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EFFECTS OF CHRONIC LEAD INTAKE VIA DRINKING WATER ON SOME HEMATOLOGIC PARAMETERS IN MICE

Year 2004, Volume: 13 Issue: 3, 59 - 63, 01.12.2004

Abstract

The aim of this study was to investigate the
effects of chronic lead intake by drinking water at the
dosages of 250 and 1000 ppm of lead (as lead
acetate) on some hematologic parameter in albino
mice. A total of 120 six-month-old 120 white male
albino mice, weighing 35- 40 g fed with same pellet
feed and water were used in this study. Animals were
equally divided into three groups of 40 animals each.
Group I served as a control. Group II and group III
received 250 ppm and 1000 ppm lead acetate via
drinking water during 120 days, respectively. Blood
samples were taken by cardiac puncture on days 60,
90 and 120 of study from animals. On days 60 and 90
there were no statistical difference in hematological
parameters between groups. On day 120, mice in
group III received 1000 ppm lead ecetate had
significantly lower erythrocyte count, hematocrit and
hemoglobin, MCV and MCH values than the control
mice. Additionally, platelet counts, total leukocyte
counts and lymphocyte ratios were significantly lower
in lead-treated mice than the control animals. On the
other hand, neutrophil ratio in lead treated mice were
significantly elevated compared with the control mice.
In conclusion, it was observed that lead acetate at the
dose of 250 ppm does not affect the hematologic
parameters while lead acetate at the dose of 1000
ppm leads to hematological disorders.

References

  • 1. Rampley CG, Ogden KL. Preliminary studies for removal of lead from surrogate and real soils using a water soluble chelator. Environ Sci Tech 1998, 32: 987-993.
  • 2. Palacios H, Iribarren I, Olalla MJ, Cala V. Lead poisoning of horses in the vicinity of a battery recycling plant. Sci Total Environ 2002, 290: 81-89.
  • 3. Harkness JE, Wagner JE. The biology and medicine of rabbits and rodents. Fourth edition, Williams & Wilkins, Baltimore 1995, pp: 93.
  • 4. Jacob B, Ritz B, Heinrich J, Hoelscher B, Wichman E. The effect of low-l level blood lead on hematologic parameters in children. Environ. Res Sec A 2000, 82:150-159.
  • 5. Othman AI, Sharawy AI, Missry MA. Role of melatonin in ameliorating lead induced haematotoxicity. Phar Res 2004, 50: 301-307.
  • 6. Liu ZP. Lead poisoning combined with cadmium in sheep and horses in the vicinity of non-ferrous metal smelters. Sci Environ 2003, 309: 117-126.
  • 7. Harada K, Miura H. Heme synthesis and iron turnover in rabbits with experimental lead poisoning. Sangyo Igaku 1983, 25: 161-174.
  • 8. Altınsaat Ç, Uzun M, Sulu N, Öztürkmen A. Kobaylarda kurşun asetat uygulamasının bazı hematologic değerler üzerine etkisi. Ankara Üniv Vet Fak Derg 1997, 44:249-258.
  • 9. Moore M R. Hematological effects of lead. Sci Environ 1988, 71: 419-431.
  • 10. Donaldson WE, Knowles SO. Is lead toxicosis a reflection of altered fatty acis composition of membranes Comp Biochem Physiol C 1993, 104: 377-379.
  • 11. De Silva PE. Determination of lead in plasma and studies on its relationship to lead in erythrocytes. Br J Ind Med 1981, 38: 209-217.
  • 12. Rice-Evans. Iron-mediated oxidative stress and erythrocytes. In: J R Harris( Ed.) Blood cell biochemistry (Vol. I), Plenum Press, New York, 1990 pp 429- 453.
  • 13. Levander OA, Morris VC, Ferretti RJ. Filterability of erythrocytes from vitamin Edeficient lead-poisoned rats. J Nutr 1977, 107: 363-372.
  • 14. Yoshida K, Sakurai H, Toyama T. Immunological parameters in lead workers. Sangyo Igaku 1980, 22: 488-93.
  • 15. Van Den Heuvel RL, Leppens H, Schoeters GE. Lead and catechol hematoxicity in vitro using human and murine hematopoietic progenitor cells. Cell Biology and Toxicology 1999, 15: 101-110.
  • 16. Peng S, Zhang C, Wang C, et al. The investigation on the changes of hematological parameters in the occupationally lead exposed workers. Zhonghau Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2002, 20: 334.

