Studies on the preparation and antigenicity of the vaccine against leptospirosis.

Year 1979, Volume: 5 Issue: 1-2-3, 44 - 58, 01.12.1981

Ali Altan Bulu

Abstract

The vaccine was prepared fröm Leptospira grippotyphosa
Haymana strain which was isolated by the author from Haymana-
Ankara.
The culture was grawn in Chang's medium supplemented
with vitamin Bıı for 9 days.
Formelin (37 %) was added in the ratio of 0,05 % after harvesting
the culture. The number of the leptospira organisms
were counted with a haemocytometer and the culture was filtered
through types SM, 11305-0,65 pore size sartari us membrane.
Leptospira organisms on the filter membrane we.re collected
in to a steril flask at concentration of 1,5 x 109 /ml. with saline
solution containing 0,01 % merthiolate·.
The monovalent vaccine was produced from Haymana-strain.
CL. grippotyphosa) purity of the vaccine was ascertained in
nutrient broth, blood agar (aerobic and anaerobic). Fletcher's
agar, Chang medium, Littman Oxgall agar, mycobiotic agar and
Sabourod's medium.
Safety of the vaccine was checkedin goats, rabbits, guinea
pigs and hamster.s
InnocuÜy of the vaccine was alsa checked by inoculating
two guinea pigs with 5 ml. of the vaccine.
For untowards reaction vaccinated animals were obserwed
for a period of 21 days. At 21 post vaccination day blood samples
from the tes animals were tested by micro agglutination Lysis
test. and titres were as follows:
Two goats 1/100, one· rabbit 1/200, two 1/800, two 1/1600
and guineo-pigs 1/100- 1/800.
The efficeacy of the vaccine w as tes te d by chaJlenging. vaccinated
and 12 control hamsters at 21 post vaccination day with
a virulent strain of L. grippotyphosa.
All of the control hamsters died within 14 days wheras the
vaccinated animals survived.

Keywords

Studies, preparation, antigenicity

Leptospirosis'e Karşı Etkin Bir Aşı Hazırlanması ilee Bu Aşının Keçi, Tavşan, Kobay Ve Hamsterlerde Bağışıklık Denemeleri

Abstract

Bu çalışma Ülkemizde u~un zamandır eksikliği duyulan
leptospirosis hastalığına karşı bir aşı hazırlamak için yapıldı.
Aşımızda Ankara- Haymadan izole ettiğimiz, patojen L. grippotyphosa
sero grubundan yerel haymana suşu kullanıldı.
Aşı üretimi Bıı vitamini katılmış Chang vasatında yapıldı.
Üretimden sonra % 0,05 formone inaktive edildi.
Aşı süzüldükten sonra 1,5 x 10-ıı germ olacak şekilde % 0,01
lik merthioletli fizyolojik tuzlu su ile sulandırıldı. Aşı mono:..
valan, formolle· inaktive edilmiş bir aşıdır. Sterilite kontrolunda
aşınan saf ve temiz oJduğu saptandı. Bağışıklık testlerinden
M.A testi ile keçi, tavşan, kabaylatın kan serumları işlendi. Yeterli
seviyede immun antikdrlar bulundu. Aynı hayvanlarda
aktif protektif test uygulandı. % 69 -% 84,5 arasında bağışıklık
sağlandı.

Keywords

Leptospirosis, Leptospirosis'e karşı Aşı, Leptospirosis Aşısı, Keçi, Tavşan, Kobay, Hamster, A. Altan BULU, 1979, 1980, 1981

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