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DONÖR İNEKLERİN BESLEMESİNDE KORUNMUŞ METİYONİN KULLANILMASININ EMBRİYO KALİTESİ ÜZERİNE ETKİSİNİN ARAŞTIRILMASI

Year 2017, Volume: 26 Issue: 2, 112 - 118, 01.07.2017

Abstract

Bu çalışmanın amacı, donör ineklerin beslenmesinde rumende
korunmuş metiyonin katkısının embriyo kalitesine etkisini
araştırmaktır. Çalışmada 14 adet Holştayn ırkı inek kontrol ve
deneme grubu olarak ikiye ayrıldı. Donör olarak kullanılan
ineklerin canlı ağırlık (500-550 kg), laktasyon dönemi (1-2
laktasyon) ve süt verimi (günlük 30-35 litre) gibi özellikleri
benzerdir. Çalışmada alıcı olarak ise 56 tane Holştayn inek
kullanıldı (ortalama 550 kg canlı ağırlığında ve 1. laktasyonda).
Hayvanlar mısır silajı, yonca kuru otu, saman ve konsantre
yemden oluşan bazal rasyon ile beslendi. Deneme grubunda
bulunan ineklere deneme süresince kontrol rasyonuna ilave
olarak her hayvan için 15 gram/gün korunmuş metiyonin oral
yolla verildi. Kontrol ve deneme grubu 20 gün süreyle yukarıda
bahsedilen rasyon ile beslendikten sonra “Süperovulasyon
Protokolü” uygulamalarına başlandı. Süperovulasyon Protokolü uygulamaları esnasında besleme programında değişiklik
yapılmadı. FSH hormonu enjeksiyonu yapılmadan ve uterusu
yıkamaya başlamadan önce korpus luteum (Cl) sayısı, yoğunluğu ve çapı ultrason yardımı ile belirlendi. Araştırmanın sonucunda, süperovulasyon sonrası Cl sayısı bakımından gruplar
arasında bir farklılığın olmadığı tespit edildi (P>0.05). Embriyolar uterus yıkaması yapılarak toplandı. Bulunan embriyolar
kalite ve gelişim safhalarına göre sınıflandırıldı. Gruplar arasında bir farklılık görülmedi (P>0.05). Bulunan embriyolar taşıyıcı
hayvanlara transfer edildi. Embriyo tranferinden sonra 50. gün
de gebelik kontrolleri ultrason ile yapıldı. Gebelik oranı, kontrol ve deneme grubu arasında herhangi bir farklılık göstermedi
(P>0.05).
Sonuç olarak, korunmuş metiyoninin toplam embriyo, transfer
edilebilen ve edilemeyen embriyo, gebelik ve fertilize olmamış
ovum sayıları ve oranları arasında istatistiki bakımından etkisi
önemli bulunmadı.

