INVESTIGATION OF AMOUNTS OF A, E, C VITAMINS WITH MALONDIALDEHYDE AND GLUTATHIONE IN PLANT GUNDELIA TOURNEFORTII

Year 2014, Volume: 7 Issue: 2, 159 - 168, 11.01.2015

Fikret Karataş

https://doi.org/10.18185/eufbed.44500

Cited By: 7

Abstract

In this study, the amounts of vitamin A, vitamin E, vitamin C with malondialdehyde (MDA) and reduced form glutathione (GSH), oxidized form glutathione (GSSG) in Gundelia Tournefortii sample have been determined by using High Performance Liquid Chromatography. The amount of vitamin A, vitamin E, vitamin C with MDA and GSH, GSSG in Gundelia Tournefortii have been obtained to be 0,98 ± 0,22 μg/g; 0,35 ± 0,09 μg/g; 19,72 ± 2,16 μg/g ; 3,84 ± 0,56 μg/g; 262,87 ± 20,73 μg/g and 2,03 ± 0,90 μg/g respectively. From the results obtained as a result of plant Gundelia Tournefortii, vitamin C and reduced glutathione (GSH) have been seen to be rich. If MDA level is low however may indicate that the plant is not under stress. In addition, Gundelia Tournefortii plant, stress-free and watersoluble antioxidants can be said that a plant rich.

Keywords

Gundelia Tournefortii, vitamins, MDA, Glutathione

KENGER (GUNDELİA TOURNEFORTİİ) BİTKİSİNDEKİ A, E, C VİTAMİNLERİ İLE MALONDİALDEHİT VE GLUTATYON MİKTARLARININ ARAŞTIRILMASI

Abstract

 Bu çalışmada, Kenger (Gundelia Tournefortii) bitkisindeki A vitamini, E vitamini, C vitamini ile Malondialdehit (MDA) ve İndirgenmiş glutatyon (GSH), Yükseltgenmiş glutatyon (GSSG) miktarları Yüksek Performanslı Sıvı Kromatografisi (HPLC)  ile belirlendi. Kenger bitkisindeki A, E,  C vitaminleri ile MDA ve GSH, GSSG miktarları sırası ile 0,98 ± 0,22 μg/g; 0,35 ± 0,09 μg/g; 19,72 ± 2,16 μg/g; 3,84 ± 0,56 μg/g; 262,87 ± 20,73 μg/g ve 2,03 ± 0,90 μg/g olduğu gözlendi. Sonuç olarak elde edilen bulgulardan Gundelia Tournefortii bitkisinin, C vitamini ve redükte glutatyon (GSH) açısından zengin olduğu görülmektedir. MDA miktarının düşük olması ise, bitkinin stres altında olmadığının göstergesi olabilir. Ayrıca Gundelia Tournefortii bitkisinin stressiz ve suda çözünen antioksidanlar açısından zengin bir bitki olduğu söylenebilir.

