Effects of probiotic application on oviduct and ovarian morphology in quails under heat stress

Yıl 2022, , 89 - 96, 15.06.2022

Özge Yıldırım , Sadık Serkan Aydın , Ömer Korkmaz , Deniz Korkmaz , İsmail Demircioğlu , Nurcan Kırar , Şermin Top , Tuğra Akkuş , Birten Emre , Ali Tekçe

https://doi.org/10.35864/evmd.1105912

Cited By: 1

Öz

The aim of the present study is to investigate the morphological effect of heat stress on the ovaries and oviducts in quails, the organs where egg production and structuring take place, and to eliminate this effect with probiotics. For this purpose, 40 female laying Japanese quail (Coturnix coturnix Japonica) were randomly divided into 4 groups. The first group ambient temperature was kept at 14-25 °C all day for 8 weeks. During the quails in the second group were kept at 14-25 °C, fermented natural LAB (Lactic Acid Bacteria) liquid was added to the drinking water at a level of 0,5 cc/L. In the third group, heat stress was created so that the house environment was kept at 30-34 °C for 8-12 hours a day. The quails in the fourth group were exposed to heat stress for 8 weeks, and 0,5 cc/L fermented natural LAB liquid was added to the drinking water. At the end of this process, the number of follicles in the ovaries of all subjects was recorded. The diameters of the yellow follicles and the length and width of the oviduct sections were digitally measured in mm. As a result, a strong negative relationship was observed between the magnum and isthmus lengths of the oviduct parts of quails. It was measured that the number of yellowish follicles and the mean diameter of yellow follicles decreased in quails under heat stress. In quails exposed to heat stress, the application of fermented lactic acid bacteria with probiotic effects as feed additives has been shown to reduce the number of yellow follicles and prevent live weight loss.

Anahtar Kelimeler

Coturnix coturnix Japonica, Folliculogenesis, Heat Stress, Oviduct, Probiotic

Sıcaklık stresindeki bıldırcınlarda probiyotik uygulamasının ovidukt ve ovaryum morfolojisine etkileri

Öz

Sunulan çalışmanın amacı sıcaklık stresinin bıldırcınlarda, yumurta üretimi ve yapılandırılmasının gerçekleştiği organlar olan, ovaryum ve ovidukt üzerine morfolojik etkisi ve bu etkinin probiyotik ile giderilmesini araştırmaktır. Bu amaçla 40 adet dişi yumurtacı Japon bıldırcını (Coturnix coturnix Japonica) rastgele 4 gruba ayrıldı. Birinci grup ortam sıcaklığı 8 hafta boyunca tüm gün 14-25 °C’de tutuldu. İkinci gruptaki bıldırcınlar 14-25 °C’de tutulduğu süre boyunca fermente edilmiş doğal LAB (Laktik Asit Bakteri) sıvısı 0,5 cc/L düzeyinde içme suyuna ilave edildi. Üçüncü grupta kümes ortamı günde 8-12 saat 30-34 °C’de tutulacak şekilde sıcaklık stresi oluşturuldu. Dördüncü gruptaki bıldırcınlara sıcaklık stresine maruz bırakıldığı 8 hafta boyunca 0,5 cc/L düzeyinde fermente edilmiş doğal LAB sıvısı içme suyuna ilave edildi. Bu sürecin sonunda tüm deneklerin ovaryumlarındaki folikül sayıları kaydedildi. Sarı folikül çapları ve ovidukt bölümlerinin uzunluk ve genişlikleri dijital olarak mm cinsinden ölçüldü. Sonuç olarak bıldırcınlarda ovidukt bölümlerinden magnum ve isthmus uzunlukları arasında güçlü bir negatif ilişki gözlemlenmiştir. Sıcaklık stresi altındaki bıldırcınlarda sarımsı folikül sayısının ve ortalama sarı folikül çaplarının küçüldüğü ölçülmüştür. Sıcaklık stresine maruz bırakılan bıldırcınlarda, probiyotik etkili fermente laktik asit bakterilerin yem katkı maddesi olarak uygulanmasının sarı folikül sayısının azalmasını ve canlı ağırlık kaybını engellediği görülmüştür.

