Ankara Bölgesinde sığırlar arasında seyreden ikterohemoglobinuri vakalarında serolojik ve kültürel metotlarla tesbit edilen leptospirosis olayları

A. Altan Bulu; Mübeccel Yumuşak

Araştırma Makalesi

The studies on icterohaemorrhagio leptospiral infections of cattle in Ankara region.

Yıl 1979, Cilt: 5 Sayı: 1-2-3, 78 - 85, 01.12.1981

Öz

Blood samples were obtained from one of the 65 cattle
having fever and icterrohaemoglobinuriae atafarmin Haymana,
Ankara.
Its Blood and urine were inoculated into six-week old hamsters
by the subcutaneous route a:O.d into Fletchers, aıl"d Chang
Media.
After 29 days of ineubatian a strain was isolated in Fletcher's
medium.
Bovin urine inoculeted into hamster, leptospira were found
in t~ir kidney and isolated a new strain The strain were found
pozitive in M.A. test against leptospira grippotyphosa irnmun
serum in 1/~400 titre when sera from the 65 cattle tested examination
by M.A test gave pozitive result against Leptospira grippotyphosa
in 1/ 200-1/3200 titre.
From these cases, three cows belonging to this form died.
Case II
Blood serum and kidney samples from two cattle sent to our
laboratory f:rom Keskin, Ankara were.examined for leptospirosis.
Serum samples were examined by Micro Agglutination test
CM.AJ the sera were found positive against Leptospira grippotyphosa
in 1/1600 titre.
Tissue emulsions of kidneys were inoculated into Fletcher's,
and Chang media and to 6 weeks old 6 hamsters, subcutaneously
and intra peritoneally.
The leptospira organism were isolated in Fletcher's and
Chang media and were identified under the dark field microscope.
The two hamsters which were inoculated intra peritoneally
died within 13 days after inoculation kidney emufsion ·from
the dead hamsters were inoculacted into anather set off hamsters
for the pathogenicty control.
The isol'ated new strain was positive in, 1/ 10.000 titre against
Leptospira grippotyphosa immun serum when tested with M.A
test.

Kaynakça

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