Changes in oxidative stress parameters in ear-tagged calves

Year 2020, Volume: 31 Issue: 2, 115 - 120, 31.12.2020

Mustafa Cellat Pınar Peker Akalın Cafer Tayer İşler Mehmet Güvenç İshak Gökçek Filiz Kazak Nuri Başpınar

https://doi.org/10.35864/evmd.763485

Abstract

The aim of this study was to investigate the effects of ear-tagging on blood malondialdehyde (MDA), reduced glutathione (rGSH), Vitamin C (Vit C) levels and glutathione peroxidase (GPx) activity. Ear-tagged (with polyurethane ear tag) animals consisted of 9 healthy Holstein calves (2-3 months old). Blood samples were taken just prior to ear-tagging (baseline), at the 20th min, on 3rd day and 8th day after the ear-tagging procedure. Six Holstein calves were involved for sham group. In ear-tagged animals, plasma MDA levels increased on the 3rd day compared to baseline values (p<0.01), and returned back to baseline values on the 8th day, whereas no change was found in sham group. In ear-tagged animals, plasma rGSH levels increased at the 20th min, 3rd day, and 8th day (p<0.001) in comparison to baseline values, which in sham group, there was no change. In ear-tagged animals, erythrocyte rGSH levels on the 3rd and 8th days were higher compared to baseline and 20th min level (p<0.01). In sham group, the 3rd and 8th day erythrocyte rGSH levels increased compared to baseline values (p<0.01). In ear-tagged animals, the 20th min, 3rd day and 8th day erythrocyte GPx activities were lower than the baseline values (p<0.001), whereas in sham group, no change was observed. While Vit C levels increased progressively in the sham group (p<0.05), this increase was not observed in the ear-tagged group. In conclusion, ear-tagging was found to cause oxidative stress and to increase antioxidant requirement in calves.

Keywords

calf, ear-tagging, oxidative stress, polyurethane ear tag

Kulak küpesi takılan buzağılarda oksidatif stres değişimleri

Abstract

Bu çalışmanın amacı kulak küpelemenin kan malondialdehid (MDA), redükte glutatyon (rGSH), vitamin C (Vit C) düzeyleri ile glutatyon peroksidaz (GPx) aktivitesi üzerine etkilerinin araştırılmasıdır. Çalışmanın deney grubunu, kulak küpeleme (poliüretan kulak küpesi ile) yapılan 9 adet buzağı (2-3 aylık yaşta) oluşturdu. Kan örnekleri kulak küpeleme işleminden hemen önce (temel seviye), kulak küpeleme sonrası 20. dakika, 3. gün ve 8. gün’de alındı. Ayrıca, belirtilen zamanlarda, sadece kanları alınan 6 adet Holstein ırkı buzağı da sham grubu olarak kullanıldı. Kulak küpeli buzağılarda, 3. gün MDA seviyeleri temel seviyelere göre yükselirken (p<0,01), 8. gün’de tekrar temel seviyelerine indi. Sham grubunda ise MDA düzeyleri yönünden bir değişiklik gözlenmedi. Kulak küpeleme grubunda plazma rGSH seviyeleri 20. dakika, 3. gün ve 8. gün’de temel düzeylere göre arttı (p<0.001) ancak sham gurubunda bir değişim gözlenmedi. Kulak küpeli buzağılarda eritrosit rGSH seviyeleri, 3. ve 8. günlerde, 20. dakika ve temel seviyelere göre yüksek olarak belirlendi (p<0.01). Sham grubunda ise 3. ve 8. gün eritrosit rGSH seviyeleri temel seviyelere nazaran yüksek olarak belirlendi (p<0.01). Kulak küpeleme grubunda, eritrosit GPx aktiviteleri 20. dakika, 3. gün ve 8. gün’lerde temel seviyelere kıyasla düşük (p<0.001) olmasına rağmen sham grubunda bir değişim göstermedi. Sham grubunda Vit C seviyeleri giderek artmasına rağmen (p<0.05), bu artış, kulak küpeli hayvanlarda görülmedi. Sonuç olarak kulak küpelemenin buzağılarda oksidatif strese neden olarak antioksidan ihtiyacı arttırdığı düşünüldü.

Keywords

buzağı, kulak küpeleme, oksidatif stres, poliüreatn kulak küpesi

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