Etlik Hayvan Hastalıkları Araştırma Enstitüsünde Gelen Kanatlı Materyallerinde Koligranuloma'nın Organsal Dağılımı ve Patolojik Bulguları

Year 1993, Volume: 7 Issue: 4, 146 - 171, 01.12.1993

Öznur Yazıcıoğlu

Abstract

Bu çalışmada, Etlik Hayvan Hastalıklan Araştırma Enstitüsüne gelen kanatlı
materyallerinde; koligranuloma olgularının görülme sıklığının, yerleşim
gösterdiği organların ve patolojik bulgularının saptanması amaçlanınıştır. 2 yıl
süren çalışma boyunca hastalık, sadece tavuklarda gözlendi ve incelenen 853
kartatlı materyalinin 14'ünde histolojik olarak saptandı. Koligranuloma, 14 olgunun
2'sinde 14-15 günlük civcivlerde görüldü. %1.64 gibi düşük bir prevalans bulundu.
Makroskobik bulgular, histolojik olarak koligranuloma tanısı konan 14 olgudan
9'unda görüldü. Bu bulgular, karaciğer, akciğer ve böbrekte; organların
visceral yüzlerinde parankime gömülü ya da seröz yüzeyden hafif çıkıntı
yapan az ya da çok sayıda, toplu iğne başından birkaç mm çapına kadar
değişen boyutlarda, grimsi-beyaz veya sarımsı renkte kabartılar şeklindeydi.
Ayrıca akciğer ve karaciğerde , fokal ya da diffuz nekroz alanlarına da rastlandı.
Bir olguda, bağırsak duvarında 1,5 x 0.5 x 0.9 cm boyutunda bir nodül
görüldü. Histolojik olarak granulomatöz lezyorıların görüldüğü 5 olguda,
makroskobik bir bulgu gözlenmedi.
Histolojik olarak granülomatöz lezyonlar bağırsak. karaciğer, akciğer,
böbrek, bursa fabrisius ve beyinde görüldü. Karaciğer ve bursa fabrisiusun en
sık etkilenen organlar olduğu dikkati çekti.
Mikroskobik olarak, erken lezyonların az ya da çok çekirdek debrileri kapsayan
ve etrafı epitelioid histiyositler ve çok çekirdekli dev hücreler tarafından
çevrilen fokal kazeifikasyon nekrozu alanlarından ibaret olduğu görüldü. Eski
lezyonlarda, tanımlanan bu lezyonun dışında çok sayıda lenfositler, makrofajlar,
az sayıda heterofiller ve plazma hücreleri ile infiltre bağdoku ve kapillar
damar proliferasyonu vardı. Ayrıca sentral nekroz alanlarında parçalanma ve
lamellasyon dikkati çekti fakat kalsifikasyon görülmedi. Daha eski lezyonların,
ince bir libröz kapsül tarafından çevrilcliği gözlendi.
Koligranuloma tanısı ; lezyonların morfolojik görünümüne ve MacCallum
Goodpasture Gram boyama tekniği ile boyanan kesitlerde, nekrotik alanlar
içinde Gram (-) bakterilerin görülmesine dayanarak kondu. Şüpheli materyaller,
tüberküloz yönünden Ziehl-Neelsen ve mikotik granulomalar yönünden
Grocott mantar boyası ile boyandı. E.coli, bakteriyolojik ekim yapılan 4 materyalin
sadece birinde karaciğerde görülen lezyonlardan izole edildi.

Keywords

Kanatlı, Escherichia coli, Kronik Granülomatöz Yangısı, Koligranuloma, Koligranuloma'nın Organsal Dağılımı, Öznur YAZICIOĞLU, Etlik Hayvan Hastalıkları Araştırma Enstitüsü, Etlik Veteriner Mikrobiyoloji Dergisi, 1993

«Determination of pathological findings and distribution of Coligranuloma in organs in poultry materials that were sent to Etlik Animal Diseases Research Institute»

Abstract

" Determination of patholojical findings and distribution of Coligranuloma
in .organs in poultry materials that sent to Etlik Animal Diseases.Research Institute"
In this study, it was aimed to determine the prevalence and involving organs
and patholojical findings of coligranuloma in poultry materials that sent
to Etlik Animal Diseases Research Institute. During the period of 2 years, it
was observed only in chickens and diagnosed histologically in 14 of 853 materials.
Coligranuloma was seenin 14-15 day-old chicks in 2 of 14 cases. The
prevalence w as as lo w as %1 ,64.
Macroscopical findings were seen in 9 of 14 cases that diagnosed coligranuloma
histologically. These were consist of a few or many, grayish-white or
yellowish, firm nodules varying from pin head to several mm in diameter penetrating
the paranchyma or protruding slightly on visceral surfaces of liver,
lung and kidney. In addition. focal or diffuse necrosis areas were found in
liver and lung. A nodule with 1,5 x 0,5 x 0,9 cm in diameter was seen on the
intestinal wall in one case. Macroscopical findings were not observed in 5 cases that had granulomatous lesions in histolojical examination.
Histologically, granulomatous lesion were seen on the intestine, liver,
lung, kidney, bursa fabricius and brain. The liver and bursa fabricius were affected
frequently.
Microscopically, early lesions were consist of focal areas of caseous necrosis
containing more or less nuclear debris and surrounding by a zone of epithelioid
histiocytes and multinucleated giant cells. In old lesions, it was seen
that connective tissue and kapillar vessel proliferations infiltrated with a large
number of lymphocytes, macrophages, a few heterophils and plasma cells
around the giant cells zone were present. However, fragmentation and lamellation
were seen in central necrosis areas but calcification was not. A thin fibrouse
tissue capsule was also evident in old er lesions.
The diagnosis of coligranuloma was carried out according to morphological
appearance of lesions and demonstration of Gran1 (-) organisms within necrotic
areas in stained sections w ith MacCallum Good pasture Gram's stain. Suspected
materials was stained with Ziehl-Neelsen for tuberculosis and Grocott
fungal stain for micotik granulomas. E.coli was isolated from lesions seen on
li ver in only one of four materials.

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