Research Article
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ERKEK RATLARDA FLUMETRİN MARUZİYETİNE KARŞI BAİKALİNİN İYİLEŞTİRİCİ ETKİLERİ

Year 2024, , 160 - 167, 25.07.2024
https://doi.org/10.34108/eujhs.1389114

Abstract

Baikalin antioksidan, antiinflamatuar ve antikarsinojenik özelliklere sahip bir flavonoidken, flumetrin bir piretroid insektisittir. Bu çalışmanın amacı, flumetrin ile indüklenmiş ratlarda baikalinin biyokimyasal parametrelere ve lipid peroksidasyonuna etkilerini araştırmaktı. Çalışmada 42 adet rat, her grupta yedi adet rat olacak şekilde altı gruba ayrıldı. Flumetrin ve baikalin mısır yağı içinde tek doz oral gavaj yoluyla 28 gün boyunca verildi: Kontrol grubuna mısır yağı uygulandı. Flumetrin 15 mg/kg c.a. ikinci gruba, flumetrin+baikalin 50 mg/kg c.a. üçüncü gruba, flumetrin+baikalin 100 mg/kg c.a. dördüncü gruba, baikalin 50 mg/kg c.a. beşinci gruba, baikalin 100 mg/kg c.a. altıncı gruba verildi. Daha sonra kan ve doku örnekleri biyokimyasal ve histopatolojik değerlendirmeler için alındı. Elde edilen sonuçlara göre, flumetrin verilen grup kontrol ile karşılaştırıldığında kolesterol, alkalen fosfataz, total protein, üre ve ürik asit düzeyleri anlamlı seviyede azaldı. Ayrıca flumetrin alan grupta böbrek süperoksit dismutaz ve glutatyon peroksidaz ile karaciğer süperoksit dismutaz ve katalaz aktiviteleri azaldı, ancak hem karaciğer hem de böbrek melondialdehit ve nitrik oksit seviyeleri arttı. Flumetrin dokularda histopatolojik değişikliklere neden olmuştur. Öte yandan, baikalin verilen tüm gruplarda istatistiksel olarak böbrek süperoksit dismutaz ve glutatyon peroksidaz ile karaciğer süperoksit dismutaz ve katalaz aktiviteleri arttı, ancak nitrik oksit ve melondialdehit düzeyleri azaldı. Ayrıca baikalin bazı biyokimyasal parametreleri etkiledi (p<0.05) ve doku hasarını azalttı. Elde edilen biyokimyasal sonuçlar histopatolojik sonuçlarla uyumludur. Sonuç olarak, ratlarda flumetrin ile oluşturulan subakut toksikasyonda, baikalin oksidatif stresi ve doku hasarını iyileştirebilir.