Anahtar Kelimeler

Coturnix coturnix Japonica, Folikülogenezis, Ovidukt, Probiyotik, Sıcaklık Stresi

Kaynakça

• Abdel-Moneim AME, Shehata AM, Khidr RE, Paswan VK, Ibrahim NS, El-Ghoul AA, Aldhumri SA, Gabr SA, Mesalam NM, Elbaz AM, Elsayed MA, Wakwak MM, Ebeid TA. (2021) Nutritional manipulation to combat heat stress in poultry–A comprehensive review. Journal of Thermal Biology, 98, 102915. doi:10.1016/j.jtherbio.2021.102915
• Adanır S. (2019) Sıcaklık stresine maruz kalan Japon bıldırcınlarında probiyotik kullanımının büyüme gelişme ve bazı kan parametrelerine olan etkisi. Yüksek Lisans Tezi, BÜ Fen Bilimleri Enstitüsü, Bingöl.
• Akbalık ME, Saruhan BG, Topaloğlu U, Ketani MA. (2016) Kanatlılarda genital sistem histolojisi. Dicle Üniversitesi Veteriner Fakültesi Dergisi, (2), 73-82.
• Alçay AÜ. (2019) İstanbul’da satılan pişmiş tavuk dönerlerin mikrobiyolojik kalitesinin araştırılması. Türk Mikrobiyoloji Cemiyeti Dergisi, 49(2), 74-85. doi:10.5222/TMCD.2019.074
• Anna H. (2021) Matrix Metalloproteinases (MMPs) and inhibitors of MMPs in the avian reproductive system: An Overview. International Journal of Molecular Sciences, 22(15), 8056. https://doi.org/10.3390/ijms22158056
• Arjona AA, Denbow DM, & Weaver Jr WD. (1988) Effect of heat stress early in life on mortality of broilers exposed to high environmental temperatures just prior to marketing. Poultry science, 67(2), 226-231.doi: 10.3382/ps.0670226
• Arslan A. (2012) Yoğun Yerleşim Sıklığında Beslenen Bıldırcınlarda Farklı Propolis Düzeylerinin Performans Karkas Yağ Asitleri ve Bazı Biyokimyasal Parametreler Üzerine Etkisi. Doktora Tezi, FÜ Sağlık Bilimleri Enstitüsü, Elazığ.
• Assersohn K, Brekke P, Hemmings N. (2021) Physiological factors influencing female fertility in birds. Royal Society Open Science, 8(7), 202274. doi: 10.1098/rsos.202274
• Aydın BD, Sezer Ç, Oral NB. (2008) Kars’ ta satışa sunulan süzme balların kalite niteliklerinin araştırılması. Kafkas Ünv Vet Fak Derg, 14(1), 89-94. doi:10.9775/kvfd.2008.12-A
• Bakst MR. (1998) Structure of the avian oviduct with emphasis on sperm storage in poultry. Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 282(4‐5), 618-626. doi:10.1002/(SICI)1097-010X(199811/12)282:4/5<618::AID-JEZ11>3.0.CO;2-M
• Batool F, Bilal RM, Hassan FU, Nasir TA, Rafeeque M, Elnesr SS, Farag MR, Mahgoub HAM, Naiel MAE, Alagawany M. (2021) An updated review on behavior of domestic quail with reference to the negative effect of heat stress. Animal Biotechnology, 1-14. doi: 10.1080/10495398.2021.1951281
• Brooks JC, Martinez B, Stratton J, Bianchini A, Krokstrom R, Hutkins R. (2012) Survey of raw milk cheeses for microbiological quality and prevalence of foodborne pathogens. Food Microbiology, 31(2), 154-158. doi: 10.1016/j.fm.2012.03.013
• Chao Y, Kim HW, Cramer T, Cheng HW, Kim YHB. (2018) Effect of probiotic feeding on oxidative stability and meat quality attributes of breast muscle from chickens exposed to chronic heat stress. Meat and Muscle Biology, 1(3). doi: 10.221751/rmc2017.161
• Cramer TA, Kim HW, Chao Y, Wang W, Cheng HW, Kim YHB. (2018) Effects of probiotic (Bacillus subtilis) supplementation on meat quality characteristics of breast muscle from broilers exposed to chronic heat stress. Poultry science, 97(9), 3358-3368. doi: 10.3382/ps/pey176
• Cruvinel JM, Urayama PMG, Dos Santos TS, Denadai JC, Muro EM, Dornelas LC, ... & Pezzato AC. (2021) Different dietary electrolyte balance values on performance, egg, and bone quality of Japanese quail (Coturnix Coturnix Japonica) under heat stress. Tropical Animal Health and Production, 53(1), 1-8. doi: 10.1007/s11250-020-02472-7