Keywords

Gundelia Tournefortii, vitaminler, MDA ve Glutatyon

References

• Akan, H., Korkut, M.M. ve Balos, M.M. (2008). An ethnobotanical study
• around Arat Mountain and its surroundings (Birecik, Sanlıurfa).
• Fırat University Journal of Science and Engineering., 20, 67-81.
• Aksoy, M. (2000). Beslenme Biyokimyası. Hatipoğlu Basım ve Yayım San. Tic.
• Ltd. Şti., 321 342, 564-565s, Ankara.
• Asadi-Samani, M., Rafieian-Kopaei, M., Azimi, N. (2013). Gundelia: A systematic review of medicinal and molecular perspective. Pakistan journal of biological sciences: PJBS 16 (21), 1238-1247.
• Azeez, O. H. and Kheder, A. E. (2012). Effect of Gundelia tournefortii on some
• biochemical parameters in dexamethasone-induced hyperglycemic
• and hyperlipidemic mice. Iraqi Journal of Veterinary Sciences, Vol. 26, No. 2, 73-79
• Büyükakyüz, N., Altuğ, T. ve Yaltırık, M. (2000). Kanser proflaksisinde antioksidan maddelerden E vitamini ve selenyumun önemi. Diş Hekimliğinde Klinik Derg., 12, 136-139.
• Cakmakci, S. ve Dagdemir, E. (2013). A preliminary study on functionality of Gundelia tournefortii L. as a new stabiliser in ice cream production. International Journal of Dairy Technolog, 66 (3), 431–436.
• Chai, Y.C., Ashraf, S.S., Rokutan, K., Johnston, R.B. ve Jr, Thomas, J.A. (1994). Sthiolation of individual human neutrophil proteins including actin by stimulation of the respiratory burst: evidence againist a role for glutathione disulfide. Arch Biochem Biophys, 310, 273-281.
• Cheesman, K.H. ve Slater, T.F. (1993). Introduction to free radical biochemistry. Br Med Bull, 49, 481-493.
• Çoruh, N., Sağdıçoğlu Celep, A.G., Özgökçe, F. and İşçan, M. (2007). Antioxidant capacities of Gundelia tournefortii L. extracts and inhibition on glutathione-S-transferase activity. Food Chem. 100, 1249–1253.
• Dawes, P. ve Dawes, E. (2000). SGE Choromatography Products Catalog. pg: 182.
• El-Demerdash, F.M., Yousef, M.I. ve Kedwany, F.S. (2004). Cadmium- induced changes in lipid peroxidation, blood hematology, biochemical parameters and semen guality of male rats, protective role of vitamin E and carotene. Food and Chemical Toxicology, 42, 1562-1571.
• Esterbauer, H., Gebicki, J., Puhl, H. ve Jürgens, G. (1992). The role of lipid peroxidation and antioxidants in oxidative modification of LDL. Free Rad. Bio. and Med., 13, 341-390.
• Evelson, P., Ordonez, C.P., Llesuy, S. ve Boveris, A. (1997). Oxidative stres and in vivo chemiluminescence in Mouse skin exposed to UVA radiation. J.Photochem Photobia. B, 38 (2-3), 215-219.
• Gonzalez, M.J., Miranda-Massari, J.R., Mora, E.M., Guzman, A., Riordan, N.H., Riordan, H.D., Casciari, J.J., Jackson, J.A. ve Roman-Franco, A. (2005). Orthomolecular oncology review: ascorbic acid and cancer 25 years later. Integrative Cancer Therapies, 4, 32-44.
• Granado, F., Olmedilla, B., Gil-Martinez, E., Blanco, I., Millan, I. ve Rojas- Hidalgo, E. (1998). Carotenoids, retinol and tocopherols in patients with insulin-dependent diabetes mellitus and their immediate relatives. Clinical Science (Colch), 94, 189-195.
• Jialal, I. ve Fuller, C.J. (1993). Oxidized LDL and antioxidants. Clin. Cardiol, 16, 16-19.
• Karatas, F., Karatepe, M. ve Baysar, A. (2002). Determination of free malondialdehyde in human serum by high performence liquid chromatography. Anal Biochemistry, 311, 76-79.
• Karatas, F., Öbek, E. ve Kamışlı, F. (2009). Antioxidant capacity of Lemna gibba L. exposed to wastewater treatment. J. Ecol. Eng., 35, 1225– 1230.
• Karataş, F. (2013). Kuzukulağı (Rumex acetosella L.) bitkisinin A, E ve C vitamini içeriğinin belirlenmesi. SDÜ Fen Bilimleri Enstitüsü Dergisi, 17 (1), 60-63.
• Karataş, F., Bektaş, İ., Birişik, A., Aydın, Z. ve Kurtul, A. (2011). Çiriş Otu’nda (Asphodelus aestivus L.) suda çözünen bazı bileşiklerin araştırılması. SDÜ Journal of Science (E-Journal), 6 (1), 35-39.
• Kılınç, K. (1985). Oksijen radikalleri, üretilmeleri, fonksiyonları, toksik etkileri. Biyokimya Dergisi, 10, 60-89.
• Kocsy, G., Galiba, G. ve Brunold, C. (2001). Role of glutathione in adaptation and signalling during chilling and cold acclimation in plants. Physiol Plantarum, 113, 158–164.
• Konukoğlu, D. ve Akçay, T. (1995). Glutatyon metabolizması ve klinik önemi. Türkiye Klinikleri, Journal Med Science, 15 (4), 214-218.
• Matthäus, B. ve Özcan, M.M. (2011). Chemical evaluation of Flower bud and oils of tumbleweed (Gundelia Tourneforti L.) as a new potential nutrition sources. Journal of Food Biochemistry, 35, 1257–1266.
• Miller, K.W., Lorr, N.A. ve Yang, C.S. (1984). Simultaneous determination of plazma retinol α-tocoferol, lycopene, α-carotene, and β-carotene by high performance liquid chromatography. Analytical Biochemistry, 138, 340-345.
• Morel, Y. ve Barouki, R. (1999). Repression of gene expression by oxidative stress. Biochem J, 342, 481–496.
• Munzuroğlu, Ö., Karataş, F. ve Gür, N. (2000). Işgın (Rheum ribes L.) bitkisindeki A, E ve C vitaminleri ile selenyum düzeylerinin araştırılması. Türk J Biol, 24, 397–404.
• Nikolai, E.P., Tatyana, V.L., Tatyana, A.K. ve Lowell, P.K. (2001). Carotenoids as scavengers of free radicals in a Fenton reaction: Antioxidants or pro-oxidants? Free Rad. Bio. and Med., 31(3), 398- 404.
• Packer, L. (1991). Protective role of vit E in biological systems. Am. J. Clin. Nutr., 53, 1050- 1055.
• Polat, R., Çakılcıoğlu, U., Ertuğ, F. ve Satıl, F. (2012). An evaluation of ethnobotanical studies in Eastern Anatolia. Biological Diversity and Conservation, 5(2), 23-40.
• Sies, H., Stahl, W. ve Sundqust, A.R. (1992). Antioxidant function of vitamins. Vitamin E and C, betacarotene and other carotenoidsa. Ann N Y Acad Sci, 669, 7-20.
• Supelco Chromatography Products for Analysis & Purification (2005-2006) Sigma- Aldrich Chemie GmbH, Export Department Eschenstraße Taufkirchen, 169s, Germany.
• Tabibian, M., Nasri, S., Kerishchi, P., Amin, G. (2013). The Effect of Gundelia Tournefortii Hydro-Alcoholic Extract on Sperm Motility and Testosterone Serum Concentration in Mice. Zahedan J Res Med Sci, 15(8); 18-21
• Tavazzi, B., Lazzarino, G., Di-Pierro, D. ve Giardina, B. (1992). Malondialdehyde production and ascorbate decrease are associated to the eperfusion of the isolated postischemic rat heart. Free Rad. Bio. and Med.,, 13, 75-78.
• Van-Der-Meulen, J.H., McArdle, A., Jackson, M.J. ve Faulkner, J.A. (1997). Contraction-induced injury to the extensor digitorum longus muscles of rats: the role of vitamin E. J. Appl. Physiol, 83 (3), 817- 823.
• Wang, W.X., Vinocur, B. ve Altman, A. (2003). Plant responses to drought, salinity and extreme temperatures: towards genetic engineering for stress tolerance. Planta, 218, 1-14.