Project Number

TYL-2020-9947

References

  • Gajendiran A, Abraham J. An overview of pyrethroidinsecticides. Front Biol. 2018;13(2):79-90.doi:10.1007/s11515-018-1489-z.
  • FAO/WHO report. Pesticideresidues in food-1996. Evaluations. Part I-Residues, Part II-Toxicology. FAO Plant Production and Protection Paper, 140. Roma: Italy. 1996.251-288. https://www.fao.org/publications/card/en/c/c373c3e9-9195-5b59-95b4-36fbe703868f/. AccessedSeptember 10, 2022.
  • EMEA/MRL/469-98, Summary report, committee for veterinary medicinal products, flumethrin. The European Agency for the Evaluation of Medicinal Products. London; United Kingdom.1998.1-7. https://www.ema.europa.eu/en/documents/mrl-report/flumethrin-summary-report-1-committee-veterinary-medicinal-products_en.pdf.AccessedSeptember 10, 2022.
  • Pisoschi AM, Pop A. The role of antioxidants in the chemistry of oxidative stress: A review. Eur J Med Chem. 2015;97(5):55-74. doi:10.1016/j.ejmech.2015.04.040.
  • Karabulut H, Gülay MŞ. Antioksidanlar. MAEU Vet Fak Derg. 2016;1(1):65-76.doi:10.24880/maeuvfd.260790.
  • Jabłońska-Trypuć A. Pesticides as inducers of oxidative stress. Cell Med Press. 2017;3(8):96-110. doi:10.20455/ros.2017.823.
  • Kanbur M, Eraslan G, Soyer SZ, Altınordulu Ş. The effect of Saw palmetto on flumethrin-induced lipid peroxidation in rats. Pestic Biochem Physiol. 2010;97(1):43-46. doi:10.1016/j.pestbp.2009.11.011.
  • Mishra A, Dewangan G, Mahajan V, Mandal TK. Effect of flumethrin on tissue biochemistry following oral administration in wistar albino rats. Int J Pharma Bio Sci. 2012;3(2):191-200.
  • Singh AK, Singh PK, Dey A, Mishra A, Dewangan G, Chakraborty K et al. Effect of flumethrin on hematological and biochemical changes in rats. Explor Anim Med Res. 2012;1(2):131-136.
  • Salama AM, Talkhan OFA, Khattab MS, Fakhry FM. Protective effect of quercetin against oxidative stress, immuno histochemical and histopathological changes induced by flumethrin. Anim HealthRes J. 2019;22(7):191-200.
  • Robards K, Antolovich M. Analytical chemistry of fruit bioflavonoids: a review. The Analyst. 1997;122(2):122-130.doi:10.1039/A606499J
  • Shieh DE, Liu LT, Lin CC. Antioxidant and free radical scavenging effects of baicalein, baicalin and wogonin. Anticancer Res. 2000;20(5A):2861-2865.
  • Chen YC, Chow JM, Lin CW, Wu CY, Shen SC. Baicalein inhibition of oxidative-stress-induced apoptosis via modulation of ERKs activation and induction of HO-1 gene expression in rat glioma cells C6. Toxicol Appl Pharmacol. 2006;16(2):263-273.doi:10.1016/j.taap.2006.05.008.
  • Tu XK, Yang WZ, Shi SS, Wang CH, Chem CM. Neuroprotective effect of baicalin in a rat model of permanent focal cerebral ischemia. Neurochem Res. 2009;34(9):1626-1634.doi:10.1007/s11064-009-9953-4.
  • Yin F, Liu J, Ji X, Wang Y, Zidichouski J, Zhang J. Baicalin prevents the production of hydrogen peroxide and oxidative stress induced by Ab aggregation in SH-SY5Y cells. Neurosci Lett. 2011;492(2):76-79.doi:10.1016/j.neulet.2011.01.055.
  • Hou J, Wang J, Zhang P et al. Baicalin attenuates proinflammatory cytokine production in oxygen-glucose deprived challenged rat microglial cells by inhibiting TLR4 signaling pathway. Int Immunopharmacol. 2012;14(4):749-757.doi:10.1016/j.intimp.2012.10.013.
  • Wang HZ, Wang HH, Huang SS et al. Inhibitory effect of baicalin on collagen-induced arthritis in rats through the nuclear factor-κB pathway. J Pharmacol Exp Ther.2014;350(2):435-443.doi:10.1124/jpet.114.215145.
  • Zhang Q, Sun J, Wang Y et al. Antimycobacterial and anti-inflammatory mechanisms of baicalin via induced autophagy in macrophages Infected with Mycobacterium tuberculosis. Front Microbiol. 2017;8(2):2142.doi:10.3389/fmicb.2017.02142
  • Noh K, Kang Y, Nepal MR, Jeong KS, Oh DG, Kang MJ, Lee S et al. Role of intestinal microbiota in baicalin-induced drug interaction and it's pharmaco kinetics. Molecules. 2016;21(3):337. doi:10.3390/molecules21030337.
  • Xing J, Chen X, Zhong D. Absorption and enterohepatic circulation of baicalin in rats. Life Sci. 2005;78(2):140-146. doi:10.1016/j.lfs.2005.04.072.
  • Kalapos-Kovács B, Magda B, Jani M, Fekete Z, Szabó PT, Antal I et al. Multiple ABC transporters efflux baicalin. Phytother Res. 2015;29(12):1987-1990. doi:10.1002/ptr.5477.
  • Akao T, Kawabata K, Yanagisawa E, Ishihara K, Mizuhara Y, Wakui Y et al. Balicalin, the predominant flavoneglucuronide of scutellariaeradix, is absorbed from the rat gastro intestinal tract as the agly cone and restored to its original form. J PharmPharm. 2000;52(12):1563-1568. doi:10.1211/0022357001777621.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin Phenol Reagent. J Biol Chem. 1951;193(1):265-275. doi:10.1016/S0021-9258(19)52451-6
  • Miller GL. Protein determination of large numbers of samples. Anal Chem. 1959;31(5):964. doi:10.1021/ac60149a611.
  • Yoshioka T, Kawada K, Shimada T, Mori M. Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol. 1979;135(3):372-376.doi:10.1016/0002-9378(79)90708-7.
  • Sun Y, Larry W, Oberley R. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988;34(3):497-500.
  • Tracey WR, Tse J, Carter G. Lipopolysaccharide-induced changes in plasma nitrite and nitrate concentrations in rats and mice: pharmacological evaluation of nitric oxide synthase inhibitors. J Pharmacol Exp Ther. 1995;272(3):1011-1015.
  • Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967;70(1):158-169.
  • Luck H. Catalase. IN: Methods of enzymatic analysis. (Bergmeyer HU. Ed.) New York: Academic Press; 1971;3(7A):885-893.
  • Küçükkurt İ, İnce S, Aytekin İ, Birdane YO. The effects of flumethrin and flumethrin+vitamin C application on oxidative stress biomakers in chios sheep. Kocatepe Vet J. 2010;3(2):13-17.
  • Zheng WX, Wang F, Cao XL, Pan HY, Liu XY, Hu XM et al. Baicalin protects PC-12 cells from oxidative stress induced by hydrogen peroxide via anti-apoptotic effects. Brain Inj. 2014; 28(2): 227-234. doi:10.3109/02699052.2013.860469.
  • Jang SI, Kim HJ, Hwang KM, Jekal SJ, Pae HO, Choi BM et al. Hepatoprotective effect of baicalin, a major flavone from scutellaria radix, on acetaminophen-ınduced liver injury in mice. Immunopharmacol Immunotoxicol. 2003;25(4):585-594. doi:10.1081/iph-120026443.
  • Su G, Chen G, An X, Wang H, Pei YH. Metabolic profiling analysis of the alleviation effect of treatment with baicalin on cinnabar induced toxicity in rats urine and serum. Front Pharmacol. 2017;17(8):271. doi:10.3389/fphar.2017.00271.

