BUZAĞI KOKSİDİOZİSİNDE LİPİD PEROKSİDASYON DÜZEYİ VE ANTİOKSİDAN ENZİM AKTİVİTELERİ

Year 2008, Volume: 17 Issue: 3, 131 - 136, 01.12.2008

Adem Tufan Yücel Çam

Abstract

Bu çalışmada, buzağı koksidiozisinde plazma
malondialdehit (MDA) düzeyi ile eritrosit süperoksit
dismutaz (SOD), katalaz (CAT) ve glutasyon
peroksidaz (GSH-Px) enzim aktivitelerinin
değerlendirilmesi amaçlandı. Çalışmada, yaşları 2
hafta ile 6 ay arasında değişen toplam 30 adet buzağı
kullanıldı. Bu hayvanların 15’i sağlıklı buzağı
grubunu oluştururken, 15’i koksidiozisli buzağı
grubunu oluşturdu. Hayvanların klinik muayenesi
yapıldı. Parazitolojik muayene için buzağılardan
dışkı numuneleri toplandı. Koksidiozisli buzağıların
tümünün dışkı numunelerinde Eimeria spp. oosistleri
gözlemlendi. Plazma MDA düzeyi ile eritrosit GSHPx, CAT ve SOD aktivitelerinin belirlenmesi için
buzağılardan kan numuneleri toplandı. Kontrolle
karşılaştırıldığında koksidiozisli buzağılarda plazma
MDA düzeyinin önemli derecede daha yüksek olduğu
belirlendi (p<0,05). Buna karşın, koksidiozisli
buzağılarda eritrosit SOD, CAT ve GSH-Px
aktiviteleri kontrol grubuna göre istatistiksel açıdan
önemli oranda daha düşüktü (p<0,05).
Sonuç olarak, hasta buzağılarda plazma MDA
düzeyinde artış, eritrosit CAT, SOD ve GSH-Px
aktivitelerinde düşme koksidiozisin lipid
peroksidasyonuna yol açtığını gösterebilir. Bu durum
koksidiozisli buzağılarda oksidan ve antioksidan
arasındaki dengenin oksidan lehine bozulduğunu
göstermektedir.

Keywords

Antioksidan enzim, buzağı, Eimeria spp., koksidiozis, lipid peroksidasyon

Lipid Peroxidation Level and Antioxidant Enzyme Activities in Calves Coccidiosis

Abstract

This study was performed to evaluate the
plasma malondialdehyde (MDA) level, and
erythrocyte glutation peroxidase (GSH-Px), catalase
(CAT) and superoxide dismutase (SOD) activities in
calves with coccidiosis. In this study, 30 calves, aged
between 2 weeks to 6 months were used. Fifteen of
these animal were included in the healthy calves
group, while the other 15 consisted of calves group
with coccidiosis. Clinical examinations of the
animals were carried out. Faecel samples for
parasitological examination were collected from
calves. Eimeria spp. oocysts were seen in the faecal
samples of the all calves with coccidiosis. Blood
samples from the calves were collected for
determination of plasma MDA level, and erythrocyte
GSH-Px, CAT and SOD activities. Plasma MDA
level was significantly higher in the young cattle with
ringworm compared to controls (p<0.05). However,
the erythrocyte SOD, CAT and GSH-Px activities in
the calves with coccidiosis was significantly lower
(p<0.05) than that of the controls.
In conclusion, the determination of increasing in
plasma MDA levels and decreasing in erythrocytes
CAT, SOD and GSH-Px activities in the infected
calves suggested that coccidiosis caused lipid
peroxidation. This indicated that the equilibrium
between oxidants and antioxidants impaired in
favour of oxidants in the calves with coccidiosis.

Keywords

Antioxidant enzyme, calves, Eimeria spp., coccidiosis, Lipid peroxidation

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