The First Isolation and identification Of Miyagawanella Bovis from a bull semen

Year 1970, Volume: 3 Issue: 9-10, 53 - 73, 01.12.1970

Salih Yılmaz

Abstract

In this research work it has been suceeded to isoiate a virus
from PL V group on the embrioned egg culture of the fresh and
frozen semen which belonged to one of the Holstein breeding young
bulls with 333/64 ear-tag number raised on Karasu Cattle Breeding
Station, the blood serum of which had been found positive
in the Coınplement Fixation Test with the antigen of Enzootic Shecp
Abortion that was conducted to see whether the bull in question
carried or not the infecting agent of the · abovementioned disease.
1 - Further, the pathogeny tests were carried out on rats and
pregnant guinea pigs resulting in that the infecting agent has been
fixed on slides made from the liver of rats two weeks after the
ınfection; abc rtions have been observed in the infected guinea
pigs, and viral elementary bodies have been fixed in the aborted
foetuses.
2 - Positive reactions have been observed from the compleınent
fixation tests with the blood sera that obtained from the
~aid bull in differant intervals using the antigen of Enzootic Shecp
Abortion produced from the blood serum of the sheep which
had been prepared against the agent in question.
3 - The antiserum of Enzootic Sheep Abortion has neutralized
the virusthat had been produced from the semen of the bull
ın question; and, the antiserum against this agent that had been
produced from the sheep in question has neutralized the agent of
Enzöotic Sheep Abortion. This fact has been proved by crass-neu
traliza tion test.
4 - Results of indentification tests have led us in a belief
rhat the virus isolated successfu1ly from the embrioned egg culture
made by the bull semen first time in Turkey which belongs to
the Psittakosis Lymphogranuloma Venerum (PLV) group, is found
to be as «Miyagawanella Bovis» (M. Bovis).

Boğa Spermasından İlk Defa İzole ve İdantifiye Edilen «Miyagawanella Bovis»

Abstract

Bu araştırmada; kan senımu «Enzootik koyun abortüsü» antijeni
ile komplement - fixation test'inde müsbet reaksiyon veren
Karasu İnekhanesi damızlıklarından Holstein (Siyah-beyaz alaca)
ırkından 333/64 kulak numaralı genç bir boğanın enfeksiyon etkenini taşıyıp taşımadığı hususu araştırılmış olup taze dondurulmuş halde getirtilen boğaya ait sperma mayiinden embryonlu tavuk yumurta kültürlerinde PLV grubundan biy virus izolesine muvaffak olunmuştur.
1 - Laboratuvar deney hayvanlanndan fare ve gebe . kabaylarda
patojenite testleri yapılmış, enfeksiyondan iki hafta sonra
farelerin karaciğerlerinden yapılan preparatlarda ajan tesbit edilmiş, kabaylarda yavru atımı sağlanmış ve atık kobay cenin organlannda viral elementer cisimciklerin varlığı isbatlanmıştır.
2 - Enfekte boğadan çeşitli zamanlarda alınan kan serumları
ıle ajana karşı koyunda hazırlanan kan serumu Enzootik koyun
abartüsü antijeninin kullanıldığı komplement - fixation test'inde
müsbet reaksiyon vermişlerdir.
3 - Enzootik koyun abartüsü antiserun1u, boğa spermasından
izole edilen virusu, bu ajana karşı koyundan elde edilen antiserumda enzootik koyun abortüs etkenini nötralize etmişlerdir. Bu husus kros neutralisasyon test'leriyle saptanmıştır.
4 - ldantifikasyon çalışmaları sonucu; ilk defa yurdumuzda
boğa Sperma mayiinden embryonlu tavuk yumurta kültürlerinde
izolesine muvaffak olunan Psittakosis lymphogranuloma venereum (PLV) grubuna ait virusun «Miyagawanella bovis «(M. bovis) olduğu kanısını vermiştir.

Keywords

Boğa Sperması, Miyagawavenella Bovis, 1970, Salih YILMAZ

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