AMELIORATIVE EFFECTS OF BAICALIN AGAINST EXPOSURE TO FLUMETHRIN IN MALE RATS

Year 2024, , 160 - 167, 25.07.2024
https://doi.org/10.34108/eujhs.1389114

Abstract

Flumethrin is a pyrethroid insecticide, while baicalin is a flavonoid with antioxidant, anti-inflammatory, and anticarcinogenic properties. The aim of this study was to investigate the effects of baicalin on biochemical parameters and lipid peroxidation flumethrin-induced in rats. In the study, 42 rats were divided into six groups with each group comprising seven rats. Flumethrin was administrated 15 mg/kg b.w. to second group, flumethrin+baicalin 50 mg/kg b.w. was administrated to third group, flumethrin+baicalin 100 mg/kg b.w. was administrated to fourth group, baicalin 50 mg/kg b.w. was administrated to fifth group, and baicalin 100 mg/kg b.w. was administrated to sixth group. After, blood and tissue samples were collected for biochemical and histopathological evaluations. According to obtained results, when flumethrin-induced group was compared to control, alkaline phosphatase, cholesterol, blood urea nitrogen, uric acid and total protein levels significantly decreased. Also, kidney catalase and plasma glutatione peroxidase, liver catalase and superoxide dismutase activities decreased, but both kidney and liver nitric oxide and melondialdehyde levels increased in flumethrin-induced group. Flumethrin caused to histopathological alterations in tissues. On the other hand, statistically, kidney catalase and plasma glutatione peroxidase, liver catalase and superoxide dismutase activities increased, but nitric oxide and melondialdehyde levels decreased in all groups given baicalin. In additionally, baicalin affected to some biochemical parameters (p<0.05) and regressed to tissue damage. The obtained biochemical results were consistent with histopathological results. In conclusion, this study suggests that baicalin can ameliorate oxidative stress and tissue damage in flumethrin-induced subacute toxication in rats.