References

  • 1. Smith C. Applications of embryo transfer in animal breeding. Theriogenology 1988; 29(1):203-212.
  • 2. Santos JEP, Cerri RLA, Sartori R. Nutritional management of the donor cow. Theriogenology 2008; 69(1):88–97.
  • 3. Ayaşan T, Karakozak E. Donör ineklerin beslenmesi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2010; 16(3):523-530.
  • 4. Robinson JJ, Ashworth CJ, Rooke JA, Mitchell LM, McEvoy TG. Nutrition and fertility in ruminant livestock. Animal Feed Science and Technology 2006; 126(3):259-276.
  • 5. Ferguson JD, Chalupa W. Impact of protein nutrition on reproduction in dairy cows. Journal of Dairy Science 1989; 72(3):746-766.
  • 6. Butler WR. Effect of protein nutrition on ovarian and uterine physiology in dairy cattle. Journal of Dairy Science 1998; 81(9):2533-2539.
  • 7. Nikkah A, Kianzad D, Hajhosseini A, Zaybeyk A. Protected methionıne prolonged provision summer production and reproduction of lactating dairy cows. Pakistan Journal of Biological Sciences 16(12):558-563, 2013 ISSN 1028-880 / DOI:10.3923/pjbs. 2013.558.563.
  • 8. Pen˜’agaricano F, Souza AH, Carvalho PD, et al. Effect of maternal methionine supplementation on the transcriptome of bovine preimplantation embryos. PloS one 2013; 8(8): e72302.
  • 9. Coelho CN, Klein NW. Methionine and neural tube closure in cultured rat embryos: morphological and biochemical analyses. Teratology 1990; 42 (4):437-451.
  • 10. Ikeda S, Sugimoto M, Kume S. Importance of methionine metabolism in morula- to-blastocyst transition in bovine preimplantation embryos. Journal of Reproductive and Development 2012; 58(1):91-97.
  • 11. Bonılla L, Luchını D, Devıllard E, Hansen PJ. Methionine Requirements for the Preimplantation Bovine Embryo Journal of Reproduction and Development 2010; 56(5):527-532.
  • 12. Rulquın H, Pısulewskı PM, Verıte R, Guınard J. Milk production and composition as a function of postruminal lysine and methionine supply: a nutrient-response approach. Livestock Production Science 1993; 37:69-90.
  • 13. Alonso M, Maquivar M, Galina CS, et al. Effect of ruminally protected methionine on the productive and reproductive performance of grazing Bos indicus heifers raised in the humid tropics of Costa Rica. Tropical Animal Health And Production 2008; 40(8):667-672.
  • 14. Gök K. Türkiyede Embriyo Transferinin Önemi-2. Tarım ve Teknik Dergisi Kayseri 2015; 2(4):56-59.
  • 15. AOAC. Official Methods of Analysis of the Association of Official Analytical Chemists. 14th ed., Inc., Arlington, Virginia 1984.
  • 16. Van Soest PJ, Robertson JB, Lewis BA. Symposium: carbohydrate methodology, metabolism, and nutritional implications in dairy cattle. Journal of Dairy Science 1991; 74(10):3583-3597.
  • 17. Minitab Release 14, Statistical Software, Minitab Inc,USA2004.
  • 18. Wiltbank MC. Garcia-Guerra A, Carvalho PD, et al. Effects of energy and protein nutrition in the dam on embryonic development. Animal Reproduction 2014; 11(3):168-182.
  • 19. İzmirli M, Tufan T, Alptekin D. DNA metilasyonu. Arşiv Kaynak Tarama Dergisi 2012; 21(4):274- 282.
  • 20. Dikmen M. Metilentetrahidrofolat Redüktaz (MTHFR) enziminin moleküler biyolojisi ve hastalıklarla ilişkisi. Kocatepe Tıp Dergisi 2004; 5( 2):9- 16.
  • 21. Souza AH, Carvalho PD, Dresch AR, et al. Effect of methionine supplementation during postpartum period in dairy cows II: embryo quality. Theriogenology 2016; 85(9):1669-1679.
  • 22. Hızlı H, Ayaşan T, Kılıçalp N, ve ark. Verici inek ve düvelerde tekrarlı superovulasyonların embriyo kalitesi üzerine etkisi. YYÜ Veteriner Fakültesi Dergisi 2012; 23(1):11-14.
  • 23. Ayaşan T, Hızlı H, Çamlıdağ A, Kara U, Gök K, ve ark. The determination of relationship between milk production and the quality of embryo of donör cows. Indian Journal of Animal Sciences 2011; 81(9):912-914.
  • 24. Hussein MM, Aziz RA, Abdel-Wahab A, El-Said H. Preliminary study of factors affecting the superovulatory response of high producing dairy cows super stimulate dregardless of thestage of estrouscycle in Egypt. Beni-Suef University Journal ofBasic and Applied Sciences 2014; 3(4):286-292.
  • 25. Novotny F, Lazar G, Valocky I, et al. Relation ship between milk production in donor cows and the yield and quality of embryos. Bullet Veterinary Institute Pulawy 2005; 49:303–305.
  • 26. Nebel RL, Mc-Gilliard ML. Interactions of high milk yield and reproductive performance in dairy cows. Journal of Dairy Science 1993; 76(10):3257– 3268.
  • 27. Acosta DAV, Denicol AC, Skenendore C, et al. Effect of methionine supplementation on methylation and lipid accumulation of the preimplantation embryo in dairy cows. Journal of Dairy Science 2014; 97:9-25.
  • 28. Toledo MZ, Baez GM, Garcia-Guerra A, et al. Effect of Supplementation with Rumenprotected Methionine on Reproduction in Lactating Dairy Cows Daıry Scıence Showcase 2015; Nov 3:1-27.

