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Investigation of Ruminant Encephalitic and Septicemic Listeriosis by the Immunofluorescence Method

Year 2021, Volume: 32 Issue: 1, 33 - 39, 30.06.2021
https://doi.org/10.35864/evmd.748570

Abstract

Listeria genus are Gram positive facultative intracellular bacteria that are found in the soil as saprophytes but also contain species that have pathogenic effects in their last host. Listeria monocytogenes is the major pathogen in Listeria species that is responsible for the majority of Listeriosis cases in humans and animals. In this study, it was aimed to investigate L. monocytogenes with histopathological and immunofluorescence methods in brain and liver tissues taken from sheep and cattle with clinical signs of suspicious Listeriosis. In the study, isolation and identification of L. monocytogenes with cultural methods were also applied. The material of the study consisted of 16 sheep and 2 bovine tissue samples. As a result of cultural analysis, L. monocytogenes was identified from 12 (66.6%) of the samples. Within the framework of histopathological and macroscopic findings, 16 (88.8%) of 18 cases with suspected Listeriosis were positive with the immunofluorescence method. These findings reveal that the immunofluorescent staining method is a reliable and sensitive method in the diagnosis of Listeriosis.

References

  • Referans1al-Dughaym AM, Elmula AF, Mohamed GE, Hegazy AA, Radwan YA, Housawi FM, Gameel AA. (2001) First report of an outbreak of ovine septicaemic listeriosis in Saudi Arabia. Rev Sci Tech. 2001; 20, 777-783.
  • Referans2Barkallah M, Gharbi Y, Hmani M, Mallek Z, Gautier M, Gdoura R, Fendri I. (2016) Locked nucleic acid probe-based real-time PCR for the diagnosis of Listeria monocytogenes in ruminants. Mol Cell Probes. 30, 138-145.
  • Referans3Brugère-Picoux J. (2008) Ovine listeriosis. Small Ruminant Res. 76, 12-20.
  • Referans4Campero CM, Odeón AC, Cipolla AL, Moore DP, Poso MA, Odriozola E. (2002) Demonstration of Listeria monocytogenes by immunohistochemistry in formalin-fixed brain tissues from natural cases of ovine and bovine encephalitis. J Vet Med B Infect Dis Vet Public Health. 49, 379-383.
  • Referans5Çeribaşı S, Kızıl Ö, Karahan M. (2013) Listeriyozisli koyunlarda klinik, patolojik ve mikrobiyolojik bulgular. F Ü Sağ Bil Vet Derg. 27, 1-5.
  • Referans6Chen JQ, Regan P, Laksanalamai P, Healey S, Hu Z. (2017) Prevalence and methodologies for detection, characterization and subtypingof Listeria monocytogenes and L. ivanovii in foods and environmental sources. FSHW. 6, 97-120.
  • Referans7Dag S, Akca D, Karaman D, Celebi O, Ozen H, Buyuk F, Sahin M. (2013) Investigation of immunoperoxidase technique in smears prepared from vaginal secretions in use of early diagnosis of listerial abortions in cattle. Kafkas Univ Vet Fak Derg. 19, 1-6.
  • Referans8Dons L, Jin Y, Kristensson K, Rottenberg ME. (2007) Axonal transport of Listeria monocytogenes and nerve-cell-induced bacterial killing. J Neurosci Res. 85, 2529-2537.
  • Referans9Dramsi S, Lévi S, Triller A, Cossart P. (1998) Entry of Listeria monocytogenes into neurons occurs by cell-to-cell spread: an in vitro study. Infect Immun. 66, 4461-4468.
  • Referans10Dreyer M, Thomann A, Böttcher S, Frey J, Oevermann A. (2015) Outbreak investigation identifies a single Listeria monocytogenes strain in sheep with different clinical manifestations, soil and water. Vet Microbiol. 179, 69-75.
  • Referans11Fairley RA, Pesavento PA, Clark RG. (2012) Listeria monocytogenes infection of the alimentary tract (enteric listeriosis) of sheep in New Zealand. J Comp Pathol. 146, 308-313.
  • Referans12Food And Drug Administration (FDA). (2015) Testing methodology for Listeria species or L. monocytogenes in environmental samples. Version 1, Pp.2-11.
  • Referans13Gasanov U, Hughes D, Hansbro PM. (2005) Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: A review. FEMS Microbiol Rev. 29, 851-875.