Ethical Statement

The this study was carried out with the ethics committee approval dated 09.10.2019 and numbered 19/171 given by the Erciyes University, Animal Experiments Local Ethics Committee.

Supporting Institution

The present study was supported by the Scientific Research Council of Erciyes University, Kayseri, Türkiye

Project Number

TYL-2020-9947

Thanks

The authors would like to thank Erciyes University for financial support and Assoc Prof. Dr. Mehmet Önder Karayiğit for the histopathological examination.

References

  • Gajendiran A, Abraham J. An overview of pyrethroidinsecticides. Front Biol. 2018;13(2):79-90.doi:10.1007/s11515-018-1489-z.
  • FAO/WHO report. Pesticideresidues in food-1996. Evaluations. Part I-Residues, Part II-Toxicology. FAO Plant Production and Protection Paper, 140. Roma: Italy. 1996.251-288. https://www.fao.org/publications/card/en/c/c373c3e9-9195-5b59-95b4-36fbe703868f/. AccessedSeptember 10, 2022.
  • EMEA/MRL/469-98, Summary report, committee for veterinary medicinal products, flumethrin. The European Agency for the Evaluation of Medicinal Products. London; United Kingdom.1998.1-7. https://www.ema.europa.eu/en/documents/mrl-report/flumethrin-summary-report-1-committee-veterinary-medicinal-products_en.pdf.AccessedSeptember 10, 2022.
  • Pisoschi AM, Pop A. The role of antioxidants in the chemistry of oxidative stress: A review. Eur J Med Chem. 2015;97(5):55-74. doi:10.1016/j.ejmech.2015.04.040.
  • Karabulut H, Gülay MŞ. Antioksidanlar. MAEU Vet Fak Derg. 2016;1(1):65-76.doi:10.24880/maeuvfd.260790.
  • Jabłońska-Trypuć A. Pesticides as inducers of oxidative stress. Cell Med Press. 2017;3(8):96-110. doi:10.20455/ros.2017.823.
  • Kanbur M, Eraslan G, Soyer SZ, Altınordulu Ş. The effect of Saw palmetto on flumethrin-induced lipid peroxidation in rats. Pestic Biochem Physiol. 2010;97(1):43-46. doi:10.1016/j.pestbp.2009.11.011.
  • Mishra A, Dewangan G, Mahajan V, Mandal TK. Effect of flumethrin on tissue biochemistry following oral administration in wistar albino rats. Int J Pharma Bio Sci. 2012;3(2):191-200.
  • Singh AK, Singh PK, Dey A, Mishra A, Dewangan G, Chakraborty K et al. Effect of flumethrin on hematological and biochemical changes in rats. Explor Anim Med Res. 2012;1(2):131-136.
  • Salama AM, Talkhan OFA, Khattab MS, Fakhry FM. Protective effect of quercetin against oxidative stress, immuno histochemical and histopathological changes induced by flumethrin. Anim HealthRes J. 2019;22(7):191-200.
  • Robards K, Antolovich M. Analytical chemistry of fruit bioflavonoids: a review. The Analyst. 1997;122(2):122-130.doi:10.1039/A606499J
  • Shieh DE, Liu LT, Lin CC. Antioxidant and free radical scavenging effects of baicalein, baicalin and wogonin. Anticancer Res. 2000;20(5A):2861-2865.
  • Chen YC, Chow JM, Lin CW, Wu CY, Shen SC. Baicalein inhibition of oxidative-stress-induced apoptosis via modulation of ERKs activation and induction of HO-1 gene expression in rat glioma cells C6. Toxicol Appl Pharmacol. 2006;16(2):263-273.doi:10.1016/j.taap.2006.05.008.
  • Tu XK, Yang WZ, Shi SS, Wang CH, Chem CM. Neuroprotective effect of baicalin in a rat model of permanent focal cerebral ischemia. Neurochem Res. 2009;34(9):1626-1634.doi:10.1007/s11064-009-9953-4.
  • Yin F, Liu J, Ji X, Wang Y, Zidichouski J, Zhang J. Baicalin prevents the production of hydrogen peroxide and oxidative stress induced by Ab aggregation in SH-SY5Y cells. Neurosci Lett. 2011;492(2):76-79.doi:10.1016/j.neulet.2011.01.055.