Searching the Effect of Using Protected Metiıonine on Embryo Qality Wiıle Feeding Donor Cows

Year 2017, Volume: 26 Issue: 2, 112 - 118, 01.07.2017

Abstract

The objective of this study was to investigate the effect of
ruminally protected methionine on embryo quality of donor
cows. In this study, a total of 14 Holstein cows were randomly
allocated into two groups, one control and one treatment
group. The properties of donor cows such as average weight
(500-550 kg), lactation period (1-2 lactation), milk production
(between 30-35 kg milk/day). Were similar a total of 56
Holstein cows were used as recipient animals (average 550 kg
live weight, 1st lactation). Feed with a basal ration was
composed of corn silage, alfalfa hay, straw and a concentrate
mix. The duration of the study 15 grams/day protected
methionine was given orally as an addition to a control ration
to the cows in the treatment group. Control and treatment
group were fed through 20 days as it was mentioned before.
Then, ‘Superovulation Protocol’ applications were started.
During the application of Superovulation Protocol, no change
was made in the feeding programme. Before injecting FSH and
washing uterus, the numbers of corpus luteum, density of
corpus luteum and diameter of corpus luteum (CI) were
determined by ultrasonography. As a result of the research,
after the Superovulation, it was seen that there was no
difference between the groups in terms of the Cl numbers
(P>0.05). Embryos were picked up by flushing the uterus.
Found embryos were classified according to their quality and
development phase. There was no statistically significant
difference between the groups (P>0.05). After the embryos
which were found were transferred to the recipient cows,
pregnancy control was performed by ultrasound controls in
50th day. No statistically significant difference was detected in
pregnancy rates between the control and treated group
(P>0.05).
In conclusion, there was not found any statistically significant
difference between groups in terms of the number of total
embryos, number of transferable embryos and not
transferable embryos, preganacy rate and number of
unfertilized ovum