  • Referans14Haligur M, Aydogan A, Ozmen O, Ipek V. (2019) Immunohistochemical evaluation of natural cases of encephalitic listeriosis in sheep. Biotech Histochem. 94, 341-347.
  • Referans15Headley SA, Bodnar L, Fritzen JT, Bronkhorst DE, Alfieri AF, Okano W, Alfieri AA. (2014) Histopathological and molecular characterization of encephalitic listeriosis in small ruminants from northern Paraná, Brazil. Braz J Microbiol. 44, 889-896.
  • Referans16Henke D, Rupp S, Gaschen V, Stoffel MH, Frey J, Vandevelde M, Oevermann A. (2015) Listeria monocytogenes spreads within the brain by actin-based intra-axonal migration. Infect Immun. 83, 2409-2419.
  • Referans17İlhan F, Ulusoy Y, Halıgür M. (2012) Matrix metalloproteinase expression in sheep with listerial meningoencephalitis. Res Vet Sci. 92, 269-272.
  • Referans18Johnson GC, Fales WH, Maddox CW, Ramos-Vara JA. (1995) Evaluation of laboratory tests for confirming the diagnosis of encephalitic listeriosis in ruminants. J Vet Diagn Invest. 7, 223-228.
  • Referans19Kaur S, Malik SV, Bhilegaonkar KN, Vaidya VM, Barbuddhe SB. (2010) Use of a phospholipase-C assay, in vivo pathogenicity assays and PCR in assessing the virulence of Listeria spp. Vet J. 184, 366-370.
  • Referans20Langohr IM, Ramos-Vara JA, Wu CC, Froderman SF. (2006) Listeric meningoencephalomyelitis in a cougar (Felis concolor): Characterization by histopathologic, immunohistochemical, and molecular methods. Vet Pathol. 43, 381-383.
  • Referans21Ligios C, Viglietti A, Carta P, Dexter G, Agrimi U, Simmons MM. (2004) Clinicopathological findings in sheep from Sardinia showing neurological signs of disease. Vet Rec. 154, 365-370.
  • Referans22Liu D, Ainsworth AJ, Austin FW, Lawrence ML. (2004) PCR detection of a putative N-acetylmuramidase gene from Listeria ivanovii facilitates its rapid identification. Vet Microbiol. 101, 83-89.
  • Referans23Loeb E. (2004) Encephalitic listeriosis in ruminants: Immunohistochemistry as a diagnostic tool. J Vet Med A Physiol Pathol Clin Med. 51, 453-455.
  • Referans24Low JC, Donachie W. (1997) A review of Listeria monocytogenes and listeriosis. Vet J. 153, 9-29.
  • Referans25McClain D, Lee WH. (1988) Development of USDA-FSIS method for isolation of Listeria monocytogenes from raw meat and poultry. J Assoc Off Anal Chem. 71, 660-664.
  • Referans26Nightingale KK, Schukken YH, Nightingale CR, Fortes ED, Ho AJ, Her Z, Grohn YT, McDonough PL, Wiedmann M. (2004) Ecology and transmission of Listeria monocytogenes infecting ruminants and in the farm environment. Appl Environ Microbiol. 70, 4458-4467.
  • Referans27Oevermann A, Di Palma S, Doherr MG, Abril C, Zurbriggen A, Vandevelde M. (2010) Neuropathogenesis of naturally occurring encephalitis caused by Listeria monocytogenes in ruminants. Brain Pathol. 20, 378-390.
  • Referans28Ortatatlı M, Çiftçi MK, Tuzcu M. (2001) Bir koyun sürüsünde görülen purulent ensefalitis. Vet Bil Derg. 17, 97-102.
  • Referans29Özyıldız Z, Dinçel GÇ, Terzi OS, Özsoy ŞY, Kul O. (2018) Immunohistochemical investigation of the damage to and repair of myelin, and astrocyte activity in small ruminants resulting from with natural meningoencephalitic listeriosis. Ankara Üniv Vet Fak Derg. 65, 283-290.
  • Referans30Ralovich B. (1993) Detection and epidemiological typing of Listeria strains – diagnostic methods for Listeria infections (A review). Acta Microbiol Hung. 40, 3-38.
  • Referans31Rocha PR, Lomonaco S, Bottero MT, Dalmasso A, Dondo A, Grattarola C, Zuccon F, Iulini B, Knabel SJ, Capucchio MT, Casalone C. (2013) Ruminant rhombencephalitis-associated Listeria monocytogenes strains constitute a genetically homogeneous group related to human outbreak strains. Appl Environ Microbiol. 79, 3059-3066.
  • Referans32Weinstock D, Horton SB, Rowland PH. (1995) Rapid diagnosis of Listeria monocytogenes by immunohistochemistry in formalin-fixed brain tissue. Vet Pathol. 32, 193-195.
  • Referans33Wesley IV, Larson DJ, Harmon KM, Luchansky JB, Schwartz AR. (2002) A case report of sporadic ovine listerial menigoencephalitis in Iowa with an overview of livestock and human cases. J Vet Diagn Invest. 14, 314-321.

