EFFECTS OF CHRONIC LEAD INTAKE VIA DRINKING WATER ON SOME HEMATOLOGIC PARAMETERS IN MICE

Yıl 2004, Cilt: 13 Sayı: 3, 59 - 63, 01.12.2004

Nazmi Çetin Dinç Eşsiz Gökhan Eraslan Hakan Saltaş

Öz

The aim of this study was to investigate the
effects of chronic lead intake by drinking water at the
dosages of 250 and 1000 ppm of lead (as lead
acetate) on some hematologic parameter in albino
mice. A total of 120 six-month-old 120 white male
albino mice, weighing 35- 40 g fed with same pellet
feed and water were used in this study. Animals were
equally divided into three groups of 40 animals each.
Group I served as a control. Group II and group III
received 250 ppm and 1000 ppm lead acetate via
drinking water during 120 days, respectively. Blood
samples were taken by cardiac puncture on days 60,
90 and 120 of study from animals. On days 60 and 90
there were no statistical difference in hematological
parameters between groups. On day 120, mice in
group III received 1000 ppm lead ecetate had
significantly lower erythrocyte count, hematocrit and
hemoglobin, MCV and MCH values than the control
mice. Additionally, platelet counts, total leukocyte
counts and lymphocyte ratios were significantly lower
in lead-treated mice than the control animals. On the
other hand, neutrophil ratio in lead treated mice were
significantly elevated compared with the control mice.
In conclusion, it was observed that lead acetate at the
dose of 250 ppm does not affect the hematologic
parameters while lead acetate at the dose of 1000
ppm leads to hematological disorders.

Anahtar Kelimeler

Mouse, chronic lead intake, hematologic parameter

Farelerde İçme Suyu ile Kronik Kurşun Alımının Bazı Hematolojik Parametreler Üzerine Etkisi

Öz

Bu çalışma, 250 ve 1000 ppm dozunda kurşun asetat içeren içme sularının farelere kronik uygulanmasının bazı hematolojik parametrelerelere etkisini incelemek amacıyla yapılmıştır. Çalışmada 6 aylık, 35-40 g ağırlığında 120 erkek beyaz fare kullanıldı. Hayvanlar her bir grupta 40 fare olacak şekilde üç eşit gruba ayrıldı. I.grup control grubu olarak tutulurken II. ve III. gruptaki farelere 250 ve 1000 ppm kurşun asetat içeren su 120 gün süreyle verildi. Hayvanlardan 60., 90. ve 120. günlerde kalpten kan örnekleri alınarak incelendi. İncelenen hematolojik parametrelerde 60. ve 90. günlerde her hangi bir farklılık belirlenemedi. 1000 ppm dozda kurşun alan III. gruptaki hayvanların kontrol gurubuna göre eritrosit, lökosit, platelet sayısı ve lenfosit oranı ile ile hemoglobin, hematokrit, MCV ve MCH değerlerinde önemli bir azalma gözlenirken nötrofil oranında ise önemli bir artma tespit edilmiştir. Sonuç olarak, 250 ppm dozda kronik olarak kurşun alımının hematalojik parametreleri etkilemediği buna karşılık 1000 ppm dozda kurşun asetatın alımının ise hematolojik bozukluklara yol açtığı gözlenmiştir

Anahtar Kelimeler

Fare, kronik kurşun alımı, hematolojik parametre

Kaynakça

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