Investigation of Ruminant Encephalitic and Septicemic Listeriosis by the Immunofluorescence Method

Year 2021, Volume: 32 Issue: 1, 33 - 39, 30.06.2021

Emin Karakurt Fatih Büyük Serpil Dağ Enver Beytut Özgür Çelebi Özhan Karataş Hilmi Nuhoğlu Ali Sefa Mendil Doğan Akça Ayfer Yıldız

https://doi.org/10.35864/evmd.748570

Abstract

Listeria genus are Gram positive facultative intracellular bacteria that are found in the soil as saprophytes but also contain species that have pathogenic effects in their last host. Listeria monocytogenes is the major pathogen in Listeria species that is responsible for the majority of Listeriosis cases in humans and animals. In this study, it was aimed to investigate L. monocytogenes with histopathological and immunofluorescence methods in brain and liver tissues taken from sheep and cattle with clinical signs of suspicious Listeriosis. In the study, isolation and identification of L. monocytogenes with cultural methods were also applied. The material of the study consisted of 16 sheep and 2 bovine tissue samples. As a result of cultural analysis, L. monocytogenes was identified from 12 (66.6%) of the samples. Within the framework of histopathological and macroscopic findings, 16 (88.8%) of 18 cases with suspected Listeriosis were positive with the immunofluorescence method. These findings reveal that the immunofluorescent staining method is a reliable and sensitive method in the diagnosis of Listeriosis.

Keywords

Cattle, immunofluorescence, listeriosis, sheep

Ruminantların Ensefalitik ve Septisemik Listeriyozisinin İmmunofloresan Metotla Araştırılması

Abstract

Listeria cinsi toprakta saprofit olarak bulunan ancak son konaklarında patojen etki gösteren türleri de barındıran Gram pozitif fakültatif intraselüler bakterilerdir. Patojen Listeria türleri arasında Listeria monocytogenes ilk sırada yer alır ve insan ve hayvanlardaki Listeriyozis olgularının büyük bir kısmından sorumludur. Bu çalışmada, Listeriyozis şüpheli klinik belirti gösteren koyun ve sığırlardan alınan beyin ve karaciğer dokularında L. monocytogenes’in histopatolojik ve immunofloresan metodlarla araştırılması amaçlanmıştır. Çalışmada ayrıca L. monocytogenes’in kültürel yöntemlerle izolasyonu ve identifikasyonuna da başvurulmuştur. Çalışmanın materyalini, 16 koyun ve 2 sığıra ait doku örnekleri oluşturmuştur. Kültürel analizi sonucu örneklerin 12 (66,6%)’undan L. monocytogenes identifiye edilmiştir. Histopatolojik ve makroskobik bulgular çerçevesinde Listeriyozis şüpheli 18 vakanın 16 (88,8%)’si immunfloresan metodla pozitif tespit edilmiştir.
Bu bulgular, immunofloresan boyama yönteminin Listeriyozisin teşhisinde güvenilir ve hassas bir yöntem olduğunu ortaya koymaktadır.

Keywords

İmmunofloresan, koyun, listeriyozis, sığır

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