Ruminantların Ensefalitik ve Septisemik Listeriyozisinin İmmunofloresan Metotla Araştırılması

Year 2021, Volume: 32 Issue: 1, 33 - 39, 30.06.2021
https://doi.org/10.35864/evmd.748570

Abstract

Listeria cinsi toprakta saprofit olarak bulunan ancak son konaklarında patojen etki gösteren türleri de barındıran Gram pozitif fakültatif intraselüler bakterilerdir. Patojen Listeria türleri arasında Listeria monocytogenes ilk sırada yer alır ve insan ve hayvanlardaki Listeriyozis olgularının büyük bir kısmından sorumludur. Bu çalışmada, Listeriyozis şüpheli klinik belirti gösteren koyun ve sığırlardan alınan beyin ve karaciğer dokularında L. monocytogenes’in histopatolojik ve immunofloresan metodlarla araştırılması amaçlanmıştır. Çalışmada ayrıca L. monocytogenes’in kültürel yöntemlerle izolasyonu ve identifikasyonuna da başvurulmuştur. Çalışmanın materyalini, 16 koyun ve 2 sığıra ait doku örnekleri oluşturmuştur. Kültürel analizi sonucu örneklerin 12 (66,6%)’undan L. monocytogenes identifiye edilmiştir. Histopatolojik ve makroskobik bulgular çerçevesinde Listeriyozis şüpheli 18 vakanın 16 (88,8%)’si immunfloresan metodla pozitif tespit edilmiştir.
Bu bulgular, immunofloresan boyama yönteminin Listeriyozisin teşhisinde güvenilir ve hassas bir yöntem olduğunu ortaya koymaktadır.

