Agents isolated from horses with respiratory system infection signs

Öz

The aim of this study is to isolate the agents from nasal swabs taken from horses with respiratory system findings and to examine the susceptibility of the isolated agents to various antibiotics.Nasal swab samples were taken from 30 horses of different age groups with respiratory system symptoms. The samples taken were cultured on Blood agar, MacConkey agar, Mycoplasma agar and Sabouraud Dexgrose agar. The properties of the colonies formed after being kept in appropriate incubation times and conditions were examined. Gram staining was applied and biochemical tests were performed for identification. In addition, antibiogram test was applied to isolated agents. Ten different agents were grown from nasal swabs taken from horses of different ages with respiratory system problems. While 2 different agents were isolated in two samples, pure agent was grown from 28 samples. As a result of the biochemical tests, 9 Streptococcus zooepidemicus (27.27%), 4 Staphylococcus aureus (12.12%), Staphylococcus epidermidis (12.12%), and Klebsiella spp. (12.12%), 3 Pasteurella spp. (9.09%), 2 Streptococcus pyogenes (6.06%), Bacillus sp. (6.06%), Corynebacterium spp. (6.06%), and Candida spp. (6.06%) and 1 Rhodococcus equi (3.03%) were identified. As a result of the antibiogram test, it was determined that the antibiotic to which the agents were most sensitive was Amoxicillin/clavulanic acid (84.84%) and the most resistant to Penicillin-G (15.15%). The most Streptococcus zooepidemicus (27.27%) and the least Rhodococcus equi (3.03%) were identified from nasal swab samples taken from 30 horses of different age groups with respiratory system problems. In invitro conditions, it was determined that the agents were most sensitive to Amoxicillin / clavulanic acid (84.84%) and resistant to Penicillin-G (15.15%).

Anahtar Kelimeler

• Amoxicillin/Clavulanci acid
• Antibiogram test
• Candida spp.
• Horse
• Streptococcus zooepidemicus

Solunum sistemi infeksiyonu semptomlu atlardan izole edilen etkenler

Öz

Bu çalışmanın amacı, solunum sistemi bulguları bulunan atlardan alınan nasal svaplardan etkenleri izole etmek ve izole edilen etkenlerin çeşitli antibiyotiklere duyarlılıklarını incelemektir. Solunum sistemi bulguları görülen farklı yaş grubunda bulunan 30 attan nasal svap örnekleri alındı. Alınan örnekler Kanlı agar, MacConkey agar, Mycoplasma agar ve Sabouraud Dexgrose agar’da kültüre edildi. Uygun inkubasyon süresi ve koşullarında tutulduktan sonra oluşan kolonilerin özellikleri incelendi. Gram boyama uygulandı ve identifikasyon için biyokimyasal testler yapıldı. Ayrıca izole edilen etkenlere antibiyogram testi uygulandı. Solunum sistemleri problemleri görülen farklı yaşlardan atlardan alınan nasal svaplardan 10 farklı etken üredi. İki örnekte 2 farklı etken izole edilirken 28 numuneden saf etken üredi. Yapılan biyokimyasallar sonucu 9 Streptococcus zooepidemicus (%27.27), 4 Staphylococcus aureus (%12.12), 4 Koagulaz negative Staphylococcus spp. (%12.12), Klebsiella spp. (%12.12), ve Pasteurella spp. (%9.09), 2 Streptococcus pyogenes (%6.06), Bacillus sp. (%6.06), Corynebacterium spp. (%6.06), ve Candida spp. (%6.06) ve 1 Rhodococcus equi (%3.03) identifiye edildi. Yapılan antibiyogram testi sonucu, etkenlerin en duyarlı olduğu antibiyotik, Amoxicillin/Clavulanic acid (%84.84), en dirençli olduğu ise Penicillin-G (%15.15) olarak belirlendi. Solunum sistemi problemi olan farklı yaş gruplarındaki 30 attan alınan nasal svap örneklerinden en fazla Streptococcus zooepidemicus (%27.27), en az ise Rhodococcus equi (%3.03) identifiye edildi. İnvitro koşullarda ise etkenlerin en fazla Amoxicillin /clavulanic acid (%84.84)’e duyarlı, Penicillin-G (%15.15)’ye ise dirençli olduğu belirlendi.

Anahtar Kelimeler

• Amoxicillin/Clavulanic Acid
• Antibiyogram test
• At
• Candida spp.
• Streptococcus zooepidemicus

Kaynakça

1. Ainsworth DM, Cheetham J. (2010) Disorders of the respiratory disease. Reed SM, Bayly WM, Sellon DC.eds. Equine Internal Medicine, 3th edition, SaundersElsevier, St. Louis, p. 290 – 371.