Farelerde İçme Suyu ile Kronik Kurşun Alımının Bazı Hematolojik Parametreler Üzerine Etkisi

Year 2004, Volume: 13 Issue: 3, 59 - 63, 01.12.2004

Abstract

Bu çalışma, 250 ve 1000 ppm dozunda kurşun asetat içeren içme sularının farelere kronik uygulanmasının bazı hematolojik parametrelerelere etkisini incelemek amacıyla yapılmıştır. Çalışmada 6 aylık, 35-40 g ağırlığında 120 erkek beyaz fare kullanıldı. Hayvanlar her bir grupta 40 fare olacak şekilde üç eşit gruba ayrıldı. I.grup control grubu olarak tutulurken II. ve III. gruptaki farelere 250 ve 1000 ppm kurşun asetat içeren su 120 gün süreyle verildi. Hayvanlardan 60., 90. ve 120. günlerde kalpten kan örnekleri alınarak incelendi. İncelenen hematolojik parametrelerde 60. ve 90. günlerde her hangi bir farklılık belirlenemedi. 1000 ppm dozda kurşun alan III. gruptaki hayvanların kontrol gurubuna göre eritrosit, lökosit, platelet sayısı ve lenfosit oranı ile ile hemoglobin, hematokrit, MCV ve MCH değerlerinde önemli bir azalma gözlenirken nötrofil oranında ise önemli bir artma tespit edilmiştir. Sonuç olarak, 250 ppm dozda kronik olarak kurşun alımının hematalojik parametreleri etkilemediği buna karşılık 1000 ppm dozda kurşun asetatın alımının ise hematolojik bozukluklara yol açtığı gözlenmiştir

References

  • 1. Rampley CG, Ogden KL. Preliminary studies for removal of lead from surrogate and real soils using a water soluble chelator. Environ Sci Tech 1998, 32: 987-993.
  • 2. Palacios H, Iribarren I, Olalla MJ, Cala V. Lead poisoning of horses in the vicinity of a battery recycling plant. Sci Total Environ 2002, 290: 81-89.
  • 3. Harkness JE, Wagner JE. The biology and medicine of rabbits and rodents. Fourth edition, Williams & Wilkins, Baltimore 1995, pp: 93.
  • 4. Jacob B, Ritz B, Heinrich J, Hoelscher B, Wichman E. The effect of low-l level blood lead on hematologic parameters in children. Environ. Res Sec A 2000, 82:150-159.
  • 5. Othman AI, Sharawy AI, Missry MA. Role of melatonin in ameliorating lead induced haematotoxicity. Phar Res 2004, 50: 301-307.
  • 6. Liu ZP. Lead poisoning combined with cadmium in sheep and horses in the vicinity of non-ferrous metal smelters. Sci Environ 2003, 309: 117-126.
  • 7. Harada K, Miura H. Heme synthesis and iron turnover in rabbits with experimental lead poisoning. Sangyo Igaku 1983, 25: 161-174.
  • 8. Altınsaat Ç, Uzun M, Sulu N, Öztürkmen A. Kobaylarda kurşun asetat uygulamasının bazı hematologic değerler üzerine etkisi. Ankara Üniv Vet Fak Derg 1997, 44:249-258.
  • 9. Moore M R. Hematological effects of lead. Sci Environ 1988, 71: 419-431.
  • 10. Donaldson WE, Knowles SO. Is lead toxicosis a reflection of altered fatty acis composition of membranes Comp Biochem Physiol C 1993, 104: 377-379.
  • 11. De Silva PE. Determination of lead in plasma and studies on its relationship to lead in erythrocytes. Br J Ind Med 1981, 38: 209-217.
  • 12. Rice-Evans. Iron-mediated oxidative stress and erythrocytes. In: J R Harris( Ed.) Blood cell biochemistry (Vol. I), Plenum Press, New York, 1990 pp 429- 453.
  • 13. Levander OA, Morris VC, Ferretti RJ. Filterability of erythrocytes from vitamin Edeficient lead-poisoned rats. J Nutr 1977, 107: 363-372.
  • 14. Yoshida K, Sakurai H, Toyama T. Immunological parameters in lead workers. Sangyo Igaku 1980, 22: 488-93.
  • 15. Van Den Heuvel RL, Leppens H, Schoeters GE. Lead and catechol hematoxicity in vitro using human and murine hematopoietic progenitor cells. Cell Biology and Toxicology 1999, 15: 101-110.
  • 16. Peng S, Zhang C, Wang C, et al. The investigation on the changes of hematological parameters in the occupationally lead exposed workers. Zhonghau Lao Dong Wei Sheng Zhi Ye Bing Za Zhi 2002, 20: 334.
There are 16 citations in total.