References

  • 1. Smith C. Applications of embryo transfer in animal breeding. Theriogenology 1988; 29(1):203-212.
  • 2. Santos JEP, Cerri RLA, Sartori R. Nutritional management of the donor cow. Theriogenology 2008; 69(1):88–97.
  • 3. Ayaşan T, Karakozak E. Donör ineklerin beslenmesi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 2010; 16(3):523-530.
  • 4. Robinson JJ, Ashworth CJ, Rooke JA, Mitchell LM, McEvoy TG. Nutrition and fertility in ruminant livestock. Animal Feed Science and Technology 2006; 126(3):259-276.
  • 5. Ferguson JD, Chalupa W. Impact of protein nutrition on reproduction in dairy cows. Journal of Dairy Science 1989; 72(3):746-766.
  • 6. Butler WR. Effect of protein nutrition on ovarian and uterine physiology in dairy cattle. Journal of Dairy Science 1998; 81(9):2533-2539.
  • 7. Nikkah A, Kianzad D, Hajhosseini A, Zaybeyk A. Protected methionıne prolonged provision summer production and reproduction of lactating dairy cows. Pakistan Journal of Biological Sciences 16(12):558-563, 2013 ISSN 1028-880 / DOI:10.3923/pjbs. 2013.558.563.
  • 8. Pen˜’agaricano F, Souza AH, Carvalho PD, et al. Effect of maternal methionine supplementation on the transcriptome of bovine preimplantation embryos. PloS one 2013; 8(8): e72302.
  • 9. Coelho CN, Klein NW. Methionine and neural tube closure in cultured rat embryos: morphological and biochemical analyses. Teratology 1990; 42 (4):437-451.
  • 10. Ikeda S, Sugimoto M, Kume S. Importance of methionine metabolism in morula- to-blastocyst transition in bovine preimplantation embryos. Journal of Reproductive and Development 2012; 58(1):91-97.
  • 11. Bonılla L, Luchını D, Devıllard E, Hansen PJ. Methionine Requirements for the Preimplantation Bovine Embryo Journal of Reproduction and Development 2010; 56(5):527-532.
  • 12. Rulquın H, Pısulewskı PM, Verıte R, Guınard J. Milk production and composition as a function of postruminal lysine and methionine supply: a nutrient-response approach. Livestock Production Science 1993; 37:69-90.
  • 13. Alonso M, Maquivar M, Galina CS, et al. Effect of ruminally protected methionine on the productive and reproductive performance of grazing Bos indicus heifers raised in the humid tropics of Costa Rica. Tropical Animal Health And Production 2008; 40(8):667-672.
  • 14. Gök K. Türkiyede Embriyo Transferinin Önemi-2. Tarım ve Teknik Dergisi Kayseri 2015; 2(4):56-59.
  • 15. AOAC. Official Methods of Analysis of the Association of Official Analytical Chemists. 14th ed., Inc., Arlington, Virginia 1984.
  • 16. Van Soest PJ, Robertson JB, Lewis BA. Symposium: carbohydrate methodology, metabolism, and nutritional implications in dairy cattle. Journal of Dairy Science 1991; 74(10):3583-3597.
  • 17. Minitab Release 14, Statistical Software, Minitab Inc,USA2004.
  • 18. Wiltbank MC. Garcia-Guerra A, Carvalho PD, et al. Effects of energy and protein nutrition in the dam on embryonic development. Animal Reproduction 2014; 11(3):168-182.
  • 19. İzmirli M, Tufan T, Alptekin D. DNA metilasyonu. Arşiv Kaynak Tarama Dergisi 2012; 21(4):274- 282.
  • 20. Dikmen M. Metilentetrahidrofolat Redüktaz (MTHFR) enziminin moleküler biyolojisi ve hastalıklarla ilişkisi. Kocatepe Tıp Dergisi 2004; 5( 2):9- 16.
  • 21. Souza AH, Carvalho PD, Dresch AR, et al. Effect of methionine supplementation during postpartum period in dairy cows II: embryo quality. Theriogenology 2016; 85(9):1669-1679.
  • 22. Hızlı H, Ayaşan T, Kılıçalp N, ve ark. Verici inek ve düvelerde tekrarlı superovulasyonların embriyo kalitesi üzerine etkisi. YYÜ Veteriner Fakültesi Dergisi 2012; 23(1):11-14.
  • 23. Ayaşan T, Hızlı H, Çamlıdağ A, Kara U, Gök K, ve ark. The determination of relationship between milk production and the quality of embryo of donör cows. Indian Journal of Animal Sciences 2011; 81(9):912-914.
  • 24. Hussein MM, Aziz RA, Abdel-Wahab A, El-Said H. Preliminary study of factors affecting the superovulatory response of high producing dairy cows super stimulate dregardless of thestage of estrouscycle in Egypt. Beni-Suef University Journal ofBasic and Applied Sciences 2014; 3(4):286-292.
  • 25. Novotny F, Lazar G, Valocky I, et al. Relation ship between milk production in donor cows and the yield and quality of embryos. Bullet Veterinary Institute Pulawy 2005; 49:303–305.
  • 26. Nebel RL, Mc-Gilliard ML. Interactions of high milk yield and reproductive performance in dairy cows. Journal of Dairy Science 1993; 76(10):3257– 3268.
  • 27. Acosta DAV, Denicol AC, Skenendore C, et al. Effect of methionine supplementation on methylation and lipid accumulation of the preimplantation embryo in dairy cows. Journal of Dairy Science 2014; 97:9-25.
  • 28. Toledo MZ, Baez GM, Garcia-Guerra A, et al. Effect of Supplementation with Rumenprotected Methionine on Reproduction in Lactating Dairy Cows Daıry Scıence Showcase 2015; Nov 3:1-27.
There are 28 citations in total.