• De Melo Pereira GV, de Oliveira Coelho B, Júnior AIM, Thomaz-Soccol V, Soccol CR. (2018) How to select a probiotic? A review and update of methods and criteria. Biotechnology advances, 36(8), 2060-2076. doi: j.biotechadv.2018.09.003
• Dosoky WM, Zeweil HS, Ahmed MH, Zahran SM, Ali AM, Abdelsalam NR, Naiel MA. (2021) The influences of Tylosine and licorice dietary supplementation in terms of the productive performance, serum parameters, egg yolk lipid profile, antioxidant and immunity status of laying Japanese quail under heat stress condition. Journal of Thermal Biology, 99, 103015. doi: j.jtherbio.2021.103015
• Etches RJ, John TM, Gibbins AV. (2008) Behavioural, physiological, neuroendocrine and molecular responses to heat stress. Poultry Production in Hot Climates, Second edition. Ed. NJ Daghir. pp.31-66.
• Fathi MM, Ebeid TA, Al-Homidan I, Soliman NK, Abou-Emera OK. (2017) Influence of probiotic supplementation on immune response in broilers raised under hot climate. British poultry science, 58(5), 512-516. doi: 10.1080/00071668.2017.1332405
• Habibian M, Ghazi S, Moeini MM. (2016) Effects of dietary selenium and vitamin E on growth performance, meat yield, and selenium content and lipid oxidation of breast meat of broilers reared under heat stress. Biological trace element research, 169(1), 142-152. doi: 10.1007/s12011-015-0404-6
• Harrigan WF, McCance ME. (1976) Laboratory Methods in Food and Dairy Microbiology (No. QR 115. H37).
• Harrigan WF. (1998) Laboratory Methods in Food Microbiology. Third edition, Academic Press, San Diego
• Hrabia A. (2021) Matrix metalloproteinases (MMPs) and inhibitors of MMPs in the avian reproductive system: an overview. International Journal of Molecular Sciences, 22(15), 8056. doi: 10.3390/ijms22158056
• İnci H. (2019) Yumurtacı Bıldırcınlarda Sıcaklık Stresine Karşı Probiyotik Kullanımının Bazı Kan Parametreleri Üzerine Etkisi. Türk Tarım ve Doğa Bilimleri Dergisi, 6(4), 887-892. doi: 10.30910/turkjans.633628
• Jahromi MF, Altaher YW, Shokryazdan P, Ebrahimi R, Ebrahimi M, Idrus Z, ... & Liang JB. (2016) Dietary supplementation of a mixture of Lactobacillus strains enhances performance of broiler chickens raised under heat stress conditions. International Journal of Biometeorology, 60(7), 1099-1110. doi:10.1007/s00484-015-1103-x
• Johnson PA. (2012) Follicle selection in the avian ovary. Reproduction in domestic animals, 47, 283-287. doi: 10.1111/j.1439-0531.2012.02087.x
• Kaya A, Dama G. (2018) Yumurtacı Tavuk Rasyonlarına Farklı Düzeylerde Propiyonik Asit İlavesinin Performans, Yumurta Kalitesi ve Bazı Kan Parametreleri Üzerine Etkisi. Tekirdağ Ziraat Fakültesi Dergisi, 15(1), 129-134.
• Koyama H, Shi D, Fujimori T. (2019) Biophysics in oviduct: Planar cell polarity, cilia, epithelial fold and tube morphogenesis, egg dynamics. Biophysics and physicobiology, 16, 89-107. doi: 10.2142/biophysico.16.0_89
• Lott BD, Day EJ, Deaton JW, & May JD. (1992) The effect of temperature, dietary energy level, and corn particle size on broiler performance. Poultry science, 71(4), 618-624. doi:10.3382/ps.0710618
• Masuko T, Hariyama Y, Takahashi Y, Cao LM, Goto M, Ohshima M. (2002) Effect of addition of fermented juice of epiphytic lactic acid bacteria prepared from timothy and orchardgrass on fermentation quality of silages. Japanese Journal of Grassland Science, 48(2), 120-125. doi: 10.14941/grass.48.120_1
• Mofidi MR, Yousef EM, Lotf EH, Bagherzadeh KF, Dehghani MR. (2021) Effect of Dietary Electrolyte Balance and Probiotic on Performance and Carcass Characteristics of Japanese Quails Under Heat Stress Conditions. Research on Animal Production, 11(30), p.20-30.