Details

Other ID JA75UY75UK
Journal Section Research Article
Authors

Nazmi Çetin This is me

Dinç Eşsiz This is me

Gökhan Eraslan This is me

Hakan Saltaş This is me

Publication Date December 1, 2004
Submission Date December 1, 2004
Published in Issue Year 2004 Volume: 13 Issue: 3

Cite

APA Çetin, N., Eşsiz, D., Eraslan, G., Saltaş, H. (2004). Farelerde İçme Suyu ile Kronik Kurşun Alımının Bazı Hematolojik Parametreler Üzerine Etkisi. Sağlık Bilimleri Dergisi, 13(3), 59-63.
AMA Çetin N, Eşsiz D, Eraslan G, Saltaş H. Farelerde İçme Suyu ile Kronik Kurşun Alımının Bazı Hematolojik Parametreler Üzerine Etkisi. JHS. December 2004;13(3):59-63.
Chicago Çetin, Nazmi, Dinç Eşsiz, Gökhan Eraslan, and Hakan Saltaş. “Farelerde İçme Suyu Ile Kronik Kurşun Alımının Bazı Hematolojik Parametreler Üzerine Etkisi”. Sağlık Bilimleri Dergisi 13, no. 3 (December 2004): 59-63.
EndNote Çetin N, Eşsiz D, Eraslan G, Saltaş H (December 1, 2004) Farelerde İçme Suyu ile Kronik Kurşun Alımının Bazı Hematolojik Parametreler Üzerine Etkisi. Sağlık Bilimleri Dergisi 13 3 59–63.
IEEE N. Çetin, D. Eşsiz, G. Eraslan, and H. Saltaş, “Farelerde İçme Suyu ile Kronik Kurşun Alımının Bazı Hematolojik Parametreler Üzerine Etkisi”, JHS, vol. 13, no. 3, pp. 59–63, 2004.
ISNAD Çetin, Nazmi et al. “Farelerde İçme Suyu Ile Kronik Kurşun Alımının Bazı Hematolojik Parametreler Üzerine Etkisi”. Sağlık Bilimleri Dergisi 13/3 (December 2004), 59-63.
JAMA Çetin N, Eşsiz D, Eraslan G, Saltaş H. Farelerde İçme Suyu ile Kronik Kurşun Alımının Bazı Hematolojik Parametreler Üzerine Etkisi. JHS. 2004;13:59–63.
MLA Çetin, Nazmi et al. “Farelerde İçme Suyu Ile Kronik Kurşun Alımının Bazı Hematolojik Parametreler Üzerine Etkisi”. Sağlık Bilimleri Dergisi, vol. 13, no. 3, 2004, pp. 59-63.
Vancouver Çetin N, Eşsiz D, Eraslan G, Saltaş H. Farelerde İçme Suyu ile Kronik Kurşun Alımının Bazı Hematolojik Parametreler Üzerine Etkisi. JHS. 2004;13(3):59-63.