  • Hou J, Wang J, Zhang P et al. Baicalin attenuates proinflammatory cytokine production in oxygen-glucose deprived challenged rat microglial cells by inhibiting TLR4 signaling pathway. Int Immunopharmacol. 2012;14(4):749-757.doi:10.1016/j.intimp.2012.10.013.
  • Wang HZ, Wang HH, Huang SS et al. Inhibitory effect of baicalin on collagen-induced arthritis in rats through the nuclear factor-κB pathway. J Pharmacol Exp Ther.2014;350(2):435-443.doi:10.1124/jpet.114.215145.
  • Zhang Q, Sun J, Wang Y et al. Antimycobacterial and anti-inflammatory mechanisms of baicalin via induced autophagy in macrophages Infected with Mycobacterium tuberculosis. Front Microbiol. 2017;8(2):2142.doi:10.3389/fmicb.2017.02142
  • Noh K, Kang Y, Nepal MR, Jeong KS, Oh DG, Kang MJ, Lee S et al. Role of intestinal microbiota in baicalin-induced drug interaction and it's pharmaco kinetics. Molecules. 2016;21(3):337. doi:10.3390/molecules21030337.
  • Xing J, Chen X, Zhong D. Absorption and enterohepatic circulation of baicalin in rats. Life Sci. 2005;78(2):140-146. doi:10.1016/j.lfs.2005.04.072.
  • Kalapos-Kovács B, Magda B, Jani M, Fekete Z, Szabó PT, Antal I et al. Multiple ABC transporters efflux baicalin. Phytother Res. 2015;29(12):1987-1990. doi:10.1002/ptr.5477.
  • Akao T, Kawabata K, Yanagisawa E, Ishihara K, Mizuhara Y, Wakui Y et al. Balicalin, the predominant flavoneglucuronide of scutellariaeradix, is absorbed from the rat gastro intestinal tract as the agly cone and restored to its original form. J PharmPharm. 2000;52(12):1563-1568. doi:10.1211/0022357001777621.
  • Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin Phenol Reagent. J Biol Chem. 1951;193(1):265-275. doi:10.1016/S0021-9258(19)52451-6
  • Miller GL. Protein determination of large numbers of samples. Anal Chem. 1959;31(5):964. doi:10.1021/ac60149a611.
  • Yoshioka T, Kawada K, Shimada T, Mori M. Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. Am J Obstet Gynecol. 1979;135(3):372-376.doi:10.1016/0002-9378(79)90708-7.
  • Sun Y, Larry W, Oberley R. A simple method for clinical assay of superoxide dismutase. Clin Chem. 1988;34(3):497-500.
  • Tracey WR, Tse J, Carter G. Lipopolysaccharide-induced changes in plasma nitrite and nitrate concentrations in rats and mice: pharmacological evaluation of nitric oxide synthase inhibitors. J Pharmacol Exp Ther. 1995;272(3):1011-1015.
  • Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 1967;70(1):158-169.
  • Luck H. Catalase. IN: Methods of enzymatic analysis. (Bergmeyer HU. Ed.) New York: Academic Press; 1971;3(7A):885-893.
  • Küçükkurt İ, İnce S, Aytekin İ, Birdane YO. The effects of flumethrin and flumethrin+vitamin C application on oxidative stress biomakers in chios sheep. Kocatepe Vet J. 2010;3(2):13-17.
  • Zheng WX, Wang F, Cao XL, Pan HY, Liu XY, Hu XM et al. Baicalin protects PC-12 cells from oxidative stress induced by hydrogen peroxide via anti-apoptotic effects. Brain Inj. 2014; 28(2): 227-234. doi:10.3109/02699052.2013.860469.
  • Jang SI, Kim HJ, Hwang KM, Jekal SJ, Pae HO, Choi BM et al. Hepatoprotective effect of baicalin, a major flavone from scutellaria radix, on acetaminophen-ınduced liver injury in mice. Immunopharmacol Immunotoxicol. 2003;25(4):585-594. doi:10.1081/iph-120026443.
  • Su G, Chen G, An X, Wang H, Pei YH. Metabolic profiling analysis of the alleviation effect of treatment with baicalin on cinnabar induced toxicity in rats urine and serum. Front Pharmacol. 2017;17(8):271. doi:10.3389/fphar.2017.00271.
There are 33 citations in total.

