TİYENİL-TİYAZOL-ARİL-TİYOÜRE HİBRİT MOLEKÜLLERİN SENTEZİ VE BİYOLOJİK AKTİVİTELERİNİN APOPTOTİK YOL ÜZERİNDEN ANTİ-KANSER ETKİLERİNİN ARAŞTIRILMASI

Year 2019, Volume: 28 Issue: 2, 87 - 93, 20.09.2019

Şengül Dilem Doğan Sümeyye Uğur Ayşe Kübra Karaboğa Arslan Ebru Öztürk Ahmet Cumaoğlu Mükerrem Betül Yerer

https://doi.org/10.34108/eujhs.493005

Cited By: 1

Abstract

Tiyenil-tiyazol-aril-tiyoüre
türevleri (4a-4e) amino-4-(2-tiyenil)tiyazol ve fenilizotiyasiyanatların
reaksiyonları sonucu sentezlenmiştir. Sentezlenen bileşikler IR, ¹H-NMR
ve kütle spektrumları ile karekterize edildi. Beş tiyoüre türevinin
anti-proliferatif etkileri A549 (KHDAK) hücrelerinde gerçek zamanlı hücre
analizörü xCELLigence sistemi ile incelendi. Gerçek zamanlı sistemde sitotoksik
olduğu gösterilmiş olan 2 tiyoürenin moleküler etki mekanizmaları,
mitokondriyal membran potansiyeli ΔψM spektrofotometrik olarak, sitokrom c
western blot methoduyla ve Bcl-2 ise qRT-PCR ile araştırılmıştır. 4e
diğerlerinden daha etkili bulunmuştur. 4e
IC₅₀ değerleri 24 ve 48 saat için sırasıyla 29,6 µM ve 22,3 µM
olarak hesaplanmıştır. Bir triflorometil (-CF₃) grubunu içeren 4e
molekülü bir anti-kanserojen madde olarak düşünülebilir ve daha ileri
araştırmalar yapılabilir.0000-0003-1761-188X
 

Keywords

Tiyoüre, Antikanser activite, Tiyazol, Sentez

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