Antimicrobial Activity and Chemical Composition of Essential Oil Extracted from Hyoscyamus niger L. Inflorescence

Year 2024, Volume: 17 Issue: 3, 751 - 760, 31.12.2024

Sefa Gözcü , Zeynep Akşit , Hüseyin Akşit

https://doi.org/10.18185/erzifbed.1522296

Abstract

This study aimed to analyze the chemical composition and evaluate the antibacterial activity of essential oils extracted from the H. niger inflorescence collected from Erzincan, Türkiye. The essential oils were extracted using hydrodistillation and analyzed by GC-MS, identifying 23 components, with major constituents being 10-heneicosene (35.72%), phytol (20.50%), and acetic acid, butyl ester (10.10%). The antibacterial activity was tested against six bacterial strains, including Staphylococcus aureus, Clostridium perfringens, Enterococcus faecalis, Bacillus cereus, Listeria monocytogenes, and Escherichia coli, using the disc diffusion method. The H. niger inflorescence essential oil exhibited moderate antibacterial activity, specifically against Clostridium perfringens, with a zone of inhibition of 11.7 ± 1.2 mm compared to the positive control imipenem (17.0±1.0 mm). The results suggest that H. niger inflorescence essential oil has potential as an antibacterial agent, highlighting the importance of exploring plant-derived compounds as alternatives to traditional antibiotics in combating multidrug-resistant bacteria.

Keywords

Hyoscyamus niger L., Essential oil, Antibacterial, GC-MS

Ethical Statement

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Supporting Institution

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Thanks

We thank Professor. Dr. Ali Kandemir for the taxonomic description of the plant samples.

Antimicrobial Activity and Chemical Composition of Essential Oil Extracted from Hyoscyamus niger L. Inflorescence