Details

Primary Language English
Subjects Veterinary Pharmacology
Journal Section Research Article
Authors

Esra Nur Üvenç 0000-0002-5682-2789

Feride Koç 0000-0002-3963-5199

Project Number TYL-2020-9947
Early Pub Date July 22, 2024
Publication Date July 25, 2024
Submission Date November 10, 2023
Acceptance Date March 15, 2024
Published in Issue Year 2024

Cite

APA Üvenç, E. N., & Koç, F. (2024). AMELIORATIVE EFFECTS OF BAICALIN AGAINST EXPOSURE TO FLUMETHRIN IN MALE RATS. Sağlık Bilimleri Dergisi, 33(2), 160-167. https://doi.org/10.34108/eujhs.1389114
AMA Üvenç EN, Koç F. AMELIORATIVE EFFECTS OF BAICALIN AGAINST EXPOSURE TO FLUMETHRIN IN MALE RATS. JHS. July 2024;33(2):160-167. doi:10.34108/eujhs.1389114
Chicago Üvenç, Esra Nur, and Feride Koç. “AMELIORATIVE EFFECTS OF BAICALIN AGAINST EXPOSURE TO FLUMETHRIN IN MALE RATS”. Sağlık Bilimleri Dergisi 33, no. 2 (July 2024): 160-67. https://doi.org/10.34108/eujhs.1389114.
EndNote Üvenç EN, Koç F (July 1, 2024) AMELIORATIVE EFFECTS OF BAICALIN AGAINST EXPOSURE TO FLUMETHRIN IN MALE RATS. Sağlık Bilimleri Dergisi 33 2 160–167.
IEEE E. N. Üvenç and F. Koç, “AMELIORATIVE EFFECTS OF BAICALIN AGAINST EXPOSURE TO FLUMETHRIN IN MALE RATS”, JHS, vol. 33, no. 2, pp. 160–167, 2024, doi: 10.34108/eujhs.1389114.
ISNAD Üvenç, Esra Nur - Koç, Feride. “AMELIORATIVE EFFECTS OF BAICALIN AGAINST EXPOSURE TO FLUMETHRIN IN MALE RATS”. Sağlık Bilimleri Dergisi 33/2 (July 2024), 160-167. https://doi.org/10.34108/eujhs.1389114.
JAMA Üvenç EN, Koç F. AMELIORATIVE EFFECTS OF BAICALIN AGAINST EXPOSURE TO FLUMETHRIN IN MALE RATS. JHS. 2024;33:160–167.
MLA Üvenç, Esra Nur and Feride Koç. “AMELIORATIVE EFFECTS OF BAICALIN AGAINST EXPOSURE TO FLUMETHRIN IN MALE RATS”. Sağlık Bilimleri Dergisi, vol. 33, no. 2, 2024, pp. 160-7, doi:10.34108/eujhs.1389114.
Vancouver Üvenç EN, Koç F. AMELIORATIVE EFFECTS OF BAICALIN AGAINST EXPOSURE TO FLUMETHRIN IN MALE RATS. JHS. 2024;33(2):160-7.