Bir kobay (Cavia porcellus) kolonisinde akut nitrat-nitrit zehirlenmesi

Year 2013, Volume: 29 Issue: 2, 106 - 109, 01.06.2013

Nakulan Valsala Rajesh Veluchamy Kumar Palanisamy Ganapathi Marudhai Thangapandiyan

Abstract

Bir kobay (Cavia porcellus) kolonisinde akut nitrat-nitrit
zehirlenmesine bağlı olarak 88 adetinden 28 adetinin spontan
olarak öldüğü vaka sunuldu. Cichorium intybus bitkisinin
yeşil yapraklarının yenmesine bağlı olarak nitrat-nitrit
zehirlenmesinin neden olduğunu ölümler düşünüldü. 28 adet
kobaydaki belirgin belirtiler ise doğal deliklerden akan çikolata
renkli kan, siyanotik mukoz membranlar, midriyazis ve şişmiş
karın olarak gözlendi. 28 hayvanın nekropsisinde büyük viseral
organlarda kanamalar, böbrekte tubuler nefroz, dalakta lenfoid
yapı, karaciğerde hidrofobik değişiklikler ile nekroz, barsaklarda
hemaraji ve akciğerde histopatalojik değişiklikler. Nitrat-nitrit
zehirlenmesi spektrofotometrik olarak doğrulandı. C. intybus
toksikolojik analizinde %2.1 g nitrat, %1.0 g nitrit ve 80 ppb
sitrinin varlığı belirlendi. Barsak boşluğu 10 ppm nitrat ve 1 ppm
nitrit içermekte olduğu belirlendi. Bilindiği kadarı ile mevcut
vaka, C. intybus yeşil yapraklarının yenmesine bağlı kobaylarda
akut nitrat-nitrit zehirlenmesinin bildirildiği ilk vakadır.

Keywords

Nitrat-nitrit zehirlenmesi, Cichorium intybus, kobay

An incidence of acute nitrate-nitrite poisoning in a Guinea pig (Cavia porcellus) colony

Abstract

We present an incidence of acute nitrate-nitrite poisoning in
a guinea pig (Cavia porcellus) colony which resulted in the
substantial and spontaneous death of 28 out of 88 guinea pigs.
The cause of death was traced to nitrate-nitrite toxicity from
ingestion of the green leaves of overfertilized Cichorium intybus.
The predominant symptoms in the 28 guinea pigs were chocolate
brown bloody discharge from all natural orifices, cyanotic
mucous membrane, mydriasis, and bloated abdomen. Necropsy
of all the 28 animals showed hemorrhage in all visceral organs
grossly, and tubular nephrosis of the kidney, lymphoid depletion
in the spleen, hydropic changes and necrosis in hepatocytes,
and hemorrhages in the intestine and lungs histopathologically.
Nitrate-nitrite toxicity was confirmed spectrophotometrically.
The toxicological analysis of the feed samples of C. intybus
revealed 2.1 g% nitrate, 1.0 g% nitrite, and 80 ppb citrinin. The
intestinal loop had 10 ppm of nitrate and 1 ppm of nitrite. To the
best of our knowledge, this is the first report of acute nitratenitrite
poisoning in guinea pigs owing to the ingestion of the
green leaves of overfertilized C. intybus.

Keywords

Nitrate-nitrite poisoning, Cichorium intybus, guinea pig

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