• Omrak H. (2021) Türkiye kanatlı eti üretiminde dünyada 10. Sırada. 24.11.2021. Türk Tarım ve Orman e-dergi. Erişim linki: http://www.turktarim.gov.tr/Haber/702/turkiye-kanatli-eti-uretiminde-dunyada-10--sirada, Erişim tarihi: 23.03.2022.
• Onderci M, Sahin K, Sahin N, Cikim G, Vijaya J, Kucuk O. (2005) Effects of dietary combination of chromium and biotin on growth performance, carcass characteristics, and oxidative stress markers in heat-distressed Japanese quail. Biological Trace Element Research, 106(2), 165-176.
• Orhan C, Sahin N, Sahin K, Kucuk O. (2021) Influence of dietary genistein and polyunsaturated fatty acids on lipid peroxidation and fatty acid composition of meat in quail exposed to heat stress. Tropical Animal Health and Production, 53(5), 1-11. doi:10.1007/s11250-021-02933-7
• Saleh AA, Eltantawy MS, Gawish EM, Younis HH, Amber KA, El-Moneim A, Ebeid TA. (2020) Impact of dietary organic mineral supplementation on reproductive performance, egg quality characteristics, lipid oxidation, ovarian follicular development, and immune response in laying hens under high ambient temperature. Biological trace element research, 195(2), 506-514. doi: 10.1007/s12011-019-01861-w.
• Sen S, Ingale SL, Kim YW, Kim JS, Kim KH, Lohakare JD, ... & Chae BJ. (2012) Effect of supplementation of Bacillus subtilis LS 1-2 to broiler diets on growth performance, nutrient retention, caecal microbiology and small intestinal morphology. Research in Veterinary Science, 93(1), 264-268. doi: 10.1016/j.rvsc.2011.05.021
• Sert S, & Kıvanç M. (1985) Taze civil ve lor peynirleri üzerinde mikrobiyolojik çalışmalar. Gıda, 10(5), 287-292.
• Song J, Xiao K, Ke YL, Jiao LF, Hu CH, Diao QY, ... & Zou XT. (2014) Effect of a probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress. Poultry science, 93(3), 581-588. doi: 10.3382/ps.2013-03455
• Şentürk MG, Uyanık F. (2016) Yumurtacı Bıldırcınlarda Oluşturulan Isı Stresinde Krom ve Çinkonun Bazı Kan Parametrelerine Etkileri. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 13(1), 38-49. Tekçe E, Bayraktar B, Aksakal V, Dertli E, Kamiloglu A, Topcu KC, Yasulergezer N. (2020) Influence of Lactobacillus reuteri on internal organ weight, performance and meat quality of Japanese quail (Coturnix coturnix japonica) under heat stress. European Poultry Science. doi: 10.1399/eps.2020.304
• Tonbak F. (2012) Sıcaklık Stresine Maruz Bırakılan Bıldırcınlarda Rasyona İlave Edilen Tarçın Yağının Performans ve Bazı Kan Parametreleri Üzerine Etkileri. (Yüksek Lisans Tezi, FÜ Sağlık Bilimleri Enstitüsü, Elazığ)
• Türkiye İstatistik Kurumu. (2021) Hayvansal Üretim İstatistikleri. Erişim linki: https://data.tuik.gov.tr/Bulten/Index?p=Hayvansal-Uretim-Istatistikleri-Aralik-2021-45593 , Erişim tarihi: 26.03.2022.
• Wasti S, Sah N, Mishra B. (2020) Impact of heat stress on poultry health and performances, and potential mitigation strategies. Animals, 10(8), 1266. doi: 10.3390/ani10081266
• Zhang P, Yan T, Wang X, Kuang S, Xiao Y, Lu W, Bi D. (2017) Probiotic mixture ameliorates heat stress of laying hens by enhancing intestinal barrier function and improving gut microbiota. Italian Journal of Animal Science, 16(2), 292-300. doi:10.1080/1828051X.2016.1264261
• Zhang ZY, Jia GQ, Zuo JJ, Zhang Y, Lei J, Ren L, Feng DY. (2012) Effects of constant and cyclic heat stress on muscle metabolism and meat quality of broiler breast fillet and thigh meat. Poultry science, 91(11), 2931-2937. doi: 0.3382/ps.2012-02255