References

  • Referans1al-Dughaym AM, Elmula AF, Mohamed GE, Hegazy AA, Radwan YA, Housawi FM, Gameel AA. (2001) First report of an outbreak of ovine septicaemic listeriosis in Saudi Arabia. Rev Sci Tech. 2001; 20, 777-783.
  • Referans2Barkallah M, Gharbi Y, Hmani M, Mallek Z, Gautier M, Gdoura R, Fendri I. (2016) Locked nucleic acid probe-based real-time PCR for the diagnosis of Listeria monocytogenes in ruminants. Mol Cell Probes. 30, 138-145.
  • Referans3Brugère-Picoux J. (2008) Ovine listeriosis. Small Ruminant Res. 76, 12-20.
  • Referans4Campero CM, Odeón AC, Cipolla AL, Moore DP, Poso MA, Odriozola E. (2002) Demonstration of Listeria monocytogenes by immunohistochemistry in formalin-fixed brain tissues from natural cases of ovine and bovine encephalitis. J Vet Med B Infect Dis Vet Public Health. 49, 379-383.
  • Referans5Çeribaşı S, Kızıl Ö, Karahan M. (2013) Listeriyozisli koyunlarda klinik, patolojik ve mikrobiyolojik bulgular. F Ü Sağ Bil Vet Derg. 27, 1-5.
  • Referans6Chen JQ, Regan P, Laksanalamai P, Healey S, Hu Z. (2017) Prevalence and methodologies for detection, characterization and subtypingof Listeria monocytogenes and L. ivanovii in foods and environmental sources. FSHW. 6, 97-120.
  • Referans7Dag S, Akca D, Karaman D, Celebi O, Ozen H, Buyuk F, Sahin M. (2013) Investigation of immunoperoxidase technique in smears prepared from vaginal secretions in use of early diagnosis of listerial abortions in cattle. Kafkas Univ Vet Fak Derg. 19, 1-6.
  • Referans8Dons L, Jin Y, Kristensson K, Rottenberg ME. (2007) Axonal transport of Listeria monocytogenes and nerve-cell-induced bacterial killing. J Neurosci Res. 85, 2529-2537.
  • Referans9Dramsi S, Lévi S, Triller A, Cossart P. (1998) Entry of Listeria monocytogenes into neurons occurs by cell-to-cell spread: an in vitro study. Infect Immun. 66, 4461-4468.
  • Referans10Dreyer M, Thomann A, Böttcher S, Frey J, Oevermann A. (2015) Outbreak investigation identifies a single Listeria monocytogenes strain in sheep with different clinical manifestations, soil and water. Vet Microbiol. 179, 69-75.
  • Referans11Fairley RA, Pesavento PA, Clark RG. (2012) Listeria monocytogenes infection of the alimentary tract (enteric listeriosis) of sheep in New Zealand. J Comp Pathol. 146, 308-313.
  • Referans12Food And Drug Administration (FDA). (2015) Testing methodology for Listeria species or L. monocytogenes in environmental samples. Version 1, Pp.2-11.
  • Referans13Gasanov U, Hughes D, Hansbro PM. (2005) Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: A review. FEMS Microbiol Rev. 29, 851-875.
  • Referans14Haligur M, Aydogan A, Ozmen O, Ipek V. (2019) Immunohistochemical evaluation of natural cases of encephalitic listeriosis in sheep. Biotech Histochem. 94, 341-347.
  • Referans15Headley SA, Bodnar L, Fritzen JT, Bronkhorst DE, Alfieri AF, Okano W, Alfieri AA. (2014) Histopathological and molecular characterization of encephalitic listeriosis in small ruminants from northern Paraná, Brazil. Braz J Microbiol. 44, 889-896.
  • Referans16Henke D, Rupp S, Gaschen V, Stoffel MH, Frey J, Vandevelde M, Oevermann A. (2015) Listeria monocytogenes spreads within the brain by actin-based intra-axonal migration. Infect Immun. 83, 2409-2419.
  • Referans17İlhan F, Ulusoy Y, Halıgür M. (2012) Matrix metalloproteinase expression in sheep with listerial meningoencephalitis. Res Vet Sci. 92, 269-272.
  • Referans18Johnson GC, Fales WH, Maddox CW, Ramos-Vara JA. (1995) Evaluation of laboratory tests for confirming the diagnosis of encephalitic listeriosis in ruminants. J Vet Diagn Invest. 7, 223-228.
  • Referans19Kaur S, Malik SV, Bhilegaonkar KN, Vaidya VM, Barbuddhe SB. (2010) Use of a phospholipase-C assay, in vivo pathogenicity assays and PCR in assessing the virulence of Listeria spp. Vet J. 184, 366-370.
  • Referans20Langohr IM, Ramos-Vara JA, Wu CC, Froderman SF. (2006) Listeric meningoencephalomyelitis in a cougar (Felis concolor): Characterization by histopathologic, immunohistochemical, and molecular methods. Vet Pathol. 43, 381-383.
  • Referans21Ligios C, Viglietti A, Carta P, Dexter G, Agrimi U, Simmons MM. (2004) Clinicopathological findings in sheep from Sardinia showing neurological signs of disease. Vet Rec. 154, 365-370.
  • Referans22Liu D, Ainsworth AJ, Austin FW, Lawrence ML. (2004) PCR detection of a putative N-acetylmuramidase gene from Listeria ivanovii facilitates its rapid identification. Vet Microbiol. 101, 83-89.
  • Referans23Loeb E. (2004) Encephalitic listeriosis in ruminants: Immunohistochemistry as a diagnostic tool. J Vet Med A Physiol Pathol Clin Med. 51, 453-455.
  • Referans24Low JC, Donachie W. (1997) A review of Listeria monocytogenes and listeriosis. Vet J. 153, 9-29.
  • Referans25McClain D, Lee WH. (1988) Development of USDA-FSIS method for isolation of Listeria monocytogenes from raw meat and poultry. J Assoc Off Anal Chem. 71, 660-664.
  • Referans26Nightingale KK, Schukken YH, Nightingale CR, Fortes ED, Ho AJ, Her Z, Grohn YT, McDonough PL, Wiedmann M. (2004) Ecology and transmission of Listeria monocytogenes infecting ruminants and in the farm environment. Appl Environ Microbiol. 70, 4458-4467.
  • Referans27Oevermann A, Di Palma S, Doherr MG, Abril C, Zurbriggen A, Vandevelde M. (2010) Neuropathogenesis of naturally occurring encephalitis caused by Listeria monocytogenes in ruminants. Brain Pathol. 20, 378-390.
  • Referans28Ortatatlı M, Çiftçi MK, Tuzcu M. (2001) Bir koyun sürüsünde görülen purulent ensefalitis. Vet Bil Derg. 17, 97-102.
  • Referans29Özyıldız Z, Dinçel GÇ, Terzi OS, Özsoy ŞY, Kul O. (2018) Immunohistochemical investigation of the damage to and repair of myelin, and astrocyte activity in small ruminants resulting from with natural meningoencephalitic listeriosis. Ankara Üniv Vet Fak Derg. 65, 283-290.
  • Referans30Ralovich B. (1993) Detection and epidemiological typing of Listeria strains – diagnostic methods for Listeria infections (A review). Acta Microbiol Hung. 40, 3-38.
  • Referans31Rocha PR, Lomonaco S, Bottero MT, Dalmasso A, Dondo A, Grattarola C, Zuccon F, Iulini B, Knabel SJ, Capucchio MT, Casalone C. (2013) Ruminant rhombencephalitis-associated Listeria monocytogenes strains constitute a genetically homogeneous group related to human outbreak strains. Appl Environ Microbiol. 79, 3059-3066.
  • Referans32Weinstock D, Horton SB, Rowland PH. (1995) Rapid diagnosis of Listeria monocytogenes by immunohistochemistry in formalin-fixed brain tissue. Vet Pathol. 32, 193-195.
  • Referans33Wesley IV, Larson DJ, Harmon KM, Luchansky JB, Schwartz AR. (2002) A case report of sporadic ovine listerial menigoencephalitis in Iowa with an overview of livestock and human cases. J Vet Diagn Invest. 14, 314-321.