Details

Other ID JA88RD59TA
Journal Section Research Article
Authors

Kurtuluş Gök This is me

Berrin Kocaoğlu Güçlü This is me

Publication Date July 1, 2017
Submission Date July 1, 2017
Published in Issue Year 2017 Volume: 26 Issue: 2

Cite

APA Gök, K., & Güçlü, B. K. (2017). DONÖR İNEKLERİN BESLEMESİNDE KORUNMUŞ METİYONİN KULLANILMASININ EMBRİYO KALİTESİ ÜZERİNE ETKİSİNİN ARAŞTIRILMASI. Sağlık Bilimleri Dergisi, 26(2), 112-118.
AMA Gök K, Güçlü BK. DONÖR İNEKLERİN BESLEMESİNDE KORUNMUŞ METİYONİN KULLANILMASININ EMBRİYO KALİTESİ ÜZERİNE ETKİSİNİN ARAŞTIRILMASI. JHS. July 2017;26(2):112-118.
Chicago Gök, Kurtuluş, and Berrin Kocaoğlu Güçlü. “DONÖR İNEKLERİN BESLEMESİNDE KORUNMUŞ METİYONİN KULLANILMASININ EMBRİYO KALİTESİ ÜZERİNE ETKİSİNİN ARAŞTIRILMASI”. Sağlık Bilimleri Dergisi 26, no. 2 (July 2017): 112-18.
EndNote Gök K, Güçlü BK (July 1, 2017) DONÖR İNEKLERİN BESLEMESİNDE KORUNMUŞ METİYONİN KULLANILMASININ EMBRİYO KALİTESİ ÜZERİNE ETKİSİNİN ARAŞTIRILMASI. Sağlık Bilimleri Dergisi 26 2 112–118.
IEEE K. Gök and B. K. Güçlü, “DONÖR İNEKLERİN BESLEMESİNDE KORUNMUŞ METİYONİN KULLANILMASININ EMBRİYO KALİTESİ ÜZERİNE ETKİSİNİN ARAŞTIRILMASI”, JHS, vol. 26, no. 2, pp. 112–118, 2017.
ISNAD Gök, Kurtuluş - Güçlü, Berrin Kocaoğlu. “DONÖR İNEKLERİN BESLEMESİNDE KORUNMUŞ METİYONİN KULLANILMASININ EMBRİYO KALİTESİ ÜZERİNE ETKİSİNİN ARAŞTIRILMASI”. Sağlık Bilimleri Dergisi 26/2 (July 2017), 112-118.
JAMA Gök K, Güçlü BK. DONÖR İNEKLERİN BESLEMESİNDE KORUNMUŞ METİYONİN KULLANILMASININ EMBRİYO KALİTESİ ÜZERİNE ETKİSİNİN ARAŞTIRILMASI. JHS. 2017;26:112–118.
MLA Gök, Kurtuluş and Berrin Kocaoğlu Güçlü. “DONÖR İNEKLERİN BESLEMESİNDE KORUNMUŞ METİYONİN KULLANILMASININ EMBRİYO KALİTESİ ÜZERİNE ETKİSİNİN ARAŞTIRILMASI”. Sağlık Bilimleri Dergisi, vol. 26, no. 2, 2017, pp. 112-8.
Vancouver Gök K, Güçlü BK. DONÖR İNEKLERİN BESLEMESİNDE KORUNMUŞ METİYONİN KULLANILMASININ EMBRİYO KALİTESİ ÜZERİNE ETKİSİNİN ARAŞTIRILMASI. JHS. 2017;26(2):112-8.