AMELIORATIVE EFFECTS OF BAICALIN AGAINST EXPOSURE TO FLUMETHRIN IN MALE RATS

Öz

Flumethrin is a pyrethroid insecticide, while baicalin is a flavonoid with antioxidant, anti-inflammatory, and anticarcinogenic properties. The aim of this study was to investigate the effects of baicalin on biochemical parameters and lipid peroxidation flumethrin-induced in rats. In the study, 42 rats were divided into six groups with each group comprising seven rats. Flumethrin was administrated 15 mg/kg b.w. to second group, flumethrin+baicalin 50 mg/kg b.w. was administrated to third group, flumethrin+baicalin 100 mg/kg b.w. was administrated to fourth group, baicalin 50 mg/kg b.w. was administrated to fifth group, and baicalin 100 mg/kg b.w. was administrated to sixth group. After, blood and tissue samples were collected for biochemical and histopathological evaluations. According to obtained results, when flumethrin-induced group was compared to control, alkaline phosphatase, cholesterol, blood urea nitrogen, uric acid and total protein levels significantly decreased. Also, kidney catalase and plasma glutatione peroxidase, liver catalase and superoxide dismutase activities decreased, but both kidney and liver nitric oxide and melondialdehyde levels increased in flumethrin-induced group. Flumethrin caused to histopathological alterations in tissues. On the other hand, statistically, kidney catalase and plasma glutatione peroxidase, liver catalase and superoxide dismutase activities increased, but nitric oxide and melondialdehyde levels decreased in all groups given baicalin. In additionally, baicalin affected to some biochemical parameters (p<0.05) and regressed to tissue damage. The obtained biochemical results were consistent with histopathological results. In conclusion, this study suggests that baicalin can ameliorate oxidative stress and tissue damage in flumethrin-induced subacute toxication in rats.

Anahtar Kelimeler

• Antioxidant
• Baicalin
• Flumethrin
• Lipid peroxidation
• Toxicity

ERKEK RATLARDA FLUMETRİN MARUZİYETİNE KARŞI BAİKALİNİN İYİLEŞTİRİCİ ETKİLERİ

Öz

Baikalin antioksidan, antiinflamatuar ve antikarsinojenik özelliklere sahip bir flavonoidken, flumetrin bir piretroid insektisittir. Bu çalışmanın amacı, flumetrin ile indüklenmiş ratlarda baikalinin biyokimyasal parametrelere ve lipid peroksidasyonuna etkilerini araştırmaktı. Çalışmada 42 adet rat, her grupta yedi adet rat olacak şekilde altı gruba ayrıldı. Flumetrin ve baikalin mısır yağı içinde tek doz oral gavaj yoluyla 28 gün boyunca verildi: Kontrol grubuna mısır yağı uygulandı. Flumetrin 15 mg/kg c.a. ikinci gruba, flumetrin+baikalin 50 mg/kg c.a. üçüncü gruba, flumetrin+baikalin 100 mg/kg c.a. dördüncü gruba, baikalin 50 mg/kg c.a. beşinci gruba, baikalin 100 mg/kg c.a. altıncı gruba verildi. Daha sonra kan ve doku örnekleri biyokimyasal ve histopatolojik değerlendirmeler için alındı. Elde edilen sonuçlara göre, flumetrin verilen grup kontrol ile karşılaştırıldığında kolesterol, alkalen fosfataz, total protein, üre ve ürik asit düzeyleri anlamlı seviyede azaldı. Ayrıca flumetrin alan grupta böbrek süperoksit dismutaz ve glutatyon peroksidaz ile karaciğer süperoksit dismutaz ve katalaz aktiviteleri azaldı, ancak hem karaciğer hem de böbrek melondialdehit ve nitrik oksit seviyeleri arttı. Flumetrin dokularda histopatolojik değişikliklere neden olmuştur. Öte yandan, baikalin verilen tüm gruplarda istatistiksel olarak böbrek süperoksit dismutaz ve glutatyon peroksidaz ile karaciğer süperoksit dismutaz ve katalaz aktiviteleri arttı, ancak nitrik oksit ve melondialdehit düzeyleri azaldı. Ayrıca baikalin bazı biyokimyasal parametreleri etkiledi (p<0.05) ve doku hasarını azalttı. Elde edilen biyokimyasal sonuçlar histopatolojik sonuçlarla uyumludur. Sonuç olarak, ratlarda flumetrin ile oluşturulan subakut toksikasyonda, baikalin oksidatif stresi ve doku hasarını iyileştirebilir.

Anahtar Kelimeler

• Antioksidan
• Baikalin
• Flumetrin
• Lipid peroksidasyonu
• Toksisite

Kaynakça

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