Details

Primary Language English
Subjects Veterinary Surgery
Journal Section Original Article
Authors

Emin KARAKURT
KAFKAS ÜNİVERSİTESİ
0000-0003-2019-3690
Türkiye


Fatih BÜYÜK
KAFKAS ÜNİVERSİTESİ
0000-0003-3278-4834
Türkiye


Serpil DAĞ
KAFKAS ÜNİVERSİTESİ
0000-0001-7667-689X
Türkiye


Enver BEYTUT
KAFKAS ÜNİVERSİTESİ
0000-0003-3360-2940
Türkiye


Özgür ÇELEBİ
KAFKAS ÜNİVERSİTESİ
0000-0002-3478-008X
Türkiye


Özhan KARATAŞ
Cumhuriyet Üniversitesi
0000-0002-2778-8059
Türkiye


Hilmi NUHOĞLU
KAFKAS ÜNİVERSİTESİ
0000-0003-2530-2542
Türkiye


Ali Sefa MENDİL
Erciyes Üniversitesi
0000-0003-2722-3290
Türkiye


Doğan AKÇA
KAFKAS ÜNİVERSİTESİ
0000-0002-3986-8769
Türkiye


Ayfer YILDIZ
KAFKAS ÜNİVERSİTESİ
0000-0002-6569-5435
Türkiye

Early Pub Date January 7, 2021
Publication Date June 30, 2021
Submission Date June 5, 2020
Acceptance Date April 20, 2021
Published in Issue Year 2021 Volume: 32 Issue: 1

Cite

APA Karakurt, E. , Büyük, F. , Dağ, S. , Beytut, E. , Çelebi, Ö. , Karataş, Ö. , Nuhoğlu, H. , Mendil, A. S. , Akça, D. & Yıldız, A. (2021). Investigation of Ruminant Encephalitic and Septicemic Listeriosis by the Immunofluorescence Method . Etlik Veteriner Mikrobiyoloji Dergisi , 32 (1) , 33-39 . DOI: 10.35864/evmd.748570

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