Küçük ölçekli süt işletmelerinde sığır mastitislerinden izole edilen Staphylococcus aureus suşlarında mecA, vanA ve pvl genlerinin araştırılması

Öz

Bu çalışmada sığır mastitislerinden Staphylococcus aureus'un izolasyonu, izole edilen suşlarda mecA, vanA ve pvl genlerinin varlığının araştırılması ve S. aureus suşlarının veteriner sahada yaygın olarak kullanılan bazı antibiyotiklere dirençliliklerinin belirlenmesi amaçlandı. Çalışmada, Afyonkarahisar'ın merkez kasaba ve köylerindeki 40 farklı küçük ölçekli süt işletmesinde bulunan 170 laktasyondaki ineğe ait 602 meme lobu süt örneği kullanıldı. CMT skorlamasının ardından, her bir meme lobundan aseptik olarak süt örnekleri toplandı. Çalışmada, ticari identifikasyon kiti kullanılarak 602 meme lobu süt örneğinden toplam 23 (%3,8) S. aureus suşu izole edildi. Suşların hiçbirinde mecA, vanA ve pvl genleri belirlenmedi. Tüm S. aureus suşlarının 12 antibiyotiğe karşı fenotipik dirençlilikleri Kirby-Bauer disk difüzyon yöntemi kullanılarak belirlendi. Suşlardaki en yüksek direnç oranı penisilin G'ye (%52,2) karşı iken, bunu oksasilin (%21,7), eritromisin (%21,7) ve amoksisilin+klavulanik aside (%17,4) karşı direnç oranları izledi. Sonuç olarak, S. aureus'un örneklemenin yapıldığı küçük ölçekli işletmeler için sığır mastitislerinin etiyolojisinde yaygın bir patojen olmadığı belirlendi.

Anahtar Kelimeler

• Mastitis
• metisilin direnci
• Panton-Valentine lökosidin
• S. aureus
• vankomisin direnci

Investigation of mecA, vanA and pvl genes in Staphylococcus aureus strains isolated from bovine mastitis in smallholder dairy farms

Öz

This study aimed to isolate the Staphylococcus aureus from bovine mastitis, investigate the presence of mecA, vanA and pvl genes in isolated strains and determine the antibiotic resistance of S. aureus strains to some antibiotics commonly used in veterinary field. In the present study, 602 mammary quarter milk samples belong to 170 lactating cows from 40 different smallholder dairy farms located center town and villages of Afyonkarahisar were used. After the CMT scoring, the milk samples were aseptically collected from each mammary quarter. In this study, a total of 23 (3.8%) S. aureus strains were isolated from 602 mammary quarter milk samples by using a commercial identification kit. The presence of mecA, vanA and pvl genes was found in none of the strains. Phenotypic resistances of all S. aureus strains to 12 antibiotics were investigated by using Kirby-Bauer disc diffusion method. The highest resistance rate in strains was against penicillin G (52.2%), followed by oxacillin (21.7%), erythromycin (21.7%) and amoxicillin+clavulanic acid (17.4%). In conclusion, it was noted that S. aureus was not a prevalent pathogen in the etiology of bovine mastitis for the smallholder farms sampled.

Anahtar Kelimeler

• Mastitis
• methicillin resistance
• Panton-Valentine leukocidin
• S. aureus
• vancomycin resistance

Kaynakça

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