Abstract

References

• [1] Küpeli Akkol, E., Ilhan, M., Kozan, E., Gürağaç Dereli, F. T., Sak, M., Sobarzo-Sánchez, E. (2020). Insecticidal activity of Hyoscyamus niger L. on lucilia sericata causing myiasis. Plants, 9, 655-665. https://doi.org/10.3390/plants9050655
• [2] Gözcü, S., Korkmaz, M., Çorlu, S., Tuysuz, S. (2024). Traditional uses of medicinal plants in Erzincan province, Türkiye. Journal of Faculty of Pharmacy of Ankara University, 48(1), 34-45. https://doi.org/10.3390/plants905065510.33483/jfpau.1329957
• [3] Ghorbanpour, M., Hatami, M., Hatami, M. (2015). Activating antioxidant enzymes, hyoscyamine and scopolamine biosynthesis of Hyoscyamus niger L. plants with nano-sized titanium dioxide and bulk application. Acta Agriculturae Slovenica, 105(1), 23-32. https://doi.org/10.14720/aas.2015.105.1.03
• [4] Yiğit, B. M., & Gözcü, S. Ethnobotanical study of medicinal plants in ağri province, türkiye: exploring traditional knowledge and therapeutic potential. Journal of Faculty of Pharmacy of Ankara University, 48(2), 486-497. https://doi.org/10.33483/jfpau.1402467.
• [5] Honda, G., Yeşilada, E., Tabata, M., Sezik, E., Fujita, T., Takeda, Y., Tanaka, T. (1996). Traditional medicine in Turkey VI. Folk medicine in West Anatolia: Afyon, Kütahya, Denizli, Muğla, Aydin provinces. Journal of Ethnopharmacology, 53(2), 75-87. https://doi.org/10.1016/S0378-8741(96)01426-2.
• [6] Çırak, C., Kevseroglu, K., Sağlam, B. (2004). Physical and physiological dormancy in black henbane (Hyoscyamus niger L.) seeds. Journal of Plant Biology, 47, 391-395. https://doi.org/10.1007/BF03030556
• [7] Ma, C. Y., Liu, W. K., Che, C. T. (2002). Lignanamides and nonalkaloidal components of Hyoscyamus niger seeds. Journal of natural products, 65(2), 206-209. https://doi.org/10.1021/np010073b
• [8] İnci, Ş., Sancar, P. Y., Demirpolat, A., Kirbag, S., Civelek, S. (2022). Chemical compositions of essential oils, antimicrobial effect and antioxidant activity studies of Hyoscyamus niger L. from Turkey. bioRxiv, 2022(8), 503024. https://doi.org/10.1101/2022.08.07.503024
• [9] Dulger, B., Hacioglu, N., Goncu, B. S., Gucin, F. (2010). Antifungal activity of seeds of Hyoscyamus niger L. (Henbane) against some clinically relevant fungal pathogens. Asian Journal of Chemistry, 22(8), 6321.
• [10] Al-Snafi, A. E. (2018). Therapeutic importance of Hyoscyamus species grown in Iraq (Hyoscyamus albus, Hyoscyamus niger and Hyoscyamus reticulates)-A review. IOSR Journal of Pharmacy, 8(6), 18-32.
• [11] Gandhi, G. R., Hillary, V. E., Antony, P. J., Zhong, L. L., Yogesh, D., Krishnakumar, N. M., & Gan, R. Y. (2024). A systematic review on anti-diabetic plant essential oil compounds: Dietary sources, effects, molecular mechanisms, and safety. Critical reviews in food science and nutrition, 64(19), 6526-6545. https://doi.org/10.1080/10408398.2023.2170320
• [12] Zhang, J., Zhang, M., Bhandari, B., & Wang, M. (2024). Basic sensory properties of essential oils from aromatic plants and their applications: A critical review. Critical Reviews in Food Science and Nutrition, 64(20), 6990-7003. https://doi.org/10.1080/10408398.2023.2177611
• [13] Dhifi, W., Bellili, S., Jazi, S., Bahloul, N., & Mnif, W. (2016). Essential oils’ chemical characterization and investigation of some biological activities: A critical review. Medicines, 3(4), 25-46. https://doi.org/10.3390/medicines3040025
• [14] Ertas, A., Yilmaz, M. A., & Firat, M. (2015). Chemical profile by LC–MS/MS, GC/MS and antioxidant activities of the essential oils and crude extracts of two Euphorbia species. Natural product research, 29(6), 529-534. https://doi.org/10.1080/14786419.2014.954113
• [15] Bakal, S. N., Bereswill, S., Heimesaat, M. M. (2017). Finding novel antibiotic substances from medicinal plants—antimicrobial properties of Nigella sativa directed against multidrug resistant bacteria. European Journal of Microbiology and Immunology, 7(1), 92-98. https://doi.org/10.1556/1886.2017.00001
• [16] Shrivastava, S. R., Shrivastava, P. S., Ramasamy, J. (2018). World health organization releases global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. Journal of Medical Society, 32(1), 76-77. https://doi.org10.4103/jms.jms_25_17
• [17] Gözcü, S., Akşit, Z. Chemical Composition and Antibacterial Activity of Three Volatile Oils Extracted from Nigella sativa L. Seeds. Black Sea Journal of Health Science, 6(4), 662-666. https://doi.org/10.19127/bshealthscience.1318520
• [18] Aksit, H., Bayar, Y., Simsek, S., Ulutas, Y. (2022). Chemical composition and antifungal activities of the essential oils of Thymus species (Thymus pectinatus, Thymus convolutus, Thymus vulgaris) against plant pathogens. Journal of Essential Oil-Bearing Plants, 25(1), 200-207. https://doi.org/10.1080/0972060X.2022.2043189
• [19] Wayne, P., Melvin, P (1999) Performance Standards for Antimicrobial Susceptibility Testing, 9th International Supplement, M100-S9. Wayne, P(Ed), 120-135. National Committee for Clinical Laboratory Standards. Malvern, Pennsylvania, USA.
• [20] Ayari-Guentri, S., Djemouai, N., Gaceb-Terrak, R., Rahmania, F. (2017). Chemical composition and antioxidant activity of Hyoscyamus muticus L. subsp. falezlez (Coss.) Maire from Algeria. Journal of Essential Oil-Bearing Plants, 20(5), 1370-1379. https://doi.org/10.1080/0972060X.2017.1396930
• [21] Mehalaine, S., & Chenchouni, H. (2021). Quantifying how climatic factors influence essential oil yield in wild-growing plants. Arabian Journal of Geosciences, 14(13), 1257. https://doi.org/10.1007/s12517-021-07582-6
• [22] Dulger, B., Goncu, B. S., Gucin, F. (2010). Antibacterial activity of the seeds of Hyoscyamus niger L. (Henbane). Asian Journal of Chemistry, 22(9), 6879-6883.
• [23] Chalabian, F., Majd, A., Mehrabian, S., Falahian, F. (2002). A study of growth inhibitory effect of alkaloids of two species of genus Hyoscyamus on some kinds of microbes of skin. Journal of science (Islamıc Azad University), 12(3), 3371-3378.
• [24] Vanitha, V., Vijayakumar, S., Nilavukkarasi, M., Punitha, V. N., Vidhya, E., Praseetha, P. K. (2020). Heneicosane—A novel microbicidal bioactive alkane identified from Plumbago zeylanica L. Industrial Crops and Products, 154, 112748. https://doi.org/10.1016/j.indcrop.2020.112748
• [25] Ghaneian, M. T., Ehrampoush, M. H., Jebali, A., Hekmatimoghaddam, S., Mahmoudi, M. (2015). Antimicrobial activity, toxicity and stability of phytol as a novel surface disinfectant. Environmental Health Engineering and Management Journal, 2(1), 13-16.
• [26] Pejin, B., Ciric, A., Glamoclija, J., Nikolic, M., Sokovic, M. (2015). In vitro anti-quorum sensing activity of phytol. Natural product research, 29(4), 374-377. https://doi.org/10.1080/14786419.2014.945088
• [27] Pejin, B., Kartali, T., Stanimirović, B., Karaman, M. (2014). Phytol may inspire new medicinal foods for the treatment of heart disease. Asian Journal of Chemistry, 26(23), 8261-8262.
• [28] Bakkali, F., Averbeck, S., Averbeck, D., & Idaomar, M. (2008). Biological effects of essential oils–a review. Food and chemical toxicology, 46(2), 446-475. https://doi.org/10.1016/j.fct.2007.09.106
• [29] Elsharkawy, E. R., Ed-dra, A., Abdallah, E. M., & Ali, A. M. (2018). Antioxidant, antimicrobial and antifeedant activity of phenolic compounds accumulated in Hyoscyamus muticus L. African Journal of Biotechnology, 17(10), 311-321. https://doi.org/10.5897/AJB2017.16316
• [30] Sadeghi, B. (2016). Extraction and comparative identification of essential oil components of different Hyoscyamus species using hydro-distillation and GC-MS. The 23rd Iranian Seminar of Analytical Chemistry, 328
• [31] Karakaya, S., Yilmaz, S. V., Özdemir, Ö., Koca, M., Pınar, N. M., Demirci, B., & Baser, K. H. C. (2020). A caryophyllene oxide and other potential anticholinesterase and anticancer agent in Salvia verticillata subsp. amasiaca (Freyn & Bornm.) Bornm.(Lamiaceae). Journal of Essential Oil Research, 32(6), 512-525. https://doi.org/10.1080/10412905.2020.1813212