetic Pigment Contents, ROS Production Ability, and Bioaccumulation of Freshwater Algae (Desmodesmus multivariabilis). Caryologia, 71(1), 13-23.
• Dağlıoğlu, Y., Yılmaz, H.Ö. ve Yılmaz, O. (2018). Memeli Tümör ve Normal Hücre Hatlarında Nanopartikül Uygulamaları. Arşiv Kaynak Tarama Dergisi, 27(2), 1-1.
• Demir, E., Aksakal, S., Turna, F., Kaya, B. and Marcos, R. 2015. In vivo Genotoxic Effects of Four Different Nano-Sizes Forms of Silica Nanoparticles in Drosophila melanogaster. Journal of Hazardous Materials, 283, 260-266.
• Demir, E. 2016. Nanomateryallerin Toksisite ve Genotoksisite Çalışmalarında Bir in vivo Model Organizma Olarak Drosophila melanogaster (Meyve sineği)’in Kullanılması. Türk Bilimsel Derlemeler Dergisi, 9(1), 1-11.
• Doak, S.H., Griffiths, S.M., Manshian, B., Singh, N., Williams, P.M., Brown, A. P., Jenkins, G.J.S. 2009. Confounding Experimental Considerations in Nanogenotoxicology. Mutagenesis, 24(4), 285-293.
• Donaldson, K, Poland, C.A., Schins R.P.F. 2010. Possible Genotoxic Mechanisms of Nanoparticles: Criteria for Improved Test Strategies. Nanotoxicology, 4(4), 414-420.
• Duan, J., Yu, Y., Li, Y., Yu, Y., Li, Y., Zhou, X., Sun, Z. 2013. Toxic Effect of Silica Nanoparticles on Endothelial Cells through DNA Damage Response via Chk1-Dependent G2/M Checkpoint. PloS One, 8(4), e62087.
• Galal, O.A., El-Samahy, M.F.M. 2016. Genetical Effects of Using Silica Nanoparticles as Biopesticide on Drosophila melanogaster. Egyptian Journal of Genetics and Cytology, 41(1), 87-106.
• Güneş, E., Erdal, M.O., and Gemi, L. 2017. The Effect of Nanofiber on the Biological Traits of Drosophila melanogaster. Sakarya University Journal of Science, 21(6), 1609-1613.
• Ivanov, S., Zhuravsky, S., Yukina, G., Tomson, V., Korolev, D., Galagudza, M. 2012. In vivo Toxicity of Intravenously Administered Silica and Silicon Nanoparticles. Materials, 5(10), 1873-1889.
• Kaewamatawong, T., Shimada, A., Okajima, M., Inoue, H., Morita, T., Inoue, K., Takano, H. 2006. Acute and Subacute Pulmonary Toxicity of Low Dose of Ultrafine Colloidal Silica Particles in Mice After Intratracheal Instillation. Toxicologic Pathology, 34(7), 958-965.
• Kharisov, B.I., Kharissova, O.V., Méndez, U.O. 2014. Nanomaterials on the Basis of Chelating Agents, Metal Complexes, and Organometallics for Environmental Purposes. Nanomaterials for Environmental Protection, 109-124.
• Kim, J.H., Kim, C.S., Ignacio, R.M.C., Kim, D.H., Sajo, M.E.J., Maeng, E.H., Lee, K.J. 2014. Immunotoxicity of Silicon Dioxide Nanoparticles with Different Sizes and Electrostatic Charge. International Journal of Nanomedicine, 9(2), 183-193.
• Kuzma, J., Priest, S. 2010. Nanotechnology, Risk, and Oversight: Learning Lessons from Related Emerging Technologies. Risk Analysis, 30(11), 1688-1698.
• Niu, M., Zhong, H., Shao, H., Hong, D., Ma, T., Xu, K., Sun, J. 2016. Shape-Dependent Genotoxicity of Mesoporous Silica Nanoparticles and Cellular Mechanisms. Journal of Nanoscience and Nanotechnology, 16(3), 2313-2318.
• Özkan, Y., Altinok, I., Ilhan, H., Sokmen, M. 2016. Determination of TiO2 and AgTiO2 Nanoparticles in Artemia salina: Toxicity, Morphological Changes, Uptake and Depuration. Bulletin of Environmental Contamination and Toxicology, 96(1), 36-42.
• Pandey, A., Chandra, S., Chauhan, L.K.S., Narayan, G., Chowdhuri, D.K. 2013. Cellular Internalization and Stress Response of Ingested Amorphous Silica Nanoparticles in the Midgut of Drosophila melanogaster. Biochimica et Biophysica Acta (BBA), General Subjects, 1830(1), 2256-2266.
• Park, E.J., Park, K. 2009. Oxidative Stress and Pro-İnflammatory Responses Induced by Silica Nanoparticles in vivo and in vitro. Toxicology Letters, 184(1), 18-25.
• Parveen, A., Rizvi, S.H.M., Gupta, A., Singh, R., Ahmad, I., Mahdi, F., Mahdi, A.A. 2012. NMR-Based Metabonomics Study of Sub-Acute Hepatotoxicity Induced by Silica Nanoparticles in Rats After Intranasal Exposure. Cellular and Molecular Biology, 58(1), 196-203.
• Passagne, I., Morille, M., Rousset, M., Pujalté, I., L’azou, B. 2012. Implication of oxidative stress in size-dependent toxicity of silica nanoparticles in kidney cells. Toxicology, 299(2-3), 112-124.
• Shamsi, A., Ahmed, A., Bano, B. 2017. Structural Transition of Kidney Cystatin Induced by Silicon Dioxide Nanoparticles: An Implication for Renal Diseases. International Journal of Biological Macromolecules, 94(Pt B), 754-761.
• Sun, L., Li, Y., Liu, X., Jin, M., Zhang, L., Du, Z. and Sun, Z. 2011. Cytotoxicity and Mitochondrial Damage Caused by Silica Nanoparticles. Toxicology In Vitro, 25(8), 1619-1629.
• Vance, M.E., Kuiken, T., Vejerano, E.P., McGinnis, S.P., Hochella Jr, M.F., Rejeski, D., Hull M.S. 2015. Nanotechnology in the Real World: Redeveloping the Nanomaterial Consumer Products Inventory. Beilstein Journal of Nanotechnology, 6, 1769-1780.
• Yektadoost, E., Sari, S., Attar, F., Falahati, M. 2016. An in vitro Study on The Damage of Cell Membrane by Silica Oxide Nanoparticles. Journal of Pharmaceutical and Health Sciences, 4(3), 223-226.
• Yu, X., Hong, F., Zhang, Y.Q. 2016. Bioeffect of Nanoparticles in the Cardiovascular System. Journal of Biomedical Materials Research Part A, 104(11), 2881-2897.
• Yu, J., Yin, W., Peng, T., Chang, Y.N., Zu, Y., Li, J., Zhao, Y. 2017. Biodistribution, Excretion, and Toxicity of Polyethyleneimine Modified NaYF 4: Yb, Er Upconversion Nanoparticles in Mice via Different Administration Routes. Nanoscale, 9(13), 4497-4507.
• Xie, G., Sun, J., Zhong, G., Shi, L., Zhang, D. 2010. Biodistribution and Toxicity of İntravenously Administered Silica Nanoparticles in Mice. Archives of Toxicology, 